Tospo viral infection instigates necrosis and premature senescence by micro RNA controlled programmed cell death in Vigna unguiculata

Permar, Vipin; Singh, Ajeet; Pandey, Vanita; Alatar, Abdulrahman A.; Faisal, Mohammad; Jain, Rakesh; Praveen, Shelly

doi:10.1016/j.pmpp.2014.09.004

Cited by 22 publications

(11 citation statements)

References 41 publications

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“…The downregulation of miR482 and upregulation of NB-LRR transcripts at the infection sites were observed in tomato plants infected with turnip crinkle virus (TCV), cucumber mosaic virus (CMV) and tobacco rattle virus (TRV) (Shivaprasad et al 2012) and in cowpea (Vigna unguiculata (L.) Walp.) plants infected with groundnut bud necrosis virus (GBNV) (Permar et al 2014). Previous studies have indicated that miR172 might be linked to leaf curl disease in tomato caused by tomato leaf curl New Delhi virus infection (Naqvi et al 2010).…”

Section: Discussionmentioning

confidence: 99%

“…Plant miRNAs are small endogenous non-coding RNAs that post-transcriptionally regulate gene expression by targeting specific mRNA for cleavage or translational inhibition (Bartel 2004;Voinnet 2009); they play essential roles in plant development and responses to biotic and abiotic stresses (Ruiz-Ferrer and Voinnet 2009;Khraiwesh et al 2012;Ramesh et al 2014;Yin et al 2014). Certain miRNAs are involved in antiviral immunity by regulating resistance (R) genes that encode proteins containing nucleotide binding (NB) and leucine-rich repeat (LRR) domains (Li et al 2012;Shivaprasad et al 2012;Permar et al 2014;Chen et al 2016a, b). Previously, we showed that a set of potato miRNAs and targets was altered in cv.…”

Section: Introductionmentioning

confidence: 99%

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Accumulation of miRNA and mRNA Targets in Potato Leaves Displaying Temperature-Dependent Responses to Potato Virus Y

2019

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We demonstrate temporal-, spatial-and temperature-dependent expression of five host microRNAs and target mRNAs in potato leaves after potato virus Y (PVY) inoculation. A novel gene, Ny-DG, conferring resistance to PVY was mapped on chromosome IX in the diploid potato clone DG 81-68. At 20°C, the PVY NTN strain was localized in the inoculated leaves without symptoms. At 28°C, necrotic lesions were observed in both the inoculated leaves and the non-inoculated upper leaves and the virus spread systemically, which indicates overcoming the resistance. At 0.5 h post-inoculation and 3-day postinoculation (dpi), PVY led to significant changes in the levels of the tested miRNAs and their targets only in a small number of cases. At 6 dpi, in the inoculated leaves at 28°C, the increased expression of stu-miR162, stu-miR168a and stu-miR482 (PotatoMir1005353123_x15854) promoted the downregulation of their targets DCL1, AGO1-2 and Cc-nbs-lrr, respectively. The expression of stu-miR482 (PotatoMir1005658171_x16170) and stu-miR172e remained unchanged, whereas their targets Gpa2 and TOE3 were downregulated. However, in the inoculated leaves at 20°C, all five tested miRNAs and their targets showed parallel downregulation. In the noninoculated upper leaves, changes in gene expression levels, namely downregulation, were only detected for the tested five miRNAs at 28°C. We conclude that the expression patterns of the tested miRNAs and targets were altered differently not only at different time points post-inoculation but also in the inoculated and upper leaves, and their accumulation levels were related to the type of reaction, which in turn was dependent on temperature.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Accumulation of miRNA and mRNA Targets in Potato Leaves Displaying Temperature-Dependent Responses to Potato Virus Y

2019

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“…In tobacco ( Nicotiana tabacum ), coexpression of the NB‐LRR type N gene with nta‐miR6019 and nta‐miR6020 was shown to result in the attenuation of N ‐mediated resistance to Tobacco mosaic virus (TMV), indicating that these miRNAs have functional roles in NB‐LRR regulation (Li et al ., ). In cowpea ( Vigna unguiculata ), Groundnut bud necrosis virus (GBNV) infection caused down‐regulation of miR482 and up‐regulation of NB‐LRR transcripts at the infection site, indicating the activation of host disease defence (Permar et al ., ). It has been proposed that virus‐inducible expression of NB‐LRR proteins is mediated by RNA‐silencing suppressors (RSSs) encoded by plant viruses.…”

Section: Introductionmentioning

confidence: 97%

Potato cultivar Etola exhibits hypersensitive resistance to PVY^NTN and partial resistance to PVY^Z‐NTN and PVY^N‐Wi strains and strain‐specific alterations of certain host miRNAs might correlate with symptom severity

et al. 2016

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This study demonstrates the new sources of resistance available in potato cv. Etola, which represent multiple strain-specific resistance genes against three strains of Potato virus Y (PVY). Etola is the first documented cultivar of this sort in Europe that exhibits hypersensitive resistance (HR) to PVY NTN isolate PVY-3202, and different levels of partial resistance to PVY N-Wi isolate PVY-3411 and PVY Z -NTN isolate PVY-3303. The isolate PVY-3411 induced severe symptoms and faster systemic viral coat protein and RNA accumulation in the noninoculated upper leaves, whereas PVY-3303 caused mild symptoms and delayed viral spreading. This study provides the first example of strain-specific alteration of a set of host microRNAs (miRNAs) and their targets in the potato-PVY interaction. The tested miRNAs and targets are altered only in plants of cv. Etola infected with PVY-3411 but not those infected with PVY-3303 nor PVY-3202. The up-regulation of stu-miR162, stu-miR168a and miR172e, together with their target transcripts, DCL1, AGO1-2 and TOE3, respectively, in PVY-3411-infected plants correlates with high abundance of HC-Pro RNA encoding an RNA-silencing suppressor and might be linked with the severe symptoms in leaves. Moreover, PVY-3411 causes parallel increases in two members of stu-miR482 and their mRNA targets Gpa2 and CC-NBS-LRR that are involved in the defence response.

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“…The HR in response to virus infection is a typical example of PCD (Greenberg and Yao, 2004 ). Increased cell membrane damage and cell death have been well established in the plants infected with various viruses such as Tobacco mosaic virus (TMV)-infected Nicotiana tabacum (del Pozo and Lam, 2003 ), Groundnut bud necrosis virus (GBNV)-infected Vigna unguiculata (Permar et al, 2014 ) and Soybean mosaic virus (SMV)-infected Glycine max (Zhang et al, 2014 ). The HR is typically induced upon virus infection as in interaction between host encoded resistance (R) proteins and pathogen encoded avirulence proteins and functions to limit virus replication and thereby its eventual movement inside the host (Padder, 2014 ).…”

Section: Discussionmentioning

confidence: 99%

Responses of In vitro-Grown Plantlets (Vitis vinifera) to Grapevine leafroll-Associated Virus-3 and PEG-Induced Drought Stress

Cui

Hao

et al. 2016

Front. Physiol.

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Stresses caused by viral diseases and drought have long threatened sustainable production of grapevine. These two stresses frequently occur simultaneously in many of grapevine growing regions of the world. We studied responses of in vitro-grown plantlets (Vitis vinifera) to Grapevine leafroll associated virus-3 (GLRaV-3) and PEG-induced drought stress. Results showed that stress induced by either virus infection or drought had negative effects on vegetative growth, caused significant decreases and increases in total soluble protein and free proline, respectively, induced obvious cell membrane damage and cell death, and markedly increased accumulations of O2·- and H2O2. Co-stress by virus and drought had much severer effects than single stress on the said parameters. Virus infection alone did not cause significant alternations in activities of POD, ROS, and SOD, and contents of MDA, which, however, markedly increased in the plantlets when grown under single drought stress and co-stress by the virus and drought. Levels of ABA increased, while those of IAA decreased in the plantlets stressed by virus infection or drought. Simultaneous stresses by the virus and drought had co-effects on the levels of ABA and IAA. Up-regulation of expressions of ABA biosynthesis genes and down-regulation of expressions of IAA biosynthesis genes were responsible for the alternations of ABA and IAA levels induced by either the virus infection or drought stress and co-stress by them. Experimental strategies established in the present study using in vitro system facilitate investigations on ‘pure’ biotic and abiotic stress on plants. The results obtained here provide new insights into adverse effects of stress induced by virus and drought, in single and particularly their combination, on plants, and allow us to re-orientate agricultural managements toward sustainable development of the agriculture.

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Tospo viral infection instigates necrosis and premature senescence by micro RNA controlled programmed cell death in Vigna unguiculata

Cited by 22 publications

References 41 publications

Accumulation of miRNA and mRNA Targets in Potato Leaves Displaying Temperature-Dependent Responses to Potato Virus Y

Accumulation of miRNA and mRNA Targets in Potato Leaves Displaying Temperature-Dependent Responses to Potato Virus Y

Potato cultivar Etola exhibits hypersensitive resistance to PVY^NTN and partial resistance to PVY^Z‐NTN and PVY^N‐Wi strains and strain‐specific alterations of certain host miRNAs might correlate with symptom severity

Responses of In vitro-Grown Plantlets (Vitis vinifera) to Grapevine leafroll-Associated Virus-3 and PEG-Induced Drought Stress

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Tospo viral infection instigates necrosis and premature senescence by micro RNA controlled programmed cell death in Vigna unguiculata

Cited by 22 publications

References 41 publications

Accumulation of miRNA and mRNA Targets in Potato Leaves Displaying Temperature-Dependent Responses to Potato Virus Y

Accumulation of miRNA and mRNA Targets in Potato Leaves Displaying Temperature-Dependent Responses to Potato Virus Y

Potato cultivar Etola exhibits hypersensitive resistance to PVYNTN and partial resistance to PVYZ‐NTN and PVYN‐Wi strains and strain‐specific alterations of certain host miRNAs might correlate with symptom severity

Responses of In vitro-Grown Plantlets (Vitis vinifera) to Grapevine leafroll-Associated Virus-3 and PEG-Induced Drought Stress

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Potato cultivar Etola exhibits hypersensitive resistance to PVY^NTN and partial resistance to PVY^Z‐NTN and PVY^N‐Wi strains and strain‐specific alterations of certain host miRNAs might correlate with symptom severity