Total internal reflection ellipsometry for kinetics-based assessment of bovine serum albumin immobilization on ZnO nanowires

Abstract

The properties of the ZnO-NWs were investigated by utilizing spectroscopic ellipsometry under TIRE configuration during real-time measurement of covalent BSA immobilization.

Investigation of SARS-CoV-2 nucleocapsid protein interaction with a specific antibody by combined spectroscopic ellipsometry and quartz crystal microbalance with dissipation

“…For the evaluation of SCoV2-N covalent immobilization on 11-MUA SAM and SCoV2-N/anti-SCoV2-N complex formation on the gold-coated sensor disk, measurements of ellipsometric parameters ( Δ and Ψ ) were performed simultaneously with QCM-D signals registration (Δ F and Δ D ). Here we present only the kinetics of the ellipsometric parameter Δ due to its higher sensitivity [16] , [22] , [39] . The simulation of ellipsometric parameters Ψ and Δ vs λ has been conducted to determine the difference between the optical properties in case of full coverage by SCoV2-N and anti-SCoV2-N versus partial coverage by the same proteins.…”

“…This model consisted of a thick QCM-D gold substrate, thickness d (gold) = 200 nm, covered by 11-MUA SAM, d (SAM) = 0.8 nm and d (SCoV2-N) = 5.9 nm SCoV2-N layers. The refractive index dispersion of the QCM-D gold-coated sensor substrate was modelled using B-Spline function [24] , 11-MUA SAM was modelled by Cauchy dispersion ( n =1.450) and the SCoV2-N monolayer was described using the Bruggeman effective media approach (EMA) [22] , [39] , [40] , [41] .…”

Spectroscopic Ellipsometry and Quartz Crystal Microbalance with Dissipation for the Assessment of Polymer Layers and for the Application in Biosensing

“…Ellipsometry in total internal reflection mode (TIRE) is a powerful tool for applications in biosensing [32,33,69,70]. In many cases during the application of TIRE, the high sensitivity of SE is combined with that of the surface plasmon resonance (SPR).…”

Advances and insights in the diagnosis of viral infections

“…However, in most photoluminescence-based ways, additional photoluminescence probes should be applied, which sometimes is a significant drawback because they need additional expensive chemicals such as antibodies conjugated with photoluminescence probe [ 314 – 316 ]. This drawback can be avoided when Surface Plasmon Resonance (SPR) based sensors [ 309 – 311 , 317 ] and spectroscopic ellipsometry [ 318 – 325 ] based methods are applied. In addition, these sensors are less sophisticated in comparison with more sensitive optical methods.…”