Ekstrak etanol daun kersen (Muntingia calabura L.) telah lama digunakan sebagai tanaman obat. Penelitian ini bertujuan untuk mengetahui fraksi dari ekstrak etanol daun kersen yang memiliki efek antiinflamasi melalui parameter penurunan volume eksudat, penurunan jumlah leukosit, monosit, neutrofil, dan limfosit eksudat pada tikus putih jantan udem yang diinduksi karagenin. Hewan percobaan dibagi menjadi lima kelompok yaitu kelompok I (kontrol negatif diberi Na CMC 0,5%), kelompok II (kontrol positif diberi natrium diklofenak 10,278 mg/kgBB), kelompok III, IV, dan V diberi fraksi n-heksana, fraksi etil asetat, dan fraksi air masing-masing dengan dosis 5,15 mg/kgBB. Metode yang digunakan adalah metode kantung granuloma (granuloma pouch). Udem pada tikus diinduksi dengan menyuntikkan karagenin 2% secara subkutan. Suspensi fraksi diberikan secara oral, satu jam sebelum induksi udem. Volume eksudat, jumlah leukosit, monosit, neutrofil, dan limfosit eksudat diukur setelah 24 jam. Data yang didapat diuji secara statistik dengan one-way ANOVA yang dilanjutkan dengan uji Tukey. Hasil penelitian menunjukkan bahwa fraksi etil asetat dengan dosis 5,15 mg/kgBB tikus dapat menurunkan volume eksudat dan jumlah leukosit eksudat secara signifikan (p<0,05). Efek antiinflamasi fraksi ini juga sebanding dengan kontrol positif yaitu natrium diklofenak dengan dosis 10,278 mg/kgBB tikus.
Anredera cordifolia [Ten] Steenis has been proven to have pharmacological activity that is thought to be influenced by the presence of phenolic compounds and flavonoids. However, both compounds are found in small concentrations through conventional extraction (maceration, decoction, soxhlet, and reflux). This study aims to prove that the ultrasonic assisted extraction method can obtain higher concentrations of phenolic and flavonoid compounds. Dried binahong leaves were extracted using ultrasonic assisted extraction with 70% ethanol. Phenolic levels were determined using Folin-Ciocalteu, while flavonoid levels were determined using the colorimetric aluminum method. The results showed binahong leaves ethanolic extract had phenolic levels of 10.16 % GAE and flavonoid levels of 10.58 % QE. These results are much higher than the results obtained using conventional extraction so that ultrasonic assisted extraction can be recommended to maximize the extraction of phenolic and flavonoid levels from the leaves of Binahong (Anredera cordifolia [Ten] Steenis).
Background: Arabica coffee leaf (Coffea arabica L.) is a well-known plant by the Indonesian people because it has many benefits apart from being a drink. Coffee leaves are known to have good antioxidant activity. The use of antioxidants in sunscreen preparations can prevent various diseases caused by UV radiation from UV rays’ sunlight. Aim: This study aims to determine the phenol content, the flavonoid content and the value of Sun Protection Factor (SPF) from differences between solvent extracts of arabica coffee leaves (Coffea arabica L.). Method: The extraction method used was maceration with solvents: 70% ethanol, 96% ethanol and methanol. Determination of phenol content was carried out using the Folin ciocalteu method. Determination of flavonoid content was done by the AlCl3 method. SPF value is based on the Mansur equation from absorbance scanning using UV Spectrophotometry. Result: The results obtained were the highest phenol and flavonoid content is in the methanolic extract of arabica coffee namely 62.371 ± 0.47 mgGAE/g and 8.6707 ± 0.04 mgQE/g. In the SPF test, the highest value was obtained from 70% ethanol extract of 5.0593 ± 1.28 at 100 ppm extract concentration with intermediate protection category. Conclusion: Arabica coffee leaves have the potential as an active ingredient in herbal sunscreens
Gel merupakan sistem semi padat terdiri dari suspensi yang dibuat dari partikel anorganik yang kecil atau molekul organik yang besar, terpenetrasi oleh suatu cairan. Gel membutuhkan basis yang bersifat polimer yaitu polimer alami, semi sintetik dan sintetik. Penambahan gelling agent dapat mempengaruhi sifat fisik sediaan gel, sehingga membutuhkan uji sifat fisik untuk mendapatkan sediaan yang baik. Tujuan penelitian ini untuk mengulas dan melihat jumlah penggunaan gelling agent polimer yang berpengaruh terhadap sifat fisik sediaan topikal gel yang baik. Metode penelitian yang digunakan dalam penelitian ini adalah Narrative Review. Berdasarkan hasil kajian literatur dan pembahasan ulasan artikel yang telah dibuat bahwa pada gelling agent yang lazim digunakan dan memiliki sifat fisik yang baik untuk sediaan topikal gel yaitu pada sintetik carbomer, polimer semi sintetik HPMC, Na.CMC dan HEC, dan pada polimer alami gelati, gellan gum, Na.Alginat dan xanthan gum. Kesimpulan penelitian ini bahwa bahwa jenis polimer alami merupakan gelling agent yang paling banyak digunakan untuk sediaan topikal gel. Berbagai jenis polimer tersebut diantaranya yang paling populer adalah gelatin, gellan gum, natrium alginat dan xanthan gum. Semua jenis polimer alami yang dipilih memberikan sifat fisik gel yang baik dan memenuhi persyaratan farmasetika.
Extract cincau leaves green according to previous studies have the value of the antioxidant IC50 49,45±0,64 µg/mL. According to researchers the phenol formerly produced through a method of drying oven with higher than the drying with sunlight and dried use wind. The research aims to understand the influence of means drying simplisia against phenolic levels total and the activity of a natural sunscreen on extract ethanol 70 % cincau leaves green (Cyclea barbata Miers). Drying simplisia in research is divided into three which is drying with the dry wind, the sun, and an oven. Green leaves cincau simplisia extracted by using the method maceration. Testing the phenolic committed using the folin ciocalteu method. The determination is based on the SPF value Mansur. The results of the value of phenolic or on the program have managed to extract by the difference means drying simplisia dry wind, the rays of the sun and an oven respectively namely 32,7089; 46,2500 and 59,5500 mgGAE / g. The SPF value obtained from each extract with different methods of drying simplisia showed significant differences in various concentration test.
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