Towards a systems-based understanding of plant desiccation tolerance

Moore, John P.; Le, Ngoc Tuan; Brandt, Wolf F.; Driouich, Azeddine; Farrant, Jill M.

doi:10.1016/j.tplants.2008.11.007

Cited by 166 publications

(130 citation statements)

References 74 publications

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“…Interestingly, exactly the same band was shown to be shifted during desiccation-induced conformational changes of a recombinant LEA (Late embryogenesis abundant) protein (Tolleter et al 2007). Structurally considered to be random coil, LEA proteins have been shown to form stabilized structures in the vicinity of membranes and they can act as antiaggregants, protecting the proteomes against water loss induced aggregation (Moore et al 2009). LEA are extremely hydrophilic proteins produced at high levels in seeds and pollen grains during the latter stages of maturation and desiccation that are ubiquitously produced by plants, algae and cyanobacteria in response to water stress (Rascio & La Rocca 2005), where LEA proteins can reach as much as 40% of the dry weight in some resurrection plants (Gechev et al 2012).…”

Section: Discussionmentioning

confidence: 92%

“…Poikilochlorophyllous resurrection plants degrade their chlorophyll and resynthesize it after rehydration. Homoiochlorophyllous are subjected to a greater risk of photo-oxidative and metabolic damage occurring because active photosystems can be uncoupled from metabolic dissipation mechanisms, resulting in oxidative damage (Moore et al 2009), which is minimized by additional morphological and biochemical mechanisms such as leaf folding to reduce absorbed radiation or/and accumulation of anthocyanins and other phenolic compounds to protect against solar radiation (Farrant & Moore 2011).…”

Section: Introductionmentioning

confidence: 99%

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Reversible in vivo cellular changes occur during desiccation and recovery: Desiccation tolerance of the resurrection filmy fern Hymenophyllum dentatum Cav.

Bravo

Parra²,

Castillo³

et al. 2016

Gayana Bot.

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Reversible in vivo cellular changes occur during desiccation and recovery: Desiccation tolerance of the resurrection filmy fern Hymenophyllum dentatum Cav.Cambios celulares reversibles observados in vivo durante la desecación y recuperación: Tolerancia a la desecación del helecho película de resurrección Hymenophyllum dentatum Cav. SORAYA ABSTRACTThe present work explores in vivo physiological, morphological and chemical features during full hydration, desiccation and rehydration of the filmy fern Hymenophyllum dentatum with two main objectives: 1) to get further insight about the mechanisms underlying its desiccation tolerance, and 2) to understand how this plant manages mechanical stress induced by water loss and recovery. With these purposes, physiological (relative water content and F v /F m chlorophyll fluorescence parameter), morphological (Confocal Laser Scanning Microscopy and 3D reconstruction) and chemical (FT-IR microspectroscopy) data were obtained and compared between fully hydrated, desiccated and rehydrated tissues of H. dentatum. Remarkable changes in cell architecture and chemical composition were observed in vivo in desiccated leaves. Cells were smaller, showed a collapsed general appearance, and were delimitated by apparently folded cell walls. Marked changes in chloroplasts location and decrease in the number of active chloroplasts were also evidenced. Chemical experiments showed that changes in the secondary structure of proteins and in the polysaccharide composition of the cell wall occur in desiccated cells. All changes were rapidly reversed upon rehydration. This study shows that H. dentatum presents an extreme case of desiccation tolerance, able to withdraw severe, rapid and consecutive dehydration/rehydration induced stress by the function of constitutive systems of protection and reparation, in which cell wall folding plays a relevant role as a protective system against mechanical and oxidative stress. Besides, H. dentatum is proposed as an excellent plant model for the study of dissection tolerance such as one cell layer fern, auto-fluorescence of cellular compartments, and simple long term storage under laboratory conditions, among others. KEYWORDS:Hymenophyllum, desiccation tolerance, confocal 3D microscopy, FT-IR microspectroscopy, rapid dehydration /rehydration. RESUMENEl presente trabajo muestra una exploración in vivo de los rasgos fisiológicos, morfológicos y químicos que caracterizan a los estados de hidratación completa, desecación y rehidratación del helecho película Hymenophyllum dentatum, que persigue 2 objetivos principales: 1) Adquirir conocimientos sobre los mecanismos que subyacen a la tolerancia a la desecación, y 2) entender cómo estas plantas manejan el estrés mecánico inducido por la pérdida y recuperación de agua. Con estos propósitos se obtuvo datos fisiológicos (contenido relativo de agua y parámetro de fluorescencia de clorofila Fv/Fm), ISSN 0016-5301 Gayana Bot. 73(2): [402][403][404][405][406][407][408][409][410][411][412][413] 2016 403In vivo cellular ch...

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Section: Discussionmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Reversible in vivo cellular changes occur during desiccation and recovery: Desiccation tolerance of the resurrection filmy fern Hymenophyllum dentatum Cav.

Bravo

Parra²,

Castillo³

et al. 2016

Gayana Bot.

View full text Add to dashboard Cite

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“…It is also necessary to understand how the system might be affected by the interplay of external factors (e.g., water availability) and internal factors (developmental triggers). Therefore, analyses of the system structures (e.g., gene interactions) and dynamics (e.g., metabolic fluxes) become important (Kitano 2002;Moore et al 2009). Correlation of next-generation sequencing, genomescale molecular analysis, modeling of physiological and molecular data to the physiology of the plant leads to new data about adaptability and improved traits which can ultimately be incorporated in crop plants to improve productivity under stress (weckwerth 2011).…”

Section: Introductionmentioning

confidence: 99%

A systems biology perspective on the role of WRKY transcription factors in drought responses in plants

Tripathi

Rabara

Rushton

2013

Planta

203

130

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“…In lichens, both desiccation and rehydration occur rapidly due to the absence of a waxy cuticullar barrier to water exchanges, making both processes potentially more harmful than in plants. For example, some desiccation-tolerant vascular plants ("resurrection plants") degrade their chlorophyll during dehydration and resynthesize it after rehydration [31], thus preventing unbalanced ROS production. However, lichen photobionts do not significantly alter their content of photosynthetic pigments during their rapid and cyclic changes in water content.…”

Section: Introductionmentioning

confidence: 99%

Lichen Rehydration in Heavy Metal-Polluted Environments: Pb Modulates the Oxidative Response of Both Ramalina farinacea Thalli and Its Isolated Microalgae

et al. 2014

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Lichens are adapted to desiccation/rehydration and accumulate heavy metals, which induce ROS especially from the photobiont photosynthetic pigments. Although their mechanisms of abiotic stress tolerance are still to be unravelled, they seem related to symbionts' reciprocal upregulation of antioxidant systems. With the aim to study the effect of Pb on oxidative status during rehydration, the kinetics of intracellular ROS, lipid peroxidation and chlorophyll autofluorescence of whole Ramalina farinacea thalli and its isolated microalgae (Trebouxia TR1 and T. TR9) was recorded. A genetic characterization of the microalgae present in the thalli used was also carried out in order to assess possible correlations among the relative abundance of each phycobiont, their individual physiological responses and that of the entire thallus. Unexpectedly, Pb decreased ROS and lipid peroxidation in thalli and its phycobionts, associated with a lower chlorophyll autofluorescence. Each phycobiont showed a particular pattern, but the oxidative response of the thallus paralleled the TR1's, agreeing with the genetic identification of this strain as the predominant phycobiont. We conclude that: (1) the lichen oxidative behaviour seems to be modulated by the predominant phycobiont and (2) Pb evokes in R. farinacea and its phycobionts strong mechanisms to neutralize its own oxidant effects along with those of rehydration.

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Towards a systems-based understanding of plant desiccation tolerance

Cited by 166 publications

References 74 publications

Reversible in vivo cellular changes occur during desiccation and recovery: Desiccation tolerance of the resurrection filmy fern Hymenophyllum dentatum Cav.

Reversible in vivo cellular changes occur during desiccation and recovery: Desiccation tolerance of the resurrection filmy fern Hymenophyllum dentatum Cav.

A systems biology perspective on the role of WRKY transcription factors in drought responses in plants

Lichen Rehydration in Heavy Metal-Polluted Environments: Pb Modulates the Oxidative Response of Both Ramalina farinacea Thalli and Its Isolated Microalgae

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