Total extract of Beta Vulgaris var. Cicla seeds versus its purified phenolic components: antioxidant activities and antiproliferative effects against colon cancer cells

Abstract

Xylosylvitexin is the main and more efficient chemopreventive compound in BV seeds, but the natural cocktail of molecules, represented by P4 fraction, showed a better compromise between the antiproliferative activity on RKO cells and the enhancement of HF proliferation.

FREE RADICALS AND ANTIOXIDANTS: ANTIOXIDATIVE AND OTHER PROPERTIES OF SWISS CHARD (Beta vulgaris L. subsp. cicla)

“…All of them were tested for cytostatic and cytotoxic activity on RKO and HF cells. The ratio of antioxidant activity determined by ORAC method and phenolics showed an increasing of the antioxidant activity along the purification process (Gennari et al, 2011). Xylosylvitexin, the main and more efficient chemopreventive compound in Swiss chard seeds, represented by 39.44% in the phenolic fraction, exhibited the strongest antiproliferative activity on RKO cells with an IC 50 of 89  9 mg/mL, together with an enhancement of the apoptosis, an increase of cells in the G1 phase and a reduction of cells in the S phase.…”

“…Finally, xylosylvitexin is the main and more efficient chemopreventive compound in B. vulgaris var. cicla seeds, but the natural mixture of molecules, represented by phenolic fraction, showed a better compromise between the antiproliferative activity on RKO cells and the enhancement of HF proliferation (Gennari et al, 2011).…”

“…However, the leaves as a source of these compounds are easily degradable during storage in fresh conditions and a drying process requires time and a large amount of energy. Due to these facts, the extraction of apigeninglycosides is better to be conveniently performed from the seeds, which represent a stable and year-long available biomass (Gennari et al, 2011). Gennari et al found a strategy of purification of bioactive flavonoids from the seeds and tested their ability to inhibit proliferation on human colon cancer (RKO) cells and normal human fibroblasts (HF).…”

“…Nutritional quality and chemical composition of different varieties and cultivars of Beta vulgaris subsp. cicla were investigated including antioxidant, anti-diabetic, anticancer and some other biological activities (Ninfali et al 2007;Ninfali and Angelino, 2013;Pyo et al, 2004;Sacan and Yanardaǧ, 2010;Gil et al, 1998;Bozokalfa et al, 2011;Tomás-Callejas et al, 2011;Pokluda and Kuben, 2002;Gennari et al, 2011;Santos et al, 2014;Yanardaǧ and Çolak, 1998;Bolkent et al, 2000;Ninfali and Bacchiocca, 2003). Phenolic content and antioxidant activities have been most frequently studied.…”

“…Regarding antioxidative properties, Beta vulgaris subsp. cicla was also estimated few times (Pyo et al, 2004;Gennari et al, 2011;Sacan and Yanardag, 2010). These data together with evidences about chemical composition of Swiss chard are reviewed in this paper, besides some general characteristics of free radicals and nutrient antioxidants.…”