Towards a reference map of Eimeria tenella sporozoite proteins by two-dimensional electrophoresis and mass spectrometry

Model structure of the immunodominant surface antigen of Eimeria tenella identified as a target for sporozoite-neutralizing monoclonal antibody

“…Characterization of the corresponding recombinant counterparts confirmed that EtSAG1 was the only target antigen, whereas the 19-kDa sporozoite antigen of E. tenella was attributed to be as a co-eluting protein of similar molecular weight and isoelectric point. EtSAG1 has already been found previously to be a molecular target for a number of sporozoite-neutralizing antibodies using similar 2D electrophoresis approach (Brothers et al 1988;de Venevelles et al 2004). However, identification of the corresponding antigen was performed in our study using much smaller amounts of the parasite material, i.e., only 35 μg of oocyst extract vs. 0.1-1.0 mg in earlier reports (de Venevelles et al 2004;Sutton et al 1989).…”

“…EtSAG1 has already been found previously to be a molecular target for a number of sporozoite-neutralizing antibodies using similar 2D electrophoresis approach (Brothers et al 1988;de Venevelles et al 2004). However, identification of the corresponding antigen was performed in our study using much smaller amounts of the parasite material, i.e., only 35 μg of oocyst extract vs. 0.1-1.0 mg in earlier reports (de Venevelles et al 2004;Sutton et al 1989). To confirm the antigen specificity, we expressed EtSAG1 as a soluble recombinant protein correctly folded in bacterial periplasm, which could be purified under nondenaturing conditions in reasonable yields.…”

Excretory/secretory proteome of 14-day schistosomula, Schistosoma japonicum

“…Carbohydrate metabolism is a major source of energy that supports parasite survival in an anaerobic environment and is also involved in antioxidative processes [41]. Enzymes associated with carbohydrate metabolism have traditionally been considered to be cytosolic; however, recent studies have shown that they can be secreted and detected on external surfaces of different organisms, such as nematodes [21], trematodes [42], protozoa [43], cestodes [44], and even mammals [45]. Some of these proteins were identified among the schistosomula ES products of S. japonicum.…”

Molecular cloning, sequencing, and expression of Eimeria tenella HSP70 partial gene

“…These cytokines are associated with a strong Th1 response, which is the main immune response against intracellular parasites such as those of the Eimeria genus . The use of recombinant proteins for assessing the ability to stimulate protective immunity against avian coccidiosis has been studied previously de Venevelles et al, 2004;Ding et al, 2005;Liu et al, 2009;Song et al, 2013), with the aim of producing an alternative to the anticoccidial agents used in poultry feed.…”

“…Therefore, the sequence was deposited in GenBank (KT364872) with the appropriate annotations for introns and exons. Other researchers have evaluated the HSP70 proteins of Eimeria spp in terms of sequencing, expression, and antigenic/immunological characterization (Laurent et al, 1994;Bumstead et al, 1995;Dunn et al, 1995;Brown et al, 2000;del Cacho et al, 2001;de Venevelles et al, 2004;del Cacho et al, 2005;Del Cacho et al, 2008;Liu et al, 2009;Zhang et al, 2012). Studies on HSP70 from other species of microorganisms also suggest it has immunogenic potential, as observed by Li et al (2011) when evaluating the HSP70 of Mycoplasma ovipneumoniae in sheep.…”