Tourism, a significant industry worldwide, has allowed for economic, social, and cultural development in rural areas because of all the advantages it offers to tourists. Ecuador is a country that presents a wide range of alternatives in the tourist field allowing a massive tourist influx to many of its destinations such as indigenous communities, natural reserves, and tourist, ecological, and volcanic routes. Most importantly, Ecuador encourages the conservation of the diversity resulting from such influx. The objective of this research is to design an ontology which facilitates a description of the bicycle tourist routes in the province of Tungurahua, and specifically in the city of Ambato. Apart from route descriptions, this ontology gives information about the flora and fauna and places of interest such as inns, hotels, and restaurants that can be found along this route. This ontology was developed based on the application and enforcement of semantic web technologies. That being said, all the described information is attached to an RDF vocabulary and it can be accessed using SPARQL. Therefore, information that is semantically described in an organized and standardized manner is available in order to allow for interoperability with other existing data sources. As for the development of the ontology, the methodology used was an open code software known as “protégé”. The OWL (Ontology Web Language) was also used as marked language and RDFS for the description of the employed vocabulary.