Topological properties of the triple gene block protein 2 of Bamboo mosaic virus

Hsu, Hsiu‐Ting; Chou, Yuan‐Lin; Tseng, Yang-Hao; Lin, Yu‐Hsing; Lin, Tzung-Min; Lin, Na-Sheng; Hsu, Yau-Heiu; Chang, Ban‐Yang

doi:10.1016/j.virol.2008.06.019

Cited by 23 publications

(29 citation statements)

References 50 publications

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“…As mentioned earlier, research with GFP–TGB2 and GFP–TGB3 fusion proteins has demonstrated that both TGB2 and TGB3 proteins are integral membrane proteins in the ER or ER-associated vesicles located at actin filaments (Morozov and Solovyev, 2003; Hsu et al, 2008). In relation to the role of TGB2 and TGB3 proteins for potexvirus vRNA trafficking to PD, two models have been proposed (Verchot-Lubicz et al, 2010; Chou et al, 2013).…”

Section: Intracellular Trafficking Of Potexvirus Vrna From the Cytoplmentioning

confidence: 92%

“…A topological study with BaMV showed that potexvirus TGB2 protein integrates into the ER membranes in a U-like structure with the central loop exposed to the ER lumen and with both the N- and C-terminus located on the cytoplasmic side of the ER (Hsu et al, 2008; Figure 1C ; TGB2). Cysteine-to-alanine substitution analysis indicated that two conserved cysteine residues (Cys 109 and Cys 112 ) in the C-terminal region of potexvirus TGB2 protein are critical for both cell-to-cell and systemic movement of BaMV (Hsu et al, 2008; Tseng et al, 2009). In addition, potexvirus TGB2 protein has sequence-independent RNA-binding activity (Cowan et al, 2002; Hsu et al, 2009).…”

Section: Three Tgb Proteins (Tgb1 Tgb2 and Tgb3)mentioning

confidence: 99%

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Understanding the intracellular trafficking and intercellular transport of potexviruses in their host plants

2014

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The movement of potexviruses through the cytoplasm to plasmodesmata (PD) and through PD to adjacent cells depends on the viral and host cellular proteins. Potexviruses encode three movement proteins [referred to as the triple gene block (TGB1–3)]. TGB1 protein moves cell-to-cell through PD and requires TGB2 and TGB3, which are endoplasmic reticulum (ER)-located proteins. TGB3 protein directs the movement of the ER-derived vesicles induced by TGB2 protein from the perinuclear ER to the cortical ER. TGB2 protein physically interacts with TGB3 protein in a membrane-associated form and also interacts with either coat protein (CP) or TGB1 protein at the ER network. Recent studies indicate that potexvirus movement involves the interaction between TGB proteins and CP with host proteins including membrane rafts. A group of host cellular membrane raft proteins, remorins, can serve as a counteracting membrane platform for viral ribonucleoprotein (RNP) docking and can thereby inhibit viral movement. The CP, which is a component of the RNP movement complex, is also critical for viral cell-to-cell movement through the PD. Interactions between TGB1 protein and/or the CP subunit with the 5′-terminus of genomic RNA [viral RNA (vRNA)] form RNP movement complexes and direct the movement of vRNAs through the PD. Recent studies show that tobacco proteins such as NbMPB2C or NbDnaJ-like proteins interact with the stem-loop 1 RNA located at the 5′-terminus of Potato virus X vRNA and regulate intracellular as well as intercellular movement. Although several host proteins that interact with vRNAs or viral proteins and that are crucial for vRNA transport have been screened and characterized, additional host proteins and details of viral movement remain to be characterized. In this review, we describe recent progress in understanding potexvirus movement within and between cells and how such movement is affected by interactions between vRNA/proteins and host proteins.

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Section: Intracellular Trafficking Of Potexvirus Vrna From the Cytoplmentioning

confidence: 92%

Section: Three Tgb Proteins (Tgb1 Tgb2 and Tgb3)mentioning

confidence: 99%

Understanding the intracellular trafficking and intercellular transport of potexviruses in their host plants

2014

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“…This outcome was expected because NbRabG3f participates in BaMV replication, whereas NbRabGAP1 assists in the intercellular movement of BaMV. Similar to PVX, the BaMV movement proteins TGBp2 and TGBp3 are ER-targeting membrane proteins (Hsu et al, 2008). The BaMV infectious complex has been proposed to move from perinuclear ER-derived membrane-bound bodies (MBB) to the plasmodesmata (Wu et al, 2011; Liou et al, 2015).…”

Section: A Host Factor Possibly Involved In Bamv Vesicle Trafficking mentioning

confidence: 99%

Host Factors Involved in the Intracellular Movement of Bamboo mosaic virus

Cheng

2017

Front. Microbiol.

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Viruses move intracellularly to their replication compartments, and the newly synthesized viral complexes are transported to neighboring cells through hijacking of the host endomembrane systems. During these processes, numerous interactions occur among viral proteins, host proteins, and the cytoskeleton system. This review mainly focuses on the plant endomembrane network, which may be utilized by Bamboo mosaic virus (BaMV) to move to its replication compartment, and summarizes the host factors that may be directly involved in delivering BaMV cargoes during intracellular movement. Accumulating evidence indicates that plant endomembrane systems are highly similar but exhibit significant variations from those of other eukaryotic cells. Several Nicotiana benthamiana host proteins have recently been identified to participate in the intracellular movement of BaMV. Chloroplast phosphoglycerate kinase, a host protein transported to chloroplasts, binds to BaMV RNAs and facilitates BaMV replication. NbRABG3f is a small GTPase that plays an essential role in vesicle transportation and is also involved in BaMV replication. These two host proteins may deliver BaMV to the replication compartment. Rab GTPase activation protein 1, which switches Rab GTPase to the inactive conformation, participates in the cell-to-cell movement of BaMV, possibly by trafficking BaMV cargo to neighboring cells after replication. By analyzing the host factors involved in the intracellular movement of BaMV and the current knowledge of plant endomembrane systems, a tentative model for BaMV transport to its replication site within plant cells is proposed.

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“…Results from multiple studies have predicted that TGB2 proteins form a U-shaped membrane domain with the central connecting loop in the ER lumen (e.g. Zamyatnin et al, 2006; Hsu et al, 2008). Consistent with these findings are the results from Zamyatnin et al (2006), who used bimolecular fluorescence complementation (BiFC) to determine that the N- and C-termini of a hordei-like TGB2 protein were located in the cytoplasm.…”

Section: Intracellular Movement Of Plant Viruses With Mps Not Associamentioning

confidence: 99%

Intracellular Transport of Plant Viruses: Finding the Door out of the Cell

2011

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Plant viruses are a class of plant pathogens that specialize in movement from cell to cell. As part of their arsenal for infection of plants, every virus encodes a movement protein (MP), a protein dedicated to enlarging the pore size of plasmodesmata (PD) and actively transporting the viral nucleic acid into the adjacent cell. As our knowledge of intercellular transport has increased, it has become apparent that viruses must also use an active mechanism to target the virus from their site of replication within the cell to the PD. Just as viruses are too large to fit through an unmodified plasmodesma, they are also too large to be freely diffused through the cytoplasm of the cell. Evidence has accumulated now for the involvement of other categories of viral proteins in intracellular movement in addition to the MP, including viral proteins originally associated with replication or gene expression. In this review, we will discuss the strategies that viruses use for intracellular movement from the replication site to the PD, in particular focusing on the role of host membranes for intracellular transport and the coordinated interactions between virus proteins within cells that are necessary for successful virus spread.

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Topological properties of the triple gene block protein 2 of Bamboo mosaic virus

Cited by 23 publications

References 50 publications

Understanding the intracellular trafficking and intercellular transport of potexviruses in their host plants

Understanding the intracellular trafficking and intercellular transport of potexviruses in their host plants

Host Factors Involved in the Intracellular Movement of Bamboo mosaic virus

Intracellular Transport of Plant Viruses: Finding the Door out of the Cell

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