Towards a Mechanism for Poly(I·C) Antiviral Priming in Oysters

Martins, Nelson

doi:10.1128/mbio.00407-20

Cited by 8 publications

(3 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Single AP-MS experiments, therefore, need to be carefully controlled and allow only partial insights into the specificities for individual viral NAs. Overall, we identified a set of conserved interactants, underscoring the ancient origins of antiviral innate immunity 80 , together with a number of species-specific candidates, reflecting differences in the evolutionary trajectories of antiviral immune systems in organisms independently confronted to specific viruses 81 . Our results provide a useful resource for future functional studies and highlight the potential of the approach, which could be used with other species (e.g., other vertebrates, vector mosquitoes, and nematodes) to provide broader phylogenetic perspectives.…”

Section: Discussionmentioning

confidence: 99%

Cross-species analysis of viral nucleic acid interacting proteins identifies TAOKs as innate immune regulators

et al. 2021

View full text Add to dashboard Cite

The cell intrinsic antiviral response of multicellular organisms developed over millions of years and critically relies on the ability to sense and eliminate viral nucleic acids. Here we use an affinity proteomics approach in evolutionary distant species (human, mouse and fly) to identify proteins that are conserved in their ability to associate with diverse viral nucleic acids. This approach shows a core of orthologous proteins targeting viral genetic material and species-specific interactions. Functional characterization of the influence of 181 candidates on replication of 6 distinct viruses in human cells and flies identifies 128 nucleic acid binding proteins with an impact on virus growth. We identify the family of TAO kinases (TAOK1, −2 and −3) as dsRNA-interacting antiviral proteins and show their requirement for type-I interferon induction. Depletion of TAO kinases in mammals or flies leads to an impaired response to virus infection characterized by a reduced induction of interferon stimulated genes in mammals and impaired expression of srg1 and diedel in flies. Overall, our study shows a larger set of proteins able to mediate the interaction between viral genetic material and host factors than anticipated so far, attesting to the ancestral roots of innate immunity and to the lineage-specific pressures exerted by viruses.

show abstract

Section: Discussionmentioning

confidence: 99%

Cross-species analysis of viral nucleic acid interacting proteins identifies TAOKs as innate immune regulators

et al. 2021

View full text Add to dashboard Cite

show abstract

“…The recent discovery of IFN-like induced responses to virus infection in oysters (Lafont et al, 2020;Martins, 2020) and the conservation of the cGAS-STING pathway in the sea anemone Nematostella vectensis (Kranzusch et al, 2015) suggest that ancestral metazoan antiviral immunity involved induced expression of antiviral factors.…”

Section: Inducible Responses To Virus Infection In Fliesmentioning

confidence: 99%

Sensing and signalling viral infection in drosophila

Schneider¹,

Imler²

2021

Developmental & Comparative Immunology

View full text Add to dashboard Cite

The fruitfly Drosophila melanogaster is a valuable model to unravel mechanisms of innate immunity, in particular in the context of viral infections. RNA interference, and more specifically the small interfering RNA pathway, is a major component of antiviral immunity in drosophila. In addition, the contribution of inducible transcriptional responses to the control of viruses in drosophila and other invertebrates is increasingly recognized. In particular, the recent discovery of a STING-IKK -Relish signalling cassette in drosophila has confirmed that NF-B transcription factors play an important role in the control of viral infections, in addition to bacterial and fungal infections. Here, we review recent developments in the field, which begin to shed light on the mechanisms involved in sensing of viral infections and in signalling leading to production of antiviral effectors.

show abstract

“…Poly I:C is a double stranded, synthetic RNA molecule eliciting a transient antiviral state in vitro and in vivo in vertebrates [36] and invertebrates [37] mainly through potent interferon induction. Injection of poly I:C in fish confers resistance to viral infections lasting up to several weeks [38][39][40].…”

Section: Introductionmentioning

confidence: 99%

Effects of eicosapentaneoic acid on innate immune responses in an Atlantic salmon kidney cell line in vitro

Gjøen,

Ruyter,

Østbye

2024

PLoS ONE

View full text Add to dashboard Cite

Studies of the interplay between metabolism and immunity, known as immunometabolism, is steadily transforming immunological research into new understandings of how environmental cues like diet are affecting innate and adaptive immune responses. The aim of this study was to explore antiviral transcriptomic responses under various levels of polyunsaturated fatty acid. Atlantic salmon kidney cells (ASK cell line) were incubated for one week in different levels of the unsaturated n-3 eicosapentaneoic acid (EPA) resulting in cellular levels ranging from 2–20% of total fatty acid. These cells were then stimulated with the viral mimic and interferon inducer poly I:C (30 ug/ml) for 24 hours before total RNA was isolated and sequenced for transcriptomic analyses. Up to 200 uM EPA had no detrimental effects on cell viability and induced very few transcriptional changes in these cells. However, in combination with poly I:C, our results shows that the level of EPA in the cellular membranes exert profound dose dependent effects of the transcriptional profiles induced by this treatment. Metabolic pathways like autophagy, apelin and VEGF signaling were attenuated by EPA whereas transcripts related to fatty acid metabolism, ferroptosis and the PPAR signaling pathways were upregulated. These results suggests that innate antiviral responses are heavily influenced by the fatty acid profile of salmonid cells and constitute another example of the strong linkage between general metabolic pathways and inflammatory responses.

show abstract

Towards a Mechanism for Poly(I·C) Antiviral Priming in Oysters

Cited by 8 publications

References 11 publications

Cross-species analysis of viral nucleic acid interacting proteins identifies TAOKs as innate immune regulators

Cross-species analysis of viral nucleic acid interacting proteins identifies TAOKs as innate immune regulators

Sensing and signalling viral infection in drosophila

Effects of eicosapentaneoic acid on innate immune responses in an Atlantic salmon kidney cell line in vitro

Contact Info

Product

Resources

About