Total Phenolic, Total Flavonoid, Quercetin Content and Antioxidant Activity of Standardized Extract of Moringa oleifera Leaf from Regions with Different Elevation

Sulastri, Evi; Zubair, Muhammad Sulaiman; Anas, Nurafni Israyanti; Abidin, Syakila; Hardani, Ririen; Yulianti, Risfah; Aliyah,

doi:10.5530/pj.2018.6s.20

Cited by 68 publications

(46 citation statements)

References 12 publications

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“…The physicochemical analysis is responsible for ensuring the quality and purity of Mikania micrantha 10 . The value of water content showed values less than 10 % that means sample will not allow for microbial growth.…”

Section: Discussionmentioning

confidence: 99%

Total Phenolic, Total Flavonoid and Phytochemical Screening by FTIR Spectroscopic of Standardized Extract of Mikania micrantha Leaf

Sumantri¹,

Wahyuni²,

Mustanti³

2020

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Mikania micrantha leaves were dried at room temperature and smashed manually. Each sample was extracted by maceration method with ethanol 96% at room temperature for 5 x 24 h. The liquid extract obtained was then evaporated with a rotary ABSTRACT Background: Mikania micrantha is a great plant that has been used as raw material for traditional medicines. Objective: This paper aims to evaluate total phenols, total flavonoids, and phytochemical screening by FTIR spectroscopy of standardized extract of Mikania micrantha leaf to confirm its medicinal values. Materials and Methods: The leaves were extracted by maceration method using ethanol 96% and evaporated by rotary evaporator. The determination of total phenolic and total flavonoid were performed by spectroscopic method. The phytochemical constituent was screened through the bioactive group of the chemical by FTIR analysis. Results: The total phenolic of extract of Mikania micrantha leaf ranged from 13.19±0.74 to 34.24±1.24 mg gallic acid equivalent/g and total flavonoid ranged from 1.11±0.11 to 20.63±0.16 mg quercetin/g. FTIR analysis confirmed the presence of O-H, aliphatic CH, and C=O functional group. Conclusion: the result of this study confirm that Mikania micrantha possesses the potential of bioactive compounds which are responsible for the biological activities that are useful for raw material of traditional medicines.

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Section: Discussionmentioning

confidence: 99%

Total Phenolic, Total Flavonoid and Phytochemical Screening by FTIR Spectroscopic of Standardized Extract of Mikania micrantha Leaf

Sumantri¹,

Wahyuni²,

Mustanti³

2020

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“…For quantitative analysis, total flavonoids and quercetin concentration were determined as our previous research. 14 The analysis of quercetin as a marker compound on ethanolic extract (after confirmation of its presence) was done by Reverse-Phase-High Performance Liquid Chromatography (RP-HPLC). Briefly, the series concentration of standard quercetin (1.2, 2.4 and 4.8 μg/mL) in methanol was prepared.…”

Section: Phytochemical Analysismentioning

confidence: 99%

Phytochemical Analysis and Antihyperuricemic Activity of Ethanolic Extract of Moringa oleifera Seeds

Ibrahim¹,

Nuryanti²,

Hasanuddin³

et al. 2020

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Background: Moringa oleifera is a popular plant that has been known to have several importance biological activities. Objectives: To perform phytochemical analysis, to in vivo evaluate the antihyperuricemic activity and to measure the inhibition of ethanolic extract of Moringa oleifera seed on xanthine oxidase enzyme. Materials and Methods: The seed were collected from Sigi regency, Central Sulawesi, Indonesia. Extraction was performed by maceration method with ethanol 96% as a solvent. Thin layer chromatography (TLC), high performance liquid chromatography (HPLC) and spectrophotometric UV-Vis were used to determine the phytochemical contents. The antihyperuricemic activity was evaluated by using in vivo model of rat induced by potassium oxonate. The xanthin oxidase inhibitory activity was also determined by spectroscopic method by measuring the catalytic rate of xanthin oxidase enzyme. Results: Phytochemical analysis confirmed the presence of alkaloids, terpenoids, flavonoids, phenolics, saponin and tannin. Quantitative determination of total flavonoids and quercetin concentration found the values of 82.17 ± 0.684 mg QE/g and 0.5131 ± 0.0022 mg/g dried extract, respectively. Ethanolic extract of Moringa oleifera seed have potential antihyperuricemic activity in which it can significantly reduce the serum uric acid level on potassium oxonate-induced hyperuricemic rat model with the effective dose of 125 mg/ kg BW. Xanthin oxidase inhibitory activity showed the moderate activity with IC 50 of 88.39 μg/ml. Conclusion: This study confirmed the potential of Moringa oleifera seed ethanolic extract, growing in Sigi, Central Sulawesi to be developed as herbal medicinal source for antihyperuricemic drug.

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“…Quercetin was used as a standard. The total flavonoid content ofthe sample was determined based onquercetin standard curve with various concentrations (50, 100, 150, 200, 250, and 300 μg/mL)and the result was in milligram per quercetin (mg/g QUE) that is the milligram of quercetin equivalent per 1 g extract [11] .…”

Section: Determination Of Total Flavonoidmentioning

confidence: 99%

Phenolic Profile and Antioksidant Activity of Trigona Honey in Bone, South Sulawesi, Indonesia

Tri Damayanty Syamsul,

Rosdiana Natzir,

Suryani As’ad

et al. 2021

MLU

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Introduction: Stingless bee honey has different characteristics from other types of bee honey in terms of colors, tastes, water content, and sugar. Trigona honey is one of the local products in South Sulawesi produced by stingless bees from Tetragonula species widely known to the public. This study determined the phenol content of trigona honey and its antioxidant activity. Method: The compounds of trigona honey ethanol extract were identified using gas chromatography. The antioxidant activity of the extract was examined with the DPPH method. Result Content test shows that trigona honey has bioactive compounds, flavonoids, alkaloids, and triterpenoids. DPPH show to trigona honey 104,25. Conclusion: Trigona honey contains mild antioxidant activity and metabolite compounds such as polyphenols, flavonoids, alkaloids, and triterpenoids. The nutrient activity can reduce blood glucose and increase insulin plasma.

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Total Phenolic, Total Flavonoid, Quercetin Content and Antioxidant Activity of Standardized Extract of Moringa oleifera Leaf from Regions with Different Elevation

Cited by 68 publications

References 12 publications

Total Phenolic, Total Flavonoid and Phytochemical Screening by FTIR Spectroscopic of Standardized Extract of Mikania micrantha Leaf

Total Phenolic, Total Flavonoid and Phytochemical Screening by FTIR Spectroscopic of Standardized Extract of Mikania micrantha Leaf

Phytochemical Analysis and Antihyperuricemic Activity of Ethanolic Extract of Moringa oleifera Seeds

Phenolic Profile and Antioksidant Activity of Trigona Honey in Bone, South Sulawesi, Indonesia

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