Toward A Highly Specific DNA Biosensor: PNA-Modified Suspended-Core Photonic Crystal Fibers

Coscelli, Enrico; Sozzi, Michele; Poli, Federica; Passaro, D.; Cucinotta, Annamaria; Selleri, Stefano; Corradini, Roberto; Marchelli, Rosangela

doi:10.1109/jstqe.2009.2031923

Cited by 75 publications

(39 citation statements)

References 24 publications

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“…Nevertheless, direct comparison between the solutionsolution and solution-surface cases is generally inappropriate due to differences in their kinetics [17]. Another factor affecting the fluorescence results is that in the relatively large core SCF used in this work, a relatively small proportion of the guided field is located in the voids compared to other reported SCFs [14] and will not excite the fluorophore of the MB if it is located too far from the surface. That is, a significant proportion of the MBs may not be effectively excited in this experiment.…”

Section: Resultsmentioning

confidence: 97%

“…Solutions under examination can be loaded into the air holes of the SCFs for direct interaction with this portion of the guided light, leading to the potential for high sensitivity [10]. Recently, SCFs have also been used for DNA sensing by immobilizing a PNA probe on the core of a SCF for specific DNA detection using the high selectivity of PNA for its cDNA sequence [14]. However, this approach still requires the cDNA to be labeled before detection, and the work does not extend to the detection of DNA using the PNAimmobilized SCFs; instead a characterization of PNA coating upon filling the fiber with DNA solutions using fluorescence imaging of the SCFs with excitation light incident on the fiber side rather than being coupled in the suspended core was reported [14].…”

Section: Introductionmentioning

confidence: 99%

“…Recently, SCFs have also been used for DNA sensing by immobilizing a PNA probe on the core of a SCF for specific DNA detection using the high selectivity of PNA for its cDNA sequence [14]. However, this approach still requires the cDNA to be labeled before detection, and the work does not extend to the detection of DNA using the PNAimmobilized SCFs; instead a characterization of PNA coating upon filling the fiber with DNA solutions using fluorescence imaging of the SCFs with excitation light incident on the fiber side rather than being coupled in the suspended core was reported [14]. While SCFs have been demonstrated to be a promising candidate for biosensors, particularly those based on fluorescence approaches, to the best of our knowledge there has been no report to date on DNA detection through SCFs or MOFs immobilized with a specific DNA probe on the surface of the fiber core.…”

Section: Introductionmentioning

confidence: 99%

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Molecular beacons immobilized within suspended core optical fiber for specific DNA detection

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Section: Resultsmentioning

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Molecular beacons immobilized within suspended core optical fiber for specific DNA detection

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“…Fiber-based fluorescence sensing has been deployed in sensing targets such as: aluminum ions [7], hydrogen peroxide [8], oxygen [9], and explosives [10]. Optical fiber based fluorescence sensors have also been deployed for biological applications, including measurements of biomolecules in solution [11], DNA [12], or enzyme assays within microstructured fibers [13].…”

Section: Introductionmentioning

confidence: 99%

Quantification of the fluorescence sensing performance of microstructured optical fibers compared to multi-mode fiber tips

et al. 2016

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Rights url: https://www.osapublishing.org/submit/review/copyright_permissions.cfm# Creative Commons LicensingOSA is aware that some authors, as a condition of their funding, must publish their work under a Creative Commons license. We therefore offer a CC BY license for authors who indicate that their work is funded by agencies that we have confirmed have this requirement. Authors must enter their funder(s) during the manuscript submission process. At that point, if appropriate, the CC BY license option will be available to select for an additional fee.Any subsequent reuse or distribution of content licensed under CC BY must maintain attribution to the author(s) and the published article's title, journal citation, and DOI. Abstract: Microstructured optical fibers, particularly those with a suspended-core geometry, have frequently been argued as efficient evanescent-field fluorescence-based sensors. However, to date there has not been a systematic comparison between such fibers and the more common geometry of a multi-mode fiber tip sensor. In this paper we make a direct comparison between these two fiber sensor geometries both theoretically and experimentally.Our results confirm that suspended-core fibers provide a significant advantage in terms of total collected fluorescence signal compared to multi-mode fibers using an equivalent experimental configuration. Noordegraaf, K. Nielsen, A. Carlsen, and A. Bjarklev, "Photonic crystal fiber based evanescent-wave sensor for detection of biomolecules in aqueous solutions," Opt. Lett. 29(17), 1974-1976 (2004). 12. E. Coscelli, M. Sozzi, F. Poli, D. Passaro, A. Cucinotta, S. Selleri, R. Corradini, and R. Marchelli, "Toward a highly specific DNA biosensor: Pna-modified suspended-core photonic crystal fibers," IEEE J. Sel. Top. Quantum Electron. 16(4), 967-972 (2010). 13. S. C. Warren-Smith, G. Nie, E. P. Schartner, L. A. Salamonsen, and T. M. Monro, "Enzyme activity assays within microstructured optical fibers enabled by automated alignment," Biomed. Opt. Express 3(12), 3304-3313 (2012). Opt. Quantum Electron. 26(3), S261-S272 (1994). 17. C. Bariain, I. R. Matías, F. J. Arregui, and M. Lopez-Amo, "Optical fiber humidity sensor based on a tapered fiber coated with agarose gel," Sensor. Actuat. Biol. Chem. 69, 127-131 (2000). 18. L. C. Shriver-Lake, K. A. Breslin, P. T. Charles, D. W. Conrad, J. P. Golden, and F. S. Ligler, "Detection of tnt in water using an evanescent wave fiber-optic biosensor," Anal. Chem. 67(14), 2431-2435 (1995)

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“…The SCFs can be made of a single material, in such case silica, and use for strain and temperature sensing based in a Sagnac and Fabry-Perot interferometer [5]. More recently, opening one of the holes of the MOF was exploited to maximize the interaction of light for evanescent field sensing or for bio-chemical application [6].…”

Section: Introductionmentioning

confidence: 99%

Temperature-independent pressure sensor using triangular-shape of suspended-core fiber

et al. 2012

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In this work, fiber in-line Mach-Zehnder Interferometer (MZI) based on triangular-shape suspended core fibers (SCFs) is investigated. The sensitivity of the sensing head was determined for pressure and temperature, respectively. The sensitivities are 0.4 pm/psi and 13 pm/psi for longitudinal and radial pressure, respectively. The sensing head was also subjected to temperature and presented very low sensitivity.

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Toward A Highly Specific DNA Biosensor: PNA-Modified Suspended-Core Photonic Crystal Fibers

Cited by 75 publications

References 24 publications

Molecular beacons immobilized within suspended core optical fiber for specific DNA detection

Molecular beacons immobilized within suspended core optical fiber for specific DNA detection

Quantification of the fluorescence sensing performance of microstructured optical fibers compared to multi-mode fiber tips

Temperature-independent pressure sensor using triangular-shape of suspended-core fiber

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