Total Hydroperoxide and Biological Antioxidant Potentials in a Neonatal Sepsis Model

Abstract

Oxidant/antioxidant imbalance plays an important role in septic shock. The present study examined changes in circulating oxidative components in a neonatal sepsis model. Subjects were 14 newborn mixed-strain piglets randomly divided into two groups: a cecal ligation and perforation (CLP) model (n ϭ 7) and sham (n ϭ 7). Blood samples for total hydroperoxide (TH), biological antioxidant potential (BAP), tumor necrosis factor (TNF) ␣, interleukin (IL)-6, and IL-10 were collected pre-CLP and at 1, 3, and 6 h post-CLP. TH and BAP levels at 1 h post-CLP were significantly higher in the CLP group than in the sham group. In the CLP group, TH decreased gradually and reached baseline levels by 6 h post-CLP, while BAP remained elevated. Linear correlations were identified between serum TH and BAP at 1 h post-CLP, serum TH and TNF-␣ at 1 h post-CLP, and BAP and IL-6 at 6 h post-CLP. Changes in and correlations between circulating oxidative and inflammatory state components in a neonatal sepsis model were clarified. This is the first study to reveal that the presence of oxidant/antioxidant imbalance in sepsis and septic shock changes during the disease course.

Endothelin receptor antagonist attenuates oxidative stress in a neonatal sepsis piglet model

“…In our previous study, a neonatal sepsis model similar to that used here exhibited gradual increases in TH and IL-6 during the experimental course (17). This was associated with elevated levels of cytokines such as tumor necrosis factor-α and highly mobile group-1 box protein produced by monocytes and macrophages (18,19).…”

“…Due to the early observed TH surge at 1 h post-CLP, experienced by us while using this model, we selected 30 min post-CLP to be the starting time for treatment (17). Because we used piglets from a local farm instead of commercially available piglets, our animals had been exposed to bacterial antigens in their native environments (40).…”

Hospitalized Infants Who Hurt: A Sweet Solution With Oral Sucrose

“…2012;32 [1]: [61][62][63][64][65][66][67][68][69] radicals. [9][10][11][12][13][14][15][16][17][18][19][20] The destructive interplay of pain, hypoxia, and toxic effects caused by free radicals leads to poor outcomes. Table 1 lists conditions of newborns that are associated with these effects.…”

“…Table 1 lists conditions of newborns that are associated with these effects. [9][10][11][12][13][14][15][16][17][18][19][20] Nurses must consider the long-term deleterious effects of repeated unmanaged pain for patients in the NICU, including preterm babies. 9 Table 2 lists several routine invasive and painful procedures infants must undergo during hospitalization.…”

Immunoadsorption therapy reduces oxidative stress in patients with dilated cardiomyopathy

“…The d-ROM level represents the total amount of peroxidized metabolites [16], and BAP reflects serum antioxidant capacity [17]. Values for d-ROM and BAP were ascertained by the Free Radical Elective Evaluator (Diacron International, Grosseto, Italy) using commercial assay kits (Diacron srl, Grosseto, Italy).…”