TOPOISOMERASE 6B is involved in chromatin remodelling associated with control of carbon partitioning into secondary metabolites and cell walls, and epidermal morphogenesis in Arabidopsis

Mittal, Amandeep; Balasubramanian, Rajagopal; Cao, Jin; Singh, Prabhjeet; Subramanian, Senthil; Hicks, Glenn R.; Nothnagel, Eugene A.; Abidi, Noureddine; Janda, Jaroslav; Rock, Christopher D.

doi:10.1093/jxb/eru198

Cited by 10 publications

(8 citation statements)

References 127 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The expression of a brassinosteroid signaling gene, TOP6B ( BIN3 / HYP6, XLOC_000845 ), was high at S5 stage, which positively regulates cell expansion via controlling DNA replication and endoreduplication levels (Hartung et al ., ; Yin et al ., ). More recently, its role in carbon partitioning into secondary metabolites and cell wall has also been demonstrated (Mittal et al ., ).…”

Section: Resultsmentioning

confidence: 97%

Global transcriptome and coexpression network analyses reveal cultivar‐specific molecular signatures associated with seed development and seed size/weight determination in chickpea

et al. 2017

View full text Add to dashboard Cite

Seed development is an intricate process regulated via a complex transcriptional regulatory network. To understand the molecular mechanisms governing seed development and seed size/weight in chickpea, we performed a comprehensive analysis of transcriptome dynamics during seed development in two cultivars with contrasting seed size/weight (small-seeded, Himchana 1 and large-seeded, JGK 3). Our analysis identified stage-specific expression for a significant proportion (>13%) of the genes in each cultivar. About one half of the total genes exhibited significant differential expression in JGK 3 as compared with Himchana 1. We found that different seed development stages can be delineated by modules of coexpressed genes. A comparative analysis revealed differential developmental stage specificity of some modules between the two cultivars. Furthermore, we constructed transcriptional regulatory networks and identified key components determining seed size/weight. The results suggested that extended period of cell division during embryogenesis and higher level of endoreduplication along with more accumulation of storage compounds during maturation determine large seed size/weight. Further, we identified quantitative trait loci-associated candidate genes harboring single nucleotide polymorphisms in the promoter sequences that differentiate small- and large-seeded chickpea cultivars. The results provide a valuable resource to dissect the role of candidate genes governing seed development and seed size/weight in chickpea.

show abstract

Section: Resultsmentioning

confidence: 97%

Global transcriptome and coexpression network analyses reveal cultivar‐specific molecular signatures associated with seed development and seed size/weight determination in chickpea

et al. 2017

View full text Add to dashboard Cite

show abstract

“… [ 48 ] RHL2 Positive Regulate trichome nucleus replication, regulate trichome morphology. [ 49 ] ICK/KRP Positive Regulate trichome nucleus replication. [ 50 ] HYP6 Positive The mutant has a small number of trichome branches and intranuclear replication (8C).…”

Section: Regulatory Mechanism Of Trichome Development In Plantsmentioning

confidence: 99%

Analysis and review of trichomes in plants

et al. 2021

View full text Add to dashboard Cite

Background Trichomes play a key role in the development of plants and exist in a wide variety of species. Results In this paper, it was reviewed that the structure and morphology characteristics of trichomes, alongside the biological functions and classical regulatory mechanisms of trichome development in plants. The environment factors, hormones, transcription factor, non-coding RNA, etc., play important roles in regulating the initialization, branching, growth, and development of trichomes. In addition, it was further investigated the atypical regulation mechanism in a non-model plant, found that regulating the growth and development of tea (Camellia sinensis) trichome is mainly affected by hormones and the novel regulation factors. Conclusions This review further displayed the complex and differential regulatory networks in trichome initiation and development, provided a reference for basic and applied research on trichomes in plants.

show abstract

“…Besides its role in endoreduplication, the plant Topoisomerase VI has been implicated in transcriptional silencing (Kirik et al, 2007) and gene expression control, notably during the response of plants to stresses and phytohormones (Yin et al, 2002; Jain et al, 2008; Mittal et al, 2014; Jain et al, 2006). For instance, the constitutive expression of the rice Topo VIA or Topo VIB subunits enhanced the expression of stress-responsive genes and conferred abiotic stress tolerance to transgenic Arabidopsis plants (Jain et al 2006).…”

Section: Discussionmentioning

confidence: 99%

TFIIFα interacts with the Topoisomerase VI complex and selectively controls the expression of genes encoding PPR proteins involved in organellar RNA editing in Arabidopsis

Dimnet¹,

Vriet²,

Achard³

et al. 2022

Preprint

View full text Add to dashboard Cite

Communication between organelles and the nucleus is referred to as anterograde (nucleus to organelle) and retrograde (organelle to nucleus) signalling. In plants, the pentatricopeptide repeat (PPR) proteins represent a large family of nuclear-encoded proteins that are required for post-transcriptional control of chloroplast and mitochondria gene expression, and hence play a central role in the nuclear anterograde control of organelle genome expression. How PPR gene expression is controlled and regulated by retrograde signals is, however, still unknown. Here, we report a significant role for the general transcription factor TFIIF α-subunit (TFIIFα) in controlling PPR gene expression in Arabidopsis. First, we found that TFIIFα interacts with the BIN4 subunit of the Topoisomerase VI (Topo VI). Transcriptome analysis of TFIIF and Topo VI mutant lines then revealed that many PLS-type PPR genes involved in RNA editing are reciprocally controlled by TFIIF and Topo VI. The misexpression of CLB19 and DYW1 genes in two allelic tfIIfα mutants was associated with editing impairments in their plastid target RNAs rpoA and ndhD, respectively. Interestingly, we also detected a change in NDH activity in tfIIfα plants. We also show that TFIIFα and Topo VI coordinate the expression of NDH subunits encoded by the nuclear and plastid genomes. These results reveal the crucial role of the nuclear TFIIFα and Topo VI complexes in controlling plastid genome expression at multiple levels of regulation, including the particular regulation of PPR gene expression.

show abstract

TOPOISOMERASE 6B is involved in chromatin remodelling associated with control of carbon partitioning into secondary metabolites and cell walls, and epidermal morphogenesis in Arabidopsis

Abstract: SummaryThe harlequin mutant encodes subunit B of plant-specific topoisomerase VI. Transcriptomics showed synergistic effects on expression of adjacent genes, suggesting a broader role in chromatin remodelling during development and stress responses.

Cited by 10 publications

References 127 publications

Global transcriptome and coexpression network analyses reveal cultivar‐specific molecular signatures associated with seed development and seed size/weight determination in chickpea

Global transcriptome and coexpression network analyses reveal cultivar‐specific molecular signatures associated with seed development and seed size/weight determination in chickpea

Analysis and review of trichomes in plants

TFIIFα interacts with the Topoisomerase VI complex and selectively controls the expression of genes encoding PPR proteins involved in organellar RNA editing in Arabidopsis

Contact Info

Product

Resources

About