Toward assembling a taxonomic puzzle: Case study of Iranian gray voles of the subgenus Microtus (Rodentia, Cricetidae)

Golenishchev, Fedor N.; Malikov, Vladimir; Petrova, Tatyana V.; Bodrov, Semen; Abramson, Natalia I.

doi:10.1016/j.mambio.2018.06.007

Cited by 15 publications

(19 citation statements)

References 18 publications

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“…In the past, the taxonomy of Microtus was mainly based on morphological differentiation, distinct karyotypes and different chromosome numbers [39,40]. However, some species have similar chromosome number and karyotype but appear morphologically different, while some are opposite [41,42]. Therefore, this genus clearly requires revision to fit phylogenetic relationships indicated by molecular data [10,11,37,43,44].…”

Section: Discussionmentioning

confidence: 99%

“…The molecular systematic analysis of Microtus based on molecular data including both mitochondrial and nuclear markers [49,50]. In the past decades, most phylogenetic studies focused on mt genomes and some species received a good distinction [41,51]. Recently, some researchers are beginning to use both mtDNA and nuclear DNA to analyze the phylogeny of genus Microtus and obtain good results [52–54].…”

Section: Discussionmentioning

confidence: 99%

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Complete mitochondrial genomes confirm the generic placement of the plateau vole,Neodon fuscus

et al. 2019

Bioscience Reports

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The plateau vole, Neodon fuscus is endemic to China and is distributed mainly in Qinghai Province. It is of public health interest, as it is, a potential reservoir of Toxoplasma gondii and the intermediate host of Echinococcus multilocularis. However, genetic data of this species are lacking, and its name and taxonomy are still a controversy. In the present study, we determined the nucleotide sequence of the entire mitochondrial (mt) genome of N. fuscus and analyzed its evolutionary relationship. The mitogenome was 16328 bp in length and contained 13 protein-coding genes, 22 genes for transfer RNAs (tRNA), two ribosomal RNA genes and two major noncoding regions (OL region and D-loop region). Most genes were located on the heavy strand. All tRNA genes had typical cloverleaf structures except for tRNASer (GCU). The mt genome of N. fuscus was rich in A+T (58.45%). Maximum likelihood (ML) and Bayesian methods yielded phylogenetic trees from 33 mt genomes of Arvicolinae, in which N. fuscus formed a sister group with Neodon irene and Neodon sikimensis to the exclusion of species of Microtus and other members of the Arvicolinae. Further phylogenetic analyses (ML only) based on the cytb gene sequences also demonstrated that N. fuscus had a close relationship with N. irene. The complete mitochondrial genome was successfully assembled and annotated, providing the necessary information for the phylogenetic analyses. Although the name Lasiopodomys fuscus was used in the book ‘Wilson & Reeder’s Mammal Species of the World’, we have confirmed here that its appropriate name is N. fuscus through an analysis of the evolutionary relationships.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Complete mitochondrial genomes confirm the generic placement of the plateau vole,Neodon fuscus

et al. 2019

Bioscience Reports

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“…All species examined showed intrinsic postzygotic isolation in the form of hybrid sterility. This sterility is more advanced between M. kermanensis × M. arvalis and (Mahmoudi et al, 2017;Golenishchev et al, 2019); additionally, M. kermanensis does not share at least 14 chromosome rearrangements with M. arvalis and at least eight with M. transcaspicus (Mazurok et al, 2001).…”

Section: Species In the "Mystacinus" Group Had Already Evolved Male Hybrid Sterilitymentioning

confidence: 99%

“…According to a recent revision ( Shenbrot and Krasnov, 2005 ; Pavlinov and Lissovsky, 2012 ; Golenishchev et al, 2019 ), the subgenus Microtus comprises six nominal forms: M. arvalis (Pallas, 1778) (with two karyoforms, “arvalis” and “obscurus”), M. rossiaemeridionalis (Ognev, 1924), M. ilaeus (Thomas, 1912), M. transcaspicus (Satunin, 1905), Microtus kermanensis (Roguin, 1988), and Microtus mystacinus (De Filippi, 1865). The former two sibling species occupy vast areas in Eurasia, cohabitating in most of them.…”

Section: Introductionmentioning

confidence: 99%

“…However, Mahmoudi et al (2014) suggested that, based on chromosome analysis, this form should be considered a valid name and showed its close affinity to the East European sibling vole M. rossiaemeridionalis ( Mahmoudi et al, 2017 ). Later on, a comparative analysis of the cytb gene indicated that M. kermanensis and M. mystacinus form a clade separate from M. rossiaemeridionalis ( Golenishchev et al, 2019 ). Figure 1 shows a consensus tree of the subgenus.…”

Section: Introductionmentioning

confidence: 99%

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Reproductive Isolation Between Taxonomically Controversial Forms of the Gray Voles (Microtus, Rodentia; Arvicolinae): Cytological Mechanisms and Taxonomical Implications

et al. 2021

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The formation of hybrid sterility is an important stage of speciation. The voles of the genus Microtus, which is the most speciose genus of rodents, provide a good model for studying the cytological mechanisms of hybrid sterility. The voles of the “mystacinus” group of the subgenus Microtus (2n = 54) comprising several recently diverged forms with unclear taxonomic status are especially interesting. To resolve the taxonomic status of Microtus mystacinus and Microtus kermanensis, we crossed both with Microtus rossiaemeridionalis, and M. kermanensis alone with Microtus arvalis “obscurus” and M. transcaspicus and examined the reproductive performance of their F1 hybrids. All interspecies male hybrids were sterile. Female M. kermanensis × M. arvalis and M. kermanensis × M. transcaspicus hybrids were sterile as well. Therefore, M. mystacinus, M. kermanensis, and M. rossiaemeridionalis could be considered valid species. To gain an insight into the cytological mechanisms of male hybrid sterility, we carried out a histological analysis of spermatogenesis and a cytological analysis of chromosome synapsis, recombination, and epigenetic chromatin modifications in the germ cells of the hybrids using immunolocalization of key meiotic proteins. The hybrids showed wide variation in the onset of spermatogenesis arrest stage, from mature (although abnormal) spermatozoa to spermatogonia only. Chromosome asynapsis was apparently the main cause of meiotic arrest. The degree of asynapsis varied widely across cells, individuals, and the crosses—from partial asynapsis of several small bivalents to complete asynapsis of all chromosomes. The asynapsis was accompanied by a delayed repair of DNA double-strand breaks marked by RAD51 antibodies and silencing of unpaired chromatin marked by γH2A.X antibodies. Overall, the severity of disturbances in spermatogenesis in general and in chromosome synapsis in particular increased in the hybrids with an increase in the phylogenetic distance between their parental species.

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The Tien Shan vole (Microtus ilaeus; Rodentia: Cricetidae) as a new species in the Late Pleistocene of Europe

Baca

Popović

Lemanik

et al. 2021

Ecology and Evolution

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Toward assembling a taxonomic puzzle: Case study of Iranian gray voles of the subgenus Microtus (Rodentia, Cricetidae)

Cited by 15 publications

References 18 publications

Complete mitochondrial genomes confirm the generic placement of the plateau vole,Neodon fuscus

Complete mitochondrial genomes confirm the generic placement of the plateau vole,Neodon fuscus

Reproductive Isolation Between Taxonomically Controversial Forms of the Gray Voles (Microtus, Rodentia; Arvicolinae): Cytological Mechanisms and Taxonomical Implications

The Tien Shan vole (Microtus ilaeus; Rodentia: Cricetidae) as a new species in the Late Pleistocene of Europe

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