Stationary phases containing pyridine, phenol, aniline and morpholine groups were synthesized using copper (I)-catalyzed azide-alkyne cycloaddition click reactions. The backbone of the stationary phases was aminopropyl silica. The stationary phases were evaluated in packed-column supercritical fluid chromatography (pSFC) with acidic and basic solutes without addition of additives. The analysis of metoclopromide and its impurities by SFC-time-of-flight mass spectrometry (SFC-TOFMS) on the phenol phase is presented. In the click reaction, the 1,2,3-triazole ring is formed and to assess its influence on the polarity/selectivity, the click phases were compared to a commercial available 1,2,4-triazole hydrophilic interaction liquid chromatography (HILIC) phase. The phases were also compared to two extensively used stationary phases in SFC namely 2-ethyl pyridine and bare silica. To allow comparison with other phases used in SFC, linear solvation energy relationships (LSER) of the click phases were determined. Synthesis of stationary phases containing pyridine, phenol, aniline and morpholine via click chemistry and their characterization and evaluation in supercritical fluid chromatography Dunkle M et al. Synthesis of stationary phases containing pyridine, phenol, aniline and morpholine 86 Scientia Chromatographica 2014; 6(2):85-103 Synthesis of stationary phases containing pyridine, phenol, aniline and morpholine Dunkle M et al. Scientia Chromatographica 2014; 6(2):85-103