Touch Imprint Cytology in Biopsy of the Infertile Testis

Düşmez, Duygu; Aydın, Özlem; Akbay, Erdem; Çayan, Selahíttín; Kanik, Emine Arzu

doi:10.1159/000328376

Cited by 5 publications

(4 citation statements)

References 9 publications

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“…In our study group, the age was in the range of 22-48 years, and the duration of infertility was > 2 years. This is similar to the findings of Batra et al [11], Rammou-Kinia et al [12], Düşmez et al [13], Kurien et al [14], and Agarwal et al [15], who all found the age group of 20-50 years to be the most commonly involved. The reason for this could be the settling of couples into family life in this period.…”

Section: Discussionsupporting

confidence: 91%

Fine-Needle Aspiration Cytology of the Testes for the Classification of Azoospermia and Its Value in the Assessment of Male Infertility

et al. 2019

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Background: Infertility is an ever-increasing problem in today's world. It can be due to male or female causes. Azoospermia seen in 5-10% of infertile men is due to obstructive or non-obstructive causes. Traditionally, testicular biopsy is the gold standard for evaluation. Fine-needle aspiration (FNA), however, is minimally invasive, provides qualitative and quantitative information about spermatogenesis, and can aid in assisted reproductive techniques making it a novel technique for the evaluation of male infertility. Objective: We aimed to classify different causes of azoospermia into different patterns based upon FNA, and assess the utility of cell indices in classifying cases into different patterns. Method: We conducted a prospective and a retrospective study of 42 azoospermic males, confirmed on semen analysis, over a period of 5 years. Patients were subjected to FNA of the testes. Smears were prepared, air-dried, wet-fixed, and then stained with May-Grünwald Giemsa and Papanicolaou stains, respectively. Cells were identified using predetermined morphologic criteria, and various indices were calculated followed by statistical analysis of the observations. Re-sults: The mean age of 40 patients who satisfied the adequacy criteria was 32.75 years (range 22-48 years). Thirty-four patients had primary infertility and 6 had secondary infertility. Of these, 12 had normal spermatogenesis, 8 had hypospermatogenesis, 3 had early and 7 had late maturation arrest, 6 had Sertoli cell-only syndrome (SCOS), and there were different results in each testicle in 4 cases. The Sperm Index (SI) was significantly higher in all cases of normal spermatogenesis than in any of the hypo-spermatogenesis cases (p = 0.009). The Sertoli Index (SEI) in cases of hypo-spermatogenesis and maturation arrest was significantly higher than in cases of normal spermatogenesis (p < 0.001). The Sperm-Sertoli Index (SSI) also showed significant differences between cases of hypo-spermatogenesis and normal spermatogenesis (p < 0.001). These indices were useful in categorising patients with azoospermia. Conclusion: FNA helps to easily and accurately identify all types of testicular cells without biopsy. SI, SEI, and SSI are powerful cell indices for assessing the extent of spermatogenesis and classifying various causes of azoospermia. Bilateral sampling and multiple aspirations give a better mapping of spermatogenesis within the testes. Testicular FNA can thus play a very important role in the evaluation of male infertility.

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Section: Discussionsupporting

confidence: 91%

Fine-Needle Aspiration Cytology of the Testes for the Classification of Azoospermia and Its Value in the Assessment of Male Infertility

et al. 2019

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“…A touch imprint technique has been described as an adjunct of testicular biopsy for the immediate interpretation of testicular cytological findings (Abdelmoneim et al, 2004). Moreover, it has been established that testicular cytology (using either TFNA or imprints) can be more precised than histologic surgical biopsy to assess the spermatogenic status of the testis (Düsmez et al, 2001;Amin et al, 2006). Percentages from spermatogonia to late spermatids were increased showing a normal evolution of the spermatogenic process in both smears.…”

Section: Discussionmentioning

confidence: 99%

Testicular cytology of alpaca: Comparison between impressed and smeared slides

Stelletta

Juyena

Ponce-Salazar

et al. 2011

Animal Reproduction Science

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“…Of all cytological techniques imprint cytology is the closest to pathology and has been found to be useful, quick, reliable and popular in the diagnosis of solid tumours 7–12 . In the upper respiratory tract, imprint cytology has been used only for the investigation of some aspects of Reinke's oedema 13 .…”

Section: Introductionmentioning

confidence: 99%

“…[3][4][5][6] Of all cytological techniques imprint cytology is the closest to pathology and has been found to be useful, quick, reliable and popular in the diagnosis of solid tumours. [7][8][9][10][11][12] In the upper respiratory tract, imprint cytology has been used only for the investigation of some aspects of Reinke's oedema. 13 We introduced touch imprint cytology to test its reliability and accuracy in preoperative management in patients with pharyngeal and laryngeal tumours, as well as the possible application of this method in diagnosis during operation.…”

Section: Introductionmentioning

confidence: 99%