purpose: The objective of this article is to examine the effect of transformational leadership on collective efficacy and organizational citizenship behaviour on cross-functional teams’ members. The study also investigates the mediating and moderating effects in the relationship. Design/methodology/approach: Self-structured questionnaire with a 5-point Likert scale was used to collect the data by adopting both online and offline mode. A sample of 414 team members was drawn from working cross-functional teams of different service sectors, that is, transportation, banks, hospitals, information technology, hotels, educational institutions, agricultural irrigation services, insurance, construction and from real estate in the North India region. PLS-SEM was used to test the relationship between variables. PLS-MGA approach was used to test the moderating effect. Findings: The results revealed the positive and significant effect of transformational leadership on collective efficacy and organizational citizenship behaviour (OCB). The finding also supports the mediating effect of collective efficacy on the relationship. The moderating effect of gender also reveals the significant difference between the relationship between transformational leadership and collective efficacy. Research limitations/implications: The study will contribute towards understanding the mechanism of cross-functional teams through which an organization can manage the teamwork effectively. The study acknowledges the small sample limitation with a few service sector industries. Practical implications: Practitioners can develop a positive belief among team members by concentrating on the quality of leadership and the working environment of the team as well. Originality/value: This study probes the transformational leadership and collective efficacy variables as key variables that can make a positive change in the behavioural and performance perspective of team members.