Toward Marker Assisted Selection for Fungal Disease Resistance in Grapevine

Hvarleva, T.; Bakalova, A.; Rusanov, Krasimir; Diakova, G.; Ilieva, Irina; Atanassov, A.; Atanassov, Ilian

doi:10.2478/v10133-009-0008-4

Cited by 9 publications

(1 citation statement)

References 33 publications

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“…The completion of Vitis vinifera genome sequencing in a highly homozygous genotype and in a heterozygous grapevine variety has led to the identification of putative resistance genes and defense signaling elements (Jaillon et al, 2007;Casagrande et al, 2011). With the introduction of deep sequencing, a complex gene family encoding NBS-LRR proteins has been isolated and characterized in grapevine (Kortekamp et al, 2008;Hvarleva et al, 2009;Seehalak et al, 2011). However, functional characterization of Vitis RGAs remains unclear.…”

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confidence: 99%

Characterization and induction kinetics of a putative candidate gene associated with downy mildew resistance in grapevine

Liang¹,

Liu²,

Zang³

et al. 2015

Europ.J.Hort.Sci.

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Downy mildew in cultivated grapevines (Vitis spp.), caused by Plasmopara viticola, is a devastating disease that results in considerable economic losses as well as environmental damage due to the repeated application of fungicides. The molecular role of the NBS-LRR family is highly related to plant immune-activity against various pathogens and pests. In this study, the 5′ and 3′ ends of the resistance gene homology fragment, designated as RGA23, were obtained by rapid amplification of cDNA ends PCR (RACE-PCR), and a 2,789 bp full-length cDNA was obtained using the gene-specific primers based on the spliced sequence. The deduced 892 amino acid sequence of this cDNA contains a characteristic NBS-LRR domain of plant resistance genes and a coiled-coil (CC) region. We analyzed the expression of RGA23 under P. viticola infection and abiotic stress at different time points using real-time quantitative polymerase chain reaction (RT-qPCR). The results showed that P. viticola treatment and four tested abiotic stimuli, including SA, MeJA, ABA, and H 2 O 2 , triggered significant inductions of RGA23 within 12 d of inoculation. The results indicate that RGA23 may play a critical role in protecting grapevines against P. viticola through a signaling pathway triggered by P. viticola and these molecules.

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confidence: 99%

Characterization and induction kinetics of a putative candidate gene associated with downy mildew resistance in grapevine

Liang¹,

Liu²,

Zang³

et al. 2015

Europ.J.Hort.Sci.

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Development of Abiotic Stress Resistant Grapevine Varieties

Singh

Pradhan

KRISHNA

et al. 2022

Genomic Designing for Abiotic Stress Resistant Fruit Crops

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Rpv10: a new locus from the Asian Vitis gene pool for pyramiding downy mildew resistance loci in grapevine

Schwander¹,

Eibach²,

Fechter³

et al. 2011

Theor Appl Genet

176

172

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A population derived from a cross between grapevine breeding strain Gf.Ga-52-42 and cultivar 'Solaris' consisting of 265 F1-individuals was genetically mapped using SSR markers and screened for downy mildew resistance. Quantitative trait locus (QTL) analysis revealed two strong QTLs on linkage groups (LGs) 18 and 09. The locus on LG 18 was found to be identical with the previously described locus Rpv3 and is transmitted by Gf.Ga-52-42. 'Solaris' transmitted the resistance-related locus on LG 09 explaining up to 50% of the phenotypic variation in the population. This downy mildew resistance locus is named Rpv10 for resistance to Plasmopara viticola. Rpv10 was initially introgressed from Vitis amurensis, a wild species of the Asian Vitis gene pool. The one-LOD supported confidence interval of the QTL spans a section of 2.1 centi Morgan (cM) corresponding to 314 kb in the reference genome PN40024 (12x). Eight resistance gene analogues (RGAs) of the NBS-LRR type and additional resistance-linked genes are located in this region of PN40024. The F1 sub-population which contains the Rpv3 as well as the Rpv10 locus showed a significantly higher degree of resistance, indicating additive effects by pyramiding of resistance loci. Possibilities for using the resistance locus Rpv10 in a grapevine breeding programme are discussed. Furthermore, the marker data revealed 'Severnyi' × 'Muscat Ottonel' as the true parentage for the male parent of 'Solaris'.

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Toward Marker Assisted Selection for Fungal Disease Resistance in Grapevine

Cited by 9 publications

References 33 publications

Characterization and induction kinetics of a putative candidate gene associated with downy mildew resistance in grapevine

Characterization and induction kinetics of a putative candidate gene associated with downy mildew resistance in grapevine

Development of Abiotic Stress Resistant Grapevine Varieties

Rpv10: a new locus from the Asian Vitis gene pool for pyramiding downy mildew resistance loci in grapevine

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