Towards a Tissue-Engineered Ligament: Design and Preliminary Evaluation of a Dedicated Multi-Chamber  Tension-Torsion Bioreactor

Laurent, Cédric; Vaquette, Cédryck; Martin, Céline; Guédon, Emmanuel; Wu, Xiude; Delconte, Alain; Dumas, Dominique; Hupont, Sébastien; Isla, Natalia de; Rahouadj, Rachid; Wang, Xiong

doi:10.3390/pr2010167

Cited by 19 publications

(24 citation statements)

References 41 publications

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“…PLCL is an elastomeric synthetic copolymer combining biocompatible, degradable PLLA and PCL, resulting in desirable mechanical properties. It is of great interest in tissue engineering, because of its biocompatibility, biodegradability, and easy processing. Vuornos et al compared a foamed PLCL(70/30) structure with a braided poly( l / d )lactide (PLA, 96 L/4D) scaffold for tendon tissue engineering, and PLCL was judged to be excessively elastic to form a braided structure in their study.…”

Section: Discussionmentioning

confidence: 99%

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Mesenchymal stem cell interacted with PLCL braided scaffold coated with poly‐l‐lysine/hyaluronic acid for ligament tissue engineering

Liu

Laurent

et al. 2018

J Biomedical Materials Res

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The challenge of finding an adapted scaffold for ligament tissue engineering remains unsolved after years of researches. A technology to fabricate a multilayer braided scaffold with flexible and elastic poly (l-lactide-co-caprolactone) (PLCL 85/15) has been recently pioneered by our team. In this study, polyelectrolyte multilayer films (PEM) with poly-l-lysine (PLL)/ hyaluronic acid (HA) were deposited on this scaffold. After PEM modification, polygonal (PLL) and particle-like (HA) structures were present on the braided scaffold with no significant variation of fibers Young's modulus. Wharton's jelly mesenchymal stem cells (WJ-MSC) and bone marrow mesenchymal stem cells (BM-MSC) showed good metabolic activity on scaffolds. They presented a spindled shape along the fiber longitudinal direction, and crossed the fibers to form cell bridges. Collagen type I, collagen type III, and tenascin-C secreted by MSCs were detected on day 14. Moreover, one-layer modified scaffold presented increased chemotaxis. As a conclusion, our results indicate that this braided PLCL scaffold with one-layer PEM modification shows inspiring potential with satisfying mechanical properties and biocompatibility. It opens new perspectives to incorporate growth factors within PEM-modified braided PLCL scaffold for ligament tissue engineering and to recruit endogenous cells after implantation. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 3042-3052, 2018.

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Section: Discussionmentioning

confidence: 99%

“…PLCL (85/15) braided scaffold was fabricated according to previously described methods . Briefly, it consists of a multilayer braided structure, each layer being made up of 16 fibers of poly(lactide‐co‐ε‐caprolactone) (PLCL, Purac Biomaterials) with a lactic acid/ε‐caprolactone proportion of 85/15.…”

Section: Methodsmentioning

confidence: 99%

Mesenchymal stem cell interacted with PLCL braided scaffold coated with poly‐l‐lysine/hyaluronic acid for ligament tissue engineering

Liu

Laurent

et al. 2018

J Biomedical Materials Res

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“…Another important aspect is to understand if the proposed experimental conditions in bioreactors result in highly reproducible outcomes, so that suitable bone engineering protocols can be developed for translational applications. The use of miniature multi-chamber bioreactor systems has been proposed to address the problem of the large variability associated with the use of biological material, resulting for example from genetic and developmental differences of the cell source used to engineering tissues [85,86]. Bioreactor systems support dynamic maturation of tissue substitutes by providing physical stimulation to the cells in the three-dimensional context within biomaterial scaffolds.…”

Section: Future Directions For Clinical Translationmentioning

confidence: 99%

Bioreactor Systems for Human Bone Tissue Engineering

Sladkova

Peppo

2014

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Critical size skeletal defects resulting from trauma and pathological disorders still remain a major clinical problem worldwide. Bone engineering aims at generating unlimited amounts of viable tissue substitutes by interfacing osteocompetent cells of different origin and developmental stage with compliant biomaterial scaffolds, and culture the cell/scaffold constructs under proper culture conditions in bioreactor systems. Bioreactors help supporting efficient nutrition of cultured cells and allow the controlled provision of biochemical and biophysical stimuli required for functional regeneration and production of clinically relevant bone grafts. In this review, the authors report the advances in the development of bone tissue substitutes using human cells and bioreactor systems. Principal types of bioreactors are reviewed, including rotating wall vessels, spinner flasks, direct and indirect flow perfusion bioreactors, as well as compression systems. Specifically, the review deals with: (i) key elements of bioreactor design; (ii) range of values of stress imparted to cells and physiological relevance; (iii) maximal volume of engineered bone substitutes cultured in different bioreactors; and (iv) experimental outcomes and perspectives for future clinical translation.

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“…That dynamic culture, when compared with a static culture, presented a high cellular adhesion and proliferation in 28 days. 25 …”

Section: Cell Seeding and Delivery Of Growth Factorsmentioning

confidence: 99%

Current Approaches and Future Trends to Promote Tendon Repair

et al. 2015

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Tendons are composed by extracellular collagen fibres arranged in regular arrays and are responsible to transmit tensile forces from a muscle to a bone. Due to their poor healing ability, in some cases tendons injuries are debilitating impairments that affect life quality among adult population worldwide. In the last years, attending to the social and economic concern associated to the high prevalence of tendons injuries and the limited success of the available current treatments, several scaffolds have been developed. Some of these scaffolds are intended to be used as graft-augmentation devices and others to fully replace a damaged tendon. The synthetic ones present superior mechanical characteristics compared to biological scaffolds. However, attending to the specific tendons physiology, even the synthetic scaffolds still don´t present the ideal mechanical properties to accomplish a complete and long-term functional tissue repair. Therefore, to enhance tendogenesis when using a tendon engineering approach, several methodologies have been developed to associate with scaffolds, including surface modification and cell seeding.

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Towards a Tissue-Engineered Ligament: Design and Preliminary Evaluation of a Dedicated Multi-Chamber Tension-Torsion Bioreactor

Cited by 19 publications

References 41 publications

Mesenchymal stem cell interacted with PLCL braided scaffold coated with poly‐l‐lysine/hyaluronic acid for ligament tissue engineering

Mesenchymal stem cell interacted with PLCL braided scaffold coated with poly‐l‐lysine/hyaluronic acid for ligament tissue engineering

Bioreactor Systems for Human Bone Tissue Engineering

Current Approaches and Future Trends to Promote Tendon Repair

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