Tough and elastic hydrogel of hyaluronic acid and chondroitin sulfate as potential cell scaffold materials

Ni, Yilu; Tang, Zhurong; Cao, Wanxu; Lin, Hai; Fan, Yujiang; Guo, Likun; Zhang, Xingdong

doi:10.1016/j.ijbiomac.2014.10.058

Cited by 61 publications

(23 citation statements)

References 39 publications

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“…Further, collagen can be mixed with GAGs to generate artificial ECM and subsequently used for material coatings, facilitated by the fibrillogenesis of collagen (Schnabelrauch et al, 2013). Another application, especially of multilayer-assembled or -coated microcapsules/-beads, represents the transport of drugs, since the association with specific binding molecules such as GAGs can prevent drugs from rapid release and (Cai et al, 2005;Elia et al, 2013;Shu et al, 2006) Cross-linked CS-tyramine Hydrogel Drug delivery (Jin et al, 2013;Ni et al, 2015) Cross-linked aldehyde HA Hydrogel Vocal cord/ fold regeneration (Jia et al, 2006;Thibeault et al, 2011) Gelatinaldehyde Hep + FGF-2…”

Section: Immobilization Of Glycosaminoglycans On Implants Scaffolds mentioning

confidence: 99%

Medical application of glycosaminoglycans: a review

Köwitsch

Zhou

Groth

2017

J Tissue Eng Regen Med

182

145

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The characteristic molecular composition of the different glycosaminoglycans (GAGs) is related to their role as structural components and regulators of a multitude of functions of proteins, cells and tissues in the human body. Therefore, it is not surprising that GAGs are widely used as coating materials for implants, components of 3D-constructs such as tissue engineering scaffolds and hydrogels, but also as diagnostic devices such as biosensors and in controlled release applications. Beside a physisorption or encapsulation of GAGs, these applications often require their chemical modification to allow a stable covalent attachment on surfaces or cross-linking reactions with other molecules. Then, the preservation of the functionality of GAGs under maintenance of their biocompatibility is a challenging task and must be addressed in accordance with the designated field of application. Here, we will give a brief overview on structure and biological functions of GAGs, different methods of their activation and immobilization, the recent progress in GAG-related biomaterials development, as well as some examples of their application in the field of tissue engineering and regenerative medicine.

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Section: Immobilization Of Glycosaminoglycans On Implants Scaffolds mentioning

confidence: 99%

Medical application of glycosaminoglycans: a review

Köwitsch

Zhou

Groth

2017

J Tissue Eng Regen Med

182

145

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“…HA coating could play additional beneficial effects on cell therapy because HA can stimulate the expression of adhesion molecules, thus favoring the proliferation and engraftment of hBTSCs. Previous studies highlighted varied HA trophic effects in different cell systems [12, 13, 20, 21, 38, 42]. HA plays a key role in stabilizing and organizing the ECM by adjusting the adhesion and cell motility, mediating proliferation and differentiation as exemplified in the following examples.…”

Section: Discussionmentioning

confidence: 99%

“…HA plays a key role in stabilizing and organizing the ECM by adjusting the adhesion and cell motility, mediating proliferation and differentiation as exemplified in the following examples. The authors demonstrated paracrine interactions between the implanted cells, the host ECM, and endothelial cells, and a proangiogenic effect of degradation of exogenous HA [12, 13, 20, 21, 38, 42]. …”

Section: Discussionmentioning

confidence: 99%

“…We hypothesize that this strategy could be successful for the liver given that HA is selectively and specifically cleared by the liver [22, 23]. Moreover, HAs are largely used for different clinical applications, facilitating their use also in the setting of cell therapy [12]. The main objectives of the present study were to evaluate the effect of HA coating: in vitro, on engraftment, proliferation, and differentiation of hBTSCs in primary cultures; and in vivo, on engraftment efficiency into the liver and differentiation toward mature hepatocytes of hBTSCs transplanted, via the spleen, into immune-compromised mice.…”

Section: Introductionmentioning

confidence: 99%

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Hyaluronan coating improves liver engraftment of transplanted human biliary tree stem/progenitor cells

et al. 2017

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BackgroundCell therapy of liver diseases with human biliary tree stem cells (hBTSCs) is biased by low engraftment efficiency. Coating the hBTSCs with hyaluronans (HAs), the primary constituents of all stem cell niches, could facilitate cell survival, proliferation, and, specifically, liver engraftment given that HAs are cleared selectively by the liver.MethodsWe developed a fast and easy method to coat hBTSCs with HA and assessed the effects of HA-coating on cell properties in vitro and in vivo.ResultsThe HA coating markedly improved the viability, colony formation, and population doubling of hBTSCs in primary cultures, and resulted in a higher expression of integrins that mediate cell attachment to matrix components. When HA-coated hBTSCs were transplanted via the spleen into the liver of immunocompromised mice, the engraftment efficiency increased to 11% with respect to 3% of uncoated cells. Notably, HA-coated hBTSC transplantation in mice resulted in a 10-fold increase of human albumin gene expression in the liver and in a 2-fold increase of human albumin serum levels with respect to uncoated cells. Studies in distant organs showed minimal ectopic cell distribution without differences between HA-coated and uncoated hBTSCs and, specifically, cell seeding in the kidney was excluded.ConclusionsA ready and economical procedure of HA cell coating greatly enhanced the liver engraftment of transplanted hBTSCs and improved their differentiation toward mature hepatocytes. HA coating could improve outcomes of stem cell therapies of liver diseases and could be immediately translated into the clinic given that GMP-grade HAs are already available for clinical use.Electronic supplementary materialThe online version of this article (doi:10.1186/s13287-017-0492-7) contains supplementary material, which is available to authorized users.

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“…However, the in vivo application of scaffolds made of synthetic polymers, e.g., poly(glycolic acid), can be limited due to their toxic degradation products . Nevertheless, this negative effect can be avoided by using natural materials for hydrogel preparation, such as agarose, alginate, chitosan, chondroitin sulfate, collagen, dextran, fibrin, gelatin, hyaluronic acid, matrigel, and silk …”

Section: Introductionmentioning

confidence: 99%

Generation of Large‐Scale DNA Hydrogels with Excellent Blood and Cell Compatibility

Stoll¹,

Steinle²,

Stang³

et al. 2016

Macromolecular Bioscience

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Hemocompatibility and cytocompatibility of biomaterials codetermine the success of tissue engineering applications. DNA, the natural component of our cells, is an auspicious biomaterial for the generation of designable scaffolds with tailorable characteristics. In this study, a combination of rolling circle amplification and multiprimed chain amplification is used to generate hydrogels at centimeter scale consisting solely of DNA. Using an in vitro rotation model and fresh human blood, the reaction of the hemostatic system on DNA hydrogels is analyzed. The measurements of hemolysis, platelets activation, and the activation of the complement, coagulation, and neutrophils using enzyme-linked immunosorbent assays demonstrate excellent hemocompatibility. In addition, the cytocompatibility of the DNA hydrogels is tested by indirect contact (agar diffusion tests) and material extract experiments with L929 murine fibroblasts according to the ISO 10993-5 specifications and no negative impact on the cell viability is detected. These results indicate the promising potential of DNA hydrogels as biomaterials for versatile applications in the field of regenerative medicine.

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Tough and elastic hydrogel of hyaluronic acid and chondroitin sulfate as potential cell scaffold materials

Cited by 61 publications

References 39 publications

Medical application of glycosaminoglycans: a review

Medical application of glycosaminoglycans: a review

Hyaluronan coating improves liver engraftment of transplanted human biliary tree stem/progenitor cells

Generation of Large‐Scale DNA Hydrogels with Excellent Blood and Cell Compatibility

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