Total Phenolic, Total Flavonoid Content and in vitro Sun Protection Factor test of Arabica Coffee Leaves Extract (Coffea arabica L)

Abstract

Background: Arabica coffee leaf (Coffea arabica L.) is a well-known plant by the Indonesian people because it has many benefits apart from being a drink. Coffee leaves are known to have good antioxidant activity. The use of antioxidants in sunscreen preparations can prevent various diseases caused by UV radiation from UV rays’ sunlight.
 Aim: This study aims to determine the phenol content, the flavonoid content and the value of Sun Protection Factor (SPF) from differences between solvent extracts of arabica coffee leaves (Coffea arabica L.).
 Method: The extraction method used was maceration with solvents: 70% ethanol, 96% ethanol and methanol. Determination of phenol content was carried out using the Folin ciocalteu method. Determination of flavonoid content was done by the AlCl3 method. SPF value is based on the Mansur equation from absorbance scanning using UV Spectrophotometry.
 Result: The results obtained were the highest phenol and flavonoid content is in the methanolic extract of arabica coffee namely 62.371 ± 0.47 mgGAE/g and 8.6707 ± 0.04 mgQE/g. In the SPF test, the highest value was obtained from 70% ethanol extract of 5.0593 ± 1.28 at 100 ppm extract concentration with intermediate protection category.
 Conclusion: Arabica coffee leaves have the potential as an active ingredient in herbal sunscreens

Effect of Light, Medium, and Dark Roasting on Antioxidant Activity of Gununghalu Arabica Coffee (Coffea arabica L.)

“…The results of the phytochemical content test in Table 1 show that the Gununghalu Arabica coffee beans produced from the light roasting, medium roasting, and dark roasting techniques contain flavonoid, alkaloid, terpenoid, saponin, and tannin groups compound, these results are consistent with several previous studies (Fatmawati et al, 2021;Meynderth and Noviastuti 2022;Ali et al, 2022;Harahap et al, 2021).…”

hytochemical Analysis and Determination of MIC and MFC of Cacao Leaves Extract (Theobroma cacao L.) against Malassezia furfur

“…Sementara itu, alkaloid mengandung atom-atom nitrogen yang memiliki pasangan elektron bebas, yang dapat membentuk ikatan kovalen koordinasi dengan ion logam. Ketika direaksikan dengan pereaksi Mayer, atom nitrogen yang dimiliki alkaloid bereaksi dengan ion logam kalium (K + ) dalam kalium tetraiodomerkurat(II) membentuk kompleks kalium-alkaloid (Fatmawati et al, 2022;Hanifa et al, 2021;Sabdoningrum et al, 2021).…”

“…Hasil positif polifenol dengan penambahan FeCl 3 ditandai dengan perubahan warna larutan menjadi gelap, yang disebabkan akibat terjadinya reduksi ion Fe 3+ menjadi Fe 2+ ketika polifenol direaksikan dengan FeCl 3 . Warna biru tua menandakan larutan memiliki senyawa metabolit tanin terhidrolisis, Phytochemical Analysis and Determination of MIC and MFC of Cacao Leaves Extract (Theobroma cacao L.) against Malassezia furfur sedangkan warna hijau kehitaman menandakan larutan memiliki senyawa metabolit tanin terkondensasi (Fatmawati et al, 2022). Setelah direaksikan, larutan uji memiliki warna hijau kehitaman, menandakan ekstrak etanol daun cokelat positif mengandung tanin terkondensasi.…”

“…Terbentuknya busa disebabkan saponin yang mengandung senyawa polar dan non-polar yang membentuk misel, di mana misel-misel tersebut menyebabkan senyawa polar muncul di permukaan, sedangkan senyawa non-polar menghadap ke dalam. Penambahan HCl 2N dimaksudkan untuk meningkatkan kepolaran, sehingga gugus hidrofilik memiliki ikatan yang lebih kuat dan busa yang terbentuk menjadi lebih stabil (Dewi et al, 2019;Fatmawati et al, 2022;Sabdoningrum et al, 2021). Hasil skrining fitokimia pada penelitian ini dapat dilihat pada Tabel 1.…”