Towards a better understanding of Pseudomonas putida biofilm formation in the presence of ZnO nanoparticles (NPs): Role of NP concentration

Ouyang, Kai; Mortimer, Monika; Holden, Patricia A.; Cai, Peng; Wu, Yichao; Gao, Chunhui; Huang, Qiaoyun

doi:10.1016/j.envint.2020.105485

Cited by 61 publications

(27 citation statements)

References 77 publications

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“…Similar to the results shown by Maurer-Jones et al [ 24 ], we also observed that the photocatalytic nanomaterial may affect the biofilm formation and physiology of bacteria. Moreover, Ouyang et al [ 40 ] showed that, on ZnO nanoparticles, sublethal concentrations may stimulate bacteria toward the secretion of biofilm-related signaling molecules. However, in our study, the biomass of biofilms was not affected in the majority of cases, while its respiration was highly upregulated after 48 h. The comparison to the control samples indicated that the presence of the nanomaterials in the growth environment could contribute to the aggregation of cells, although the material itself was not agglomerating on the surface of the polystyrene plate.…”

Section: Discussionmentioning

confidence: 99%

“…As indicated above, such results were observed for ZnO nanoparticles, where lower concentrations accelerated biofilm formation due to the cell response. On the other hand, higher concentrations decreased the biomass formation and the number of biofilm formation centers [ 40 , 41 ]. In the current study, the measured biomass of biofilms did not generally differ from the control samples.…”

Section: Discussionmentioning

confidence: 99%

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The Response of Pseudomonas aeruginosa PAO1 to UV-activated Titanium Dioxide/Silica Nanotubes

Augustyniak

Cendrowski

Grygorcewicz

et al. 2020

IJMS

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Pseudomonas aeruginosa is a bacterium of high clinical and biotechnological importance thanks to its high adaptability to environmental conditions. The increasing incidence of antibiotic-resistant strains has created a need for alternative methods to increase the chance of recovery in infected patients. Various nanomaterials have the potential to be used for this purpose. Therefore, we aimed to study the physiological response of P. aeruginosa PAO1 to titanium dioxide/silica nanotubes. The results suggest that UV light-irradiated nanomaterial triggers strong agglomeration in the studied bacteria that was confirmed by microscopy, spectrophotometry, and flow cytometry. The effect was diminished when the nanomaterial was applied without initial irradiation, with UV light indicating that the creation of reactive oxygen species could play a role in this phenomenon. The nanocomposite also affected biofilm formation ability. Even though the biomass of biofilms was comparable, the viability of cells in biofilms was upregulated in 48-hour biofilms. Furthermore, from six selected genes, the mexA coding efflux pump was upregulated, which could be associated with an interaction with TiO2. The results show that titanium dioxide/silica nanotubes may alter the physiological and metabolic functions of P. aeruginosa PAO1.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

The Response of Pseudomonas aeruginosa PAO1 to UV-activated Titanium Dioxide/Silica Nanotubes

Augustyniak

Cendrowski

Grygorcewicz

et al. 2020

IJMS

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“…[132] To resist the toxicity of TiO 2 NPs, the metabolic activities of periphytic biofilms were elevated in bacterial communities, including those of Alphaproteobacteria, Gammaproteobacteria, Cytophagia, Flavobacteriia, Sphingobacteriia, Synechococcophycideae, and Oscillatoriophycideae. Very recently, a reported study showed that high concentrations of ZnO NPs (>30 mg L −1 ) significantly inhibited biofilm formation of Pseudomonas putida, [133] while low concentrations (0.5-30 mg L −1 ) promoted bacterial growth and biofilm formation. This was achieved by stimulating the expression of quorum sensing, lipopolysaccharide biosynthesis, and antibiotic resistance genes, and corroborating the increased protein and sugar content of the biofilm matrix.…”

Section: Bacteriamentioning

confidence: 99%

The Crucial Role of Environmental Coronas in Determining the Biological Effects of Engineered Nanomaterials

Wang

et al. 2020

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In aquatic environments, a large number of ecological macromolecules (e.g., natural organic matter (NOM), extracellular polymeric substances (EPS), and proteins) can adsorb onto the surface of engineered nanomaterials (ENMs) to form a unique environmental corona. The presence of environmental corona as an eco–nano interface can significantly alter the bioavailability, biocompatibility, and toxicity of pristine ENMs to aquatic organisms. However, as an emerging field, research on the impact of the environmental corona on the fate and behavior of ENMs in aquatic environments is still in its infancy. To promote a deeper understanding of its importance in driving or moderating ENM toxicity, this study systemically recapitulates the literature of representative types of macromolecules that are adsorbed onto ENMs; these constitute the environmental corona, including NOM, EPS, proteins, and surfactants. Next, the ecotoxicological effects of environmental corona‐coated ENMs on representative aquatic organisms at different trophic levels are discussed in comparison to pristine ENMs, based on the reported studies. According to this analysis, molecular mechanisms triggered by pristine and environmental corona‐coated ENMs are compared, including membrane adhesion, membrane damage, cellular internalization, oxidative stress, immunotoxicity, genotoxicity, and reproductive toxicity. Finally, current knowledge gaps and challenges in this field are discussed from the ecotoxicology perspective.

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“…The EPS that forms the aggregate matrix acts as a physical barrier that hinders NP penetration and traps them at the periphery, thus reducing bacteria exposure [20,66], or even modifying the properties of NPs, diminishing their reactivity and their antimicrobial effect [54,66] (Figure 1B). Furthermore, limiting the penetration of NPs may allow bacteria in the interior of the aggregate to sense sub-lethal concentrations and develop an adaptive response that indeed increases NP resistance and enhances biofilm growth [67]. This phenomenon is called hormesis, and it is defined as a process in which exposure to a low dose of a chemical that is deleterious at high doses induces an adaptive beneficial effect on the cell [68].…”

Section: Bacterial Resistance Mechanisms Towards Metallic Nanoparticlesmentioning

confidence: 99%

“…Hormesis has been observed in different bacteria exposed to some antibiotics and metallic NPs. For example, sub-lethal doses of ZnO-NPs and Ag-NPs were found to promote growth of P. putida and P. aeruginosa biofilms, respectively, by inducing the expression of quorum sensing and LPS biosynthesis genes as well as the release of signal molecules by bacteria [67,69]. Similarly, S. oneidensis MR-1 was reported to increase the production of EPS when exposed to Cu-doped TiO2-NPs [70].…”

Section: Bacterial Resistance Mechanisms Towards Metallic Nanoparticlesmentioning

confidence: 99%

Metallic Nanoparticles—Friends or Foes in the Battle against Antibiotic-Resistant Bacteria?

et al. 2021

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The rapid spread of antibiotic resistances among bacteria demands novel strategies for infection control, and metallic nanoparticles appear as promising tools because of their unique size and tunable properties that allow their antibacterial effects to be maximized. Furthermore, their diverse mechanisms of action towards multiple cell components have suggested that bacteria could not easily develop resistance against nanoparticles. However, research published over the last decade has proven that bacteria can indeed evolve stable resistance mechanisms upon continuous exposure to metallic nanoparticles. In this review, we summarize the currently known individual and collective strategies employed by bacteria to cope with metallic nanoparticles. Importantly, we also discuss the adverse side effects that bacterial exposure to nanoparticles may have on antibiotic resistance dissemination and that might constitute a challenge for the implementation of nanoparticles as antibacterial agents. Overall, studies discussed in this review point out that careful management of these very promising antimicrobials is necessary to preserve their efficacy for infection control.

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Towards a better understanding of Pseudomonas putida biofilm formation in the presence of ZnO nanoparticles (NPs): Role of NP concentration

Cited by 61 publications

References 77 publications

The Response of Pseudomonas aeruginosa PAO1 to UV-activated Titanium Dioxide/Silica Nanotubes

The Response of Pseudomonas aeruginosa PAO1 to UV-activated Titanium Dioxide/Silica Nanotubes

The Crucial Role of Environmental Coronas in Determining the Biological Effects of Engineered Nanomaterials

Metallic Nanoparticles—Friends or Foes in the Battle against Antibiotic-Resistant Bacteria?

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