2006
DOI: 10.1002/cphc.200600507
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Towards a Detailed Understanding of Bacterial Metabolism—Spectroscopic Characterization of Staphylococcus Epidermidis

Abstract: Bacteria are a major cause of infection. To fight disease and growing resistance, research interest is focused on understanding bacterial metabolism. For a detailed evaluation of the involved mechanisms, a precise knowledge of the molecular composition of the bacteria is required. In this article, various vibrational spectroscopic techniques are applied to comprehensively characterize, on a molecular level, bacteria of the strain Staphylococcus epidermidis, an opportunistic pathogen which has evolved to become… Show more

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Cited by 196 publications
(177 citation statements)
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“…Figure 3 shows averaged Raman spectra for P. aeruginosa biofilm. It is characterized by vibrational bands typical for nucleic acids, proteins, lipids, carbohydrates and carotenoids (see Table 1) [19, [39][40][41][42]. The prominent Raman bands of biofilm belong to proteins: 1002 cm −1 (phenylalanine), 1243 cm −1 (amide III) and 1658 cm −1 (amide I).…”
Section: Resultsmentioning
confidence: 99%
“…Figure 3 shows averaged Raman spectra for P. aeruginosa biofilm. It is characterized by vibrational bands typical for nucleic acids, proteins, lipids, carbohydrates and carotenoids (see Table 1) [19, [39][40][41][42]. The prominent Raman bands of biofilm belong to proteins: 1002 cm −1 (phenylalanine), 1243 cm −1 (amide III) and 1658 cm −1 (amide I).…”
Section: Resultsmentioning
confidence: 99%
“…For resonance enhancement, the absorbing photon is of sufficient energy to excite a chromophore into an electronic excited state, which results in a markedly increased probability that the scattered photon differs in energy by an amount that corresponds to the energy of a molecular vibration. This can increase the intensity of the Raman effect by up to six orders of magnitude and can, for instance, be used to selectively monitor heme proteins in biological samples using visible excitation into the Soret or Q bands (Wood et al 2005) or selective enhancement of nucleic acids and certain aromatic amino acid residues in proteins by UV excitation (Neugebauer et al 2007). This advantage, however, can often be outweighed by fluorescence and photodamage caused by such irradiation; thus, NIR excitation is also often used in dispersive Raman measurements with biological samples, albeit using shorter wavelength excitation than is used in FT-Raman spectroscopy, in order to enhance the Raman effect.…”
Section: Raman Spectroscopic Microprobesmentioning
confidence: 99%
“…Neugebauer et al measured TERS spectra from Staphylococcus epidermidis [370,371], while Cialla et al measured TERS spectra from tobacco mosaic viruses [372]. Other bacteria-derived samples, such as isolated membrane patches from Halobacterium salinarum have also been measured with TERS, with different patches able to be distinguished [373].…”
Section: Applicationsmentioning
confidence: 99%