Total Panax notoginseng saponin inhibits balloon injury-induced neointimal hyperplasia in rat carotid artery models by suppressing pERK/p38 MAPK pathways

Abstract

Total Panax notoginseng saponin (TPNS) is the main bioactivity compound derived from the roots and rhizomes of Panax notoginseng (Burk.) F.H. Chen. The aim of this study was to investigate the effectiveness of TPNS in treating vascular neointimal hyperplasia in rats and its mechanisms. Male Sprague-Dawley rats were randomly divided into five groups, sham (control), injury, and low, medium, and high dose TPNS (5, 10, and 20 mg/kg). An in vivo 2F Fogarty balloon-induced carotid artery injury model was established in rats. TPNS significantly and dose-dependently reduced balloon injury-induced neointimal area (NIA) (P<0.001, for all doses) and NIA/media area (MA) (P<0.030, for all doses) in the carotid artery of rats, and PCNA expression (P<0.001, all). The mRNA expression of smooth muscle (SM) α-actin was significantly increased in all TPNS groups (P<0.005, for all doses) and the protein expression was significantly increased in the medium (P=0.006) and high dose TPNS (P=0.002) groups compared to the injury group. All the TPNS doses significantly decreased the mRNA expression of c-fos (P<0.001). The medium and high dose TPNS groups significantly suppressed the upregulation of pERK1/2 protein in the NIA (P<0.025) and MA (P<0.004). TPNS dose-dependently inhibited balloon injury-induced activation of pERK/p38MAPK signaling in the carotid artery. TPNS could be a promising agent in inhibiting cell proliferation following vascular injuries.

Identification of the Crucial Gene in Overflow Arteriovenous Fistula by Bioinformatics Analysis

“…Recent studies reported that the MAPK signaling pathway is associated with intimal hyperplasia by the ERK1/2 signaling pathway (Qu et al, 2020 ). The inhibition of the MAPK pathway decreases the thickening of the intima and limits vascular remodeling (Ge et al, 2013 ; Yang et al, 2020 ). NOD-like receptor signaling pathway could trigger a family of pattern recognition receptors that mediate the initial innate immune response to cellular injury and stress.…”

“…TGF-beta has been shown to induce the expression of DUSP1 , which leads to the inactivation of JNK or p38 MAPK signaling pathway (Xiao et al, 2002 ; Jono et al, 2003 ; Mikami et al, 2006 ). The inhibition of MAPK signaling pathway could suppress cell proliferation and intimal hyperplasia (Ge et al, 2013 ; Qu et al, 2020 ; Yang et al, 2020 ). In our study, the biological processes and pathways in AV fistula with the low expression of DUSP1 were partly concentrated on cell proliferation, indicating the probable promotion of cell proliferation by the p38 MAPK pathway.…”

Efficacy and safety of Qi-Jing Hui-Xin Decoction in the treatment of coronary microvascular angina: study protocol for a randomized, controlled, multi-center clinical trial

“…Meanwhile, the other main herb of this formula, Polygonatum, which contains Polygonatum sibiricum polysaccharides (PSP), has anti-inflammatory and anti-oxidative stress effects [1,23], which may prevent the development of CMD by inhibiting the occurrence of an inflammatory response. In addition, some studies have shown that PSP can also protect endothelial cells [24]. Panax notoginseng Saponins, the main component of Panax ginseng, inhibits intimal hyperplasia in rat arteries by suppressing the pERK/ p38mapk pathway [25].…”