Total Phenolic, Total Flavonoid, Tannin Content, and Antioxidant Capacity of Halimium halimifolium (Cistaceae)

Abstract

This study was carried out to evaluate the phytochemical constituents and antioxidant potential of the leaves and flowers extracts of Halimium halimifolium in order to validate the medicinal potential of this herb. The antioxidant activity of alcoholic and aqueous extracts was evaluated using 2, 2-diphenyl-l-picrylhydrazyl (DPPH), 2, 2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and ferric reducing antioxidant power (FRAP) assays. The total polyphenol, flavonoid and tannin content were determined according respectively to Folin-Ciocalteu method, Zhishen method and Broadhurst method. The leaves of H. halimifolium had greater antioxidant activity than flowers by DPPH and ABTS assays. The ethanol extract of the leaves exhibited the better antioxidant activity by DPPH assay (IC50 = 1.19 µg.mL-1), the n-butanol by ABTS assays (IC50 = 1.73µg.mL-1). As opposed to FRAP method the flowers had greater antioxidant activity as leaves. Ethyl acetate extract exhibited better antioxidant activity (IC50 = 15.5 mmol Fe 2+ .g-1). The obtained results showed that the extracting solvent significantly influenced the antioxidant property estimations of Halimium halimifolium. The ethanol is a recommended solvent for extracting antioxidants from this plant. The antioxidant activity determined by DPPH, ABTS and FRAP demonstrated a linear relationship with their polyphenols, flavonoids and tannins content.

Antioxidant and Antiulcerogenic Activity of the Dry Extract of Pods of Libidibia ferrea Mart. ex Tul. (Fabaceae)

“…Studies indicate that there is no single method capable of quantitatively and accurately evaluating antioxidant properties. As the various methodologies differ in their mechanisms of action, they are considered complementary in the study of the antioxidant potential of plants [74, 75].…”

Antioxidant Capacity and Antigenotoxic Effect of Hibiscus sabdariffa L. Extracts Obtained with Ultrasound-Assisted Extraction Process

“…Many and diverse compounds have been identified in Roselle with biological properties. It has been noted that flavonoids are the main antioxidant group [42]. Quercetin, myricetin and proanthocyanidins, of the epigallocatechin and epigallocatechin-gallate type catechin, are flavonoids found in H. sabdariffa [43].…”

The Effect of Capsella bursa-pastoris Extract on Heavy Menstrual Bleeding and Quality of Life in Patients with Uterine Leiomyoma: A Double-blind Randomized Clinical Trial

“…In the past, tyramine and choline were responsible for controlling the bleeding by the plant. Todays, new researches have shown that other substances that are not fully recognized may be responsible for such an effect [25][26][27][28][29][30][31][32][33][34][35][36][37][38]. Anyway, the contractile and hemostatic nature of this drug cannot be ignored.…”

“…The average therapeutic dose of CBP for internally use is 10-15 grams dry plant per day [21,29]. The extract of CBP was analyzed for the total Phenolic, flavonoid, and tannin content, respectively by the Folin-Ciocalteu colorimetric method, aluminium trichloride method and titrimetric indigosulphonic acid assay [30][31][32][33].…”