Total Flavonoids of Rhizoma Drynariae Ameliorate Bone Growth in Experimentally Induced Tibial Dyschondroplasia in Chickens via Regulation of OPG/RANKL Axis

Xu, Tingting; Zheng, Jingjing; Jin, WeiXing; Li, Lü; Lin, Luxi; Shaukat, Aftab; Zhang, Chaodong; Cao, Qi; Ashraf, Muhammad; Huang, Shucheng

doi:10.3389/fphar.2022.881057

Cited by 8 publications

(6 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our results indicated that tibial bone strength, Ca and P metabolism disorder, and bone formation marker ALP decreased in TD broilers. The results of this study are consistent with the findings of Xu et al [ 37 ] (2018) and Liu et al [ 42 ] (2021). Many studies have proved that TCM has the function of tonifying the kidneys and strengthening the bones.…”

Section: Discussionsupporting

confidence: 93%

“…TD is a susceptible disease of the tibial tarsus affecting the proximal growth plate of the tibia in fast-growing poultry, and its prevention and treatment have not been well defined. Numerous studies have confirmed that traditional Chinese herbal medicine plays an important role in improving bone development, such as total flavonoids of Rhizome drynariae [ 37 ], Puerarin [ 38 , 39 ], and Astragalus membranaceus [ 18 ]. We investigated the therapeutic effects of MOP on TD broilers, and the results showed that MOP could improve tibia-related indicators through BMP/Smads signaling pathway, improve the disturbance of Ca and P metabolism caused by TD, and increase the antioxidant capacity of the chickens.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Morinda officinalis Polysaccharides Ameliorates Bone Growth by Attenuating Oxidative Stress and Regulating the Gut Microbiota in Thiram-Induced Tibial Dyschondroplasia Chickens

Zhang

Lin

et al. 2022

Metabolites

View full text Add to dashboard Cite

Tibial dyschondroplasia (TD) occurs in chickens and other fast-growing birds, affecting their cartilage growth and leading to reduced meat quality in broilers. Morinda officinalis polysaccharide (MOP) is one of the chief active components of Morinda officinalis, which promotes bone formation, inhibiting bone loss and having anti-oxidant and anti-inflammatory properties. A total of 120 AA chickens were randomly divided into the CON group (basal diet), TD group (100 mg/kg thiram + basal diet), and MOP group (100 mg/kg thiram + basal diet + water with 500 mg/kg MOP). The experiment lasted 21 days. The results showed that MOP could alleviates broiler lameness caused by TD, restore the morphological structure of tibial growth plate (TGP), increase tibial weight (p < 0.05), balance the disorder of calcium and phosphorus metabolism, and promote bone formation by increasing the expression of BMP-2, Smad4, and Runx2 genes In addition, MOP supplementation stimulated the secretion of plasma antioxidant enzymes (T-SOD and GSH-Px) by regulating the expression of SOD and GPX-1 genes, thereby enhancing the antioxidant capacity of TD broilers. Interestingly, we observed MOP can also improve gut microbiota by increasing the beneficial bacteria count and decreasing the harmful bacteria count. These findings indicated that MOP can regulate bone formation through the BMP/Smads signaling pathway, attenuating oxidative stress and regulating the gut microbiota of TD broilers, so as to achieve the effect of treating TD. This suggests that MOP might be a potential novel drug in the treatment of TD in chickens.

show abstract

Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Morinda officinalis Polysaccharides Ameliorates Bone Growth by Attenuating Oxidative Stress and Regulating the Gut Microbiota in Thiram-Induced Tibial Dyschondroplasia Chickens

Zhang

Lin

et al. 2022

Metabolites

View full text Add to dashboard Cite

show abstract

“…AFB1 is a class of highly toxic mycotoxins, and the waste of feed ingredients and decreased animal performance caused by AFB1 contamination have brought heavy economic losses to the livestock industry [ 36 ]. As a flavonoid herbal medicine extract, TFRD has various bioactive effects, including immunomodulatory, anti-inflammatory, antioxidant, and anti-apoptotic effects [ 1 , 5 , 29 , 31 ]. In this present experiment, the main active ingredient of TFRD is Rutin .…”

Section: Discussionmentioning

confidence: 99%

“…TFRD (#K20798) had been acquired from Xi’an Kailai Biological Engineering Co., Ltd. (Xi’an, China). The compositional analysis of TFRD is shown in Figure S1 and Table S1 , and its main component was detected by liquid chromatography-mass spectrometry (LC-MS) analysis as Rutin (97.82%) [ 29 ]. Hematoxylin and eosin (HE) and Periodic Acid-Schiff (PAS) were purchased from Servicebio ® (Wuhan, China).…”

Section: Methodsmentioning

confidence: 99%

Total Flavonoids of Rhizoma Drynariae Mitigates Aflatoxin B1-Induced Liver Toxicity in Chickens via Microbiota-Gut-Liver Axis Interaction Mechanisms

et al. 2023

Self Cite

View full text Add to dashboard Cite

Aflatoxin B1 (AFB1) is a common mycotoxin that widely occurs in feed and has severe hepatotoxic effects both in humans and animals. Total flavonoids of Rhizoma Drynaria (TFRD), a traditional Chinese medicinal herb, have multiple biological activities and potential hepatoprotective activity. This study investigated the protective effects and potential mechanisms of TFRD against AFB1-induced liver injury. The results revealed that supplementation with TFRD markedly lessened broiler intestinal permeability by increasing the expression of intestinal tight junction proteins, as well as correcting the changes in gut microbiota and liver damage induced by AFB1. Metabolomics analysis revealed that the alterations in plasma metabolites, especially taurolithocholic acid, were significantly improved by TFRD treatment in AFB1-exposed chickens. In addition, these metabolites were closely associated with [Ruminococcus], ACC, and GPX1, indicating that AFB1 may cause liver injury by inducing bile acid metabolism involving the microbiota–gut–liver axis. We further found that TFRD treatment markedly suppressed oxidative stress and hepatic lipid deposition, increased plasma glutathione (GSH) concentrations, and reversed hepatic ferroptosis gene expression. Collectively, these findings indicate that ferroptosis might contribute to the hepatotoxicity of AFB1-exposed chickens through the microbiota–gut–liver axis interaction mechanisms; furthermore, TFRD was confirmed as an herbal extract that could potentially antagonize mycotoxins detrimental effects.

show abstract

“…TFRD extract is derived from the roots of Drynaria roosii Nakaike 74 . It has been indicated to be useful in the treatment and prevention of bone disorders by increasing the proliferation and differentiation of osteoblasts and promoting bone strength 75 , 76 . In the present study, after 14 days of TFRD treatment, TD symptoms in the TFRD group were significantly reduced, accompanied by the restoration of GM homoeostasis, and the GLU levels and the pancreas almost returned to normal.…”

Section: Discussionmentioning

confidence: 99%

Gut microbiome dysregulation drives bone damage in broiler tibial dyschondroplasia by disrupting glucose homeostasis

Chen

Zhang

et al. 2023

npj Biofilms Microbiomes

Self Cite

View full text Add to dashboard Cite

Tibial dyschondroplasia (TD) with multiple incentives is a metabolic skeletal disease that occurs in fast-growing broilers. Perturbations in the gut microbiota (GM) have been shown to affect bone homoeostasis, but the mechanisms by which GM modulates bone metabolism in TD broilers remain unknown. Here, using a broiler model of TD, we noted elevated blood glucose (GLU) levels in TD broilers, accompanied by alterations in the pancreatic structure and secretory function and damaged intestinal barrier function. Importantly, faecal microbiota transplantation (FMT) of gut microbes from normal donors rehabilitated the GM and decreased the elevated GLU levels in TD broilers. A high GLU level is a predisposing factor to bone disease, suggesting that GM dysbiosis-mediated hyperglycaemia might be involved in bone regulation. 16S rRNA gene sequencing and short-chain fatty acid analysis revealed that the significantly increased level of the metabolite butyric acid derived from the genera Blautia and Coprococcus regulated GLU levels in TD broilers by binding to GPR109A in the pancreas. Tibial studies showed reduced expression of vascular regulatory factors (including PI3K, AKT and VEFGA) based on transcriptomics analysis and reduced vascular distribution, contributing to nonvascularization of cartilage in the proximal tibial growth plate of TD broilers with elevated GLU levels. Additionally, treatment with the total flavonoids from Rhizoma drynariae further validated the improvement in bone homoeostasis in TD broilers by regulating GLU levels through the regulation of GM to subsequently improve intestinal and pancreatic function. These findings clarify the critical role of GM-mediated changes in GLU levels via the gut–pancreas axis in bone homoeostasis in TD chickens.

show abstract

Total Flavonoids of Rhizoma Drynariae Ameliorate Bone Growth in Experimentally Induced Tibial Dyschondroplasia in Chickens via Regulation of OPG/RANKL Axis

Cited by 8 publications

References 41 publications

Morinda officinalis Polysaccharides Ameliorates Bone Growth by Attenuating Oxidative Stress and Regulating the Gut Microbiota in Thiram-Induced Tibial Dyschondroplasia Chickens

Morinda officinalis Polysaccharides Ameliorates Bone Growth by Attenuating Oxidative Stress and Regulating the Gut Microbiota in Thiram-Induced Tibial Dyschondroplasia Chickens

Total Flavonoids of Rhizoma Drynariae Mitigates Aflatoxin B1-Induced Liver Toxicity in Chickens via Microbiota-Gut-Liver Axis Interaction Mechanisms

Gut microbiome dysregulation drives bone damage in broiler tibial dyschondroplasia by disrupting glucose homeostasis

Contact Info

Product

Resources

About