Topical silver diamine fluoride for dental caries arrest in preschool children: A randomized controlled trial and microbiological analysis of caries associated microbes and resistance gene expression

Milgrom, Peter; Horst, Jeremy A.; Ludwig, Sharity; Rothen, Marilynn; Chaffee, Benjamin W.; Lyalina, Svetlana; Pollard, Katherine S.; DeRisi, Joseph L.; Mancl, Lloyd

doi:10.1016/j.jdent.2017.08.015

Cited by 88 publications

(87 citation statements)

References 38 publications

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“…Most of the genera present before the intervention (Olsenella, Streptococcus, Bifidobacterium, Prevotella_7, Lactobacillus, Actinomyces, Leptotrichia, Selenomonas_3, Veillonella) were sensitive to SDF, and the relative amounts decreased substantially. This was consistent with previous studies [15,16,18,20] and supported the broad spectrum bactericidal effect of SDF [25]. Streptococcus,…”

Section: Discussionsupporting

confidence: 93%

“…The antimicrobial effect of SDF has been proven by various previous studies [15][16][17][18][19][20]25], but the effect of SDF on micro-ecology of dental plaque has seldom been explored. Based on the already known antimicrobial effect of SDF, this pioneer preliminary study aimed to further our knowledge of SDF on dental plaque from the micro-ecosystem aspect.…”

Section: Discussionmentioning

confidence: 99%

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Effect of silver diammine fluoride on micro-ecology of plaque from extensive caries of deciduous teeth - in vitro study

Liu¹,

Liu

ZHANG

et al. 2020

Preprint

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Background: The mechanism of action of silver diammine fluoride (SDF) on plaque micro-ecology is seldom studied. This study investigated micro-ecological changes in dental plaque on extensive caries of deciduous teeth after topical SDF treatment. Methods: Deciduous teeth with extensive caries freshly removed from school children were collected in clinic. Unstimulated saliva collection and initial plaque sampling were done before tooth extraction, then each caries was topically treated with 38% SDF in vitro. After intervention, each tooth was stored respectively in artificial saliva at 37℃. Repeated plaque collections were done at 24 hours and 1 week post-intervention. Post-intervention micro-ecological changes including microbial diversity, microbial metabolism function as well as species correlations were analyzed and compared after pyrosequencing of the DNA from the plaque sample using Illumina MiSeq platform. Results: After SDF application, microbial diversity decreased (P>0.05), although not statistically significant. Microbial community composition post-intervention was noticeably different from that of supragingival and pre-intervention plaque as well as saliva. At 1 week post-intervention, the relative content of Pseudomonas, Fusobacterium and Pseudoramibacter was noticeably higher than before, while most of the other bacteria was noticeably reduced, although not statistically significant (P>0.05). The inter-microbial associations became more complex, much more positive associations among survived bacteria were observed than negative ones. COG function classification diagram showed carbohydrate transportation and metabolic functions in the plaque were significantly reduced at 24 hours and 1 week post-intervention. Conclusions: SDF has extensive antimicrobial effect on dental plaque, which may reduce carbohydrate metabolism in dental plaque and help promote new balance of the plaque flora.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Effect of silver diammine fluoride on micro-ecology of plaque from extensive caries of deciduous teeth - in vitro study

Liu¹,

Liu

ZHANG

et al. 2020

Preprint

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“…Another study of preschool children looked at both the impact of 38% SDF on arrest rates and resulting changes in the plaque flora. 232 Similar to related studies, caries arrest was confirmed, but the RNA sequencing of microbes indicated that there was no change in the relative abundance of caries-associated microbes after treatment. While the treatment did not appear to alter the plaque flora, it also did not lead to the emergence of antibiotic or metal-resistant gene expression in the organisms.…”

Section: Silver Diamine Fluoridesupporting

confidence: 77%

Annual review of selected scientific literature: A report of the Committee on Scientific Investigation of the American Academy of Restorative Dentistry

Cagna

Donovan

McKee³

et al. 2019

The Journal of Prosthetic Dentistry

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“…As SDF exerts such a strong antibacterial activity, it has been feared that topical treatments would dramatically reduce diversity and thereby enable growth of unwanted, opportunistic pathogens. In contrast to the expectations, MILGROM and coworkers (44) found no significant loss of species diversity and no changes in the relative abundance of bacteria associated with dental caries after topical application of SDF. The findings need to be validated in larger settings but the authors concluded that SDF treatment seemed to pose a minimal risk of unintended systemic effects on the oral microbiome (44).…”

Section: Antibacterial Strategiesmentioning

confidence: 70%

Prevention of dental caries as a non‐communicable disease

Twetman

2018

European J Oral Sciences

102

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Today, dental caries is regarded as a preventable non-communicable disease (NCD) that affects a majority of the population across their lifespan. As such, it shares a number of behavioural, socio-economic, and lifestyle factors with other NCDs, such as overweight and diabetes, and should be subjected to a similar model of chronic disease management. Caries prevention has traditionally relied on fluoride exposure, diet control, thorough oral hygiene, and antibacterial measures. Prevention of caries as an NCD does certainly not disqualify these methods, but brings them into a new context. This conference paper provides a brief review on how common preventive measures can interfere with the drivers of dysbiosis and promote the growth of health-associated clusters in the oral microbiome. Besides the established routines of regular toothbrushing with fluoride products, there is an opportunity for additional technologies, based on ecological principles, to address and modify the oral biofilm. Methods to reduce dietary sugar intake, slow down plaque metabolism, and support saliva functions should be further developed and investigated in terms of efficacy, compliance, and cost-effectiveness. Furthermore, biofilm engineering through pre- and probiotics early in life to support microbial diversity seem promising in order to obtain a sustained caries-preventive effect.

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Topical silver diamine fluoride for dental caries arrest in preschool children: A randomized controlled trial and microbiological analysis of caries associated microbes and resistance gene expression

Abstract: The treatment is applicable to primary care practice and may reduce the burden of untreated tooth decay in the population.

Cited by 88 publications

References 38 publications

Effect of silver diammine fluoride on micro-ecology of plaque from extensive caries of deciduous teeth - in vitro study

Effect of silver diammine fluoride on micro-ecology of plaque from extensive caries of deciduous teeth - in vitro study

Annual review of selected scientific literature: A report of the Committee on Scientific Investigation of the American Academy of Restorative Dentistry

Prevention of dental caries as a non‐communicable disease

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