Towards a traceable enhancement factor in surface-enhanced Raman spectroscopy

Cara, Eleonora; Mandrile, Luisa; Sacco, Alessio; Giovannozzi, Andrea Mario; Rossi, Andrea Mario; Celegato, Federica; Leo, Natascia De; Hönicke, Philipp; Kayser, Yves; Beckhoff, Burkhard; Marchi, Davide; Zoccante, Alberto; Cossi, Maurizio; Laus, Michele; Boarino, Luca; Lupi, Federico Ferrarese

doi:10.1039/d0tc04364h

Cited by 35 publications

(21 citation statements)

References 43 publications

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“…It is also indicated as a suitable matrix for the analysis of small molecular compounds [ 40 ]. The other described incorporation of this highly valuable scaffold is as a reporter molecule [ 41 ], reporters for thiol interactions at the nanoparticle surface [ 42 ], fluorescent probe [ 43 , 44 , 45 ], sugar acceptor [ 46 ], Raman reporter [ 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 ], an excellent substrate for fluorescence spectroscopy [ 57 ], photodimerizable and healable reactant [ 58 ], fluorescent dye [ 59 , 60 ], and probe molecule on gold-coated silicon nanowires [ 61 ].…”

Section: 7-mercapto-coumarinmentioning

confidence: 99%

Insight on Mercapto-Coumarins: Synthesis and Reactivity

El‐Sawy

Abdelwahab²,

Kirsch

2022

Molecules

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Mercapto (or sulfanyl)-coumarins are heterocycles of great interest in the development of valuable active structures in material and biological domains. They represent a highly exploitable class of compounds that open many possibilities for further chemical transformations. The present review aims to draw focus toward the synthetic applicability of various forms of mercapto-coumarins and their representations in pharmaceuticals and industries. This work covers the literature issued from 1970 to 2021.

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Section: 7-mercapto-coumarinmentioning

confidence: 99%

Insight on Mercapto-Coumarins: Synthesis and Reactivity

El‐Sawy

Abdelwahab²,

Kirsch

2022

Molecules

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“…Maps of single bacterial cells were collected in the range 3300-650 cm −1 with a spectral resolution of 5 cm −1 using a 100× objective with a numerical aperture of 0.9 (Figure 1). Before every measurement, calibration of the instrument was achieved with a neon lamp and white LED lamp [18] and verified with monocrystalline silicon and a polystyrene standard. The frequency uncertainty was determined by the grating resolution of 5 cm −1 ; the intensity uncertainty was demonstrated to be lower than 5% using the polystyrene standard.…”

Section: Bacterial Characterization By Raman Imagingmentioning

confidence: 99%

Hyperspectral Chemical Imaging of Single Bacterial Cell Structure by Raman Spectroscopy and Machine Learning

et al. 2021

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In this work, biomolecules, such as membrane proteins, lipids, and DNA, were identified and their spatial distribution was mapped within a single Escherichia coli cell by Raman hyperspectral imaging. Raman spectroscopy allows direct, nondestructive, rapid, and cost-effective analysis of biological samples, minimizing the sample preparation and without the need of chemical label or immunological staining. Firstly, a comparison between an air-dried and a freeze-dried cell was made, and the principal vibrational modes associated to the membrane and nucleic acids were identified by the bacterium’s Raman chemical fingerprint. Then, analyzing the Raman hyperspectral images by multivariate statistical analysis, the bacterium biological status was investigated at a subcellular level. Principal components analysis (PCA) was applied for dimensionality reduction of the spectral data, then spectral unmixing was performed by multivariate curve resolution–alternating least squares (MCR-ALS). Thanks to multivariate data analysis, the DNA segregation and the Z-ring formation of a replicating bacterial cell were detected at a sub-micrometer level, opening the way to real-time molecular analysis that could be easily applied on in vivo or ex vivo biological samples, avoiding long preparation and analysis process.

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“…The knowledge of accurate fluorescence yields in the X-ray regime is of great importance in many areas of physics and technology, such as fundamental physics [1], biological studies [2,3], biomedical [4,5], environmental sciences [6], spectroscopy [7,8], plasma physics [9], nanoelectronics and microelectronics [10,11], and astrophysics [12].…”

Section: Introductionmentioning

confidence: 99%

Experimental and theoretical approaches for determining the K-shell fluorescence yield of carbon

Hönicke,

Unterumsberger,

Guerra

et al. 2021

Preprint

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The knowledge of atomic fundamental parameters, such as the fluorescence yields with low uncertainties, is of decisive importance in elemental quantification involving X-ray fluorescence analysis techniques. However, especially for the low-Z elements, the available literature data are either of poor quality, of unknown or very large uncertainty, or both. For this reason, the K-shell fluorescence yield of carbon was determined in the PTB laboratory at the synchrotron radiation facility BESSY II. In addition, theoretical calculations of the same parameter were performed using the multiconfiguration Dirac-Fock method, including relativistic and quantum electrodynamics (QED) corrections. Both values obtained in this work are compared to the corresponding available literature data.

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Towards a traceable enhancement factor in surface-enhanced Raman spectroscopy

Abstract: Determination of the SERS enhancement factor through the challenging measurement of the molecular surface density by reference-free X-ray fluorescence.

Cited by 35 publications

References 43 publications

Insight on Mercapto-Coumarins: Synthesis and Reactivity

Insight on Mercapto-Coumarins: Synthesis and Reactivity

Hyperspectral Chemical Imaging of Single Bacterial Cell Structure by Raman Spectroscopy and Machine Learning

Experimental and theoretical approaches for determining the K-shell fluorescence yield of carbon

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