Towards an oral influenza vaccine: Comparison between intragastric and intracolonic delivery of influenza subunit vaccine in a murine model

Amorij, J-P; Westra, Tjalke A.; Hinrichs, Wouter L. J.; Huckriede, Anke; Frijlink, Henderik W.

doi:10.1016/j.vaccine.2007.10.045

Cited by 25 publications

(12 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Montanide was only delivered by systemic routes. For col. immunization, mice were kept without food overnight and the vaccinations were performed using a 4.5 Fg dosing catheter (Harvard Apparatus, Holliston, MA) (44). OVA was solubilized in PBS, mixed with Z3-14 or APols like MOMP and used as a negative control.…”

Section: Methodsmentioning

confidence: 99%

Increased Immunoaccessibility of MOMP Epitopes in a Vaccine Formulated with Amphipols May Account for the Very Robust Protection Elicited against a Vaginal Challenge with Chlamydia muridarum

Tifrea

Pal

Popot

et al. 2014

The Journal of Immunology

View full text Add to dashboard Cite

There is a need to implement a vaccine to protect against Chlamydia trachomatis infections. To test a new vaccine mice were immunized with the C. muridarum native major outer membrane protein (nMOMP) solubilized with either amphipol A8-35 or the detergent Z3-14. Ovalbumin was used as a negative control and mice inoculated intranasally with C. muridarum as positive controls. Animals vaccinated with nMOMP mounted strong Chlamydia-specific humoral and cell mediated immune responses. Mice vaccinated with nMOMP/A8-35 had a higher ratio of antibodies to denatured EB over live EB, recognized more synthetic MOMP peptides and had higher neutralizing titers than sera from mice immunized with nMOMP/Z3-14. T-cell lymphoproliferative responses and levels of IFN-γ were also higher in mice vaccinated with nMOMP/A8-35 than with nMOMP/Z3-14. Following immunization animals were challenged intravaginally with C. muridarum. Based on the number of mice with positive vaginal cultures, length of vaginal shedding, total number of positive vaginal cultures and number of Chlamydia inclusion forming units recovered, nMOMP/A8-35 elicited a more robust protection than nMOMP/Z3-14. By depleting T-cells with antibodies we determined that CD4+ and not CD8+ T-cells, mediated the protection elicited by nMOMP/A8-35. Mice were subsequently mated and based on the number of pregnant mice and number of embryos, animals vaccinated with nMOMP/A8-35 or nMOMP/Z3-14 had fertility rates equivalent to the positive control group immunized with live EB and the fertility controls. In conclusion, increased accessibility of epitopes in the nMOMP/A8-35 preparation may account for the very robust protection against infection and disease elicited by this vaccine.

show abstract

Section: Methodsmentioning

confidence: 99%

Increased Immunoaccessibility of MOMP Epitopes in a Vaccine Formulated with Amphipols May Account for the Very Robust Protection Elicited against a Vaginal Challenge with Chlamydia muridarum

Tifrea

Pal

Popot

et al. 2014

The Journal of Immunology

View full text Add to dashboard Cite

show abstract

“…Indeed when considering recombinant influenza antigen, Amorij, et al (2007) have shown that the intragastric delivery of recombinant H3N2 antigen alone induced low HI titres, which were significantly lower than the antigen delivered IM (Amorij et al, 2007). This can be attributed to rapid degradation of the antigen in the GIT and/or their poor uptake by appropriate immune cells (Devriendt et al, 2012).…”

Section: Oral Vaccination Studiesmentioning

confidence: 99%

Characterization and optimization of bilosomes for oral vaccine delivery

Wilkhu

McNeil

Anderson

et al. 2013

Journal of Drug Targeting

View full text Add to dashboard Cite

Oral vaccines offer significant benefits due to the ease of administration, better patient compliance and non-invasive, needle-free administration. However this route is marred by the harsh gastro intestinal environment which is detrimental to many vaccine formats. To address this, a range of delivery systems have been considered including bilosomes; these are bilayer vesicles constructed from non-ionic surfactants combined with the inclusion of bile salts which can stabilise the vesicles in the gastro intestinal tract by preventing membrane destabilisation. The aim of this study was to investigate the effect of formulation parameters on bilosome carriers using Design of Experiments to select an appropriate formulation to assess in vivo. Bilosomes were constructed from monopalmitoyl glycerol, cholesterol, dicetyl phosphate and sodium deoxycholate at different blends ratios. The optimised bilosome formulation was identified and the potential of this formulation as an oral vaccine delivery system were assessed in biodistribution and vaccine efficacy studies. Results showed that the larger bilosomes vesicles (~6 µm versus 2 µm in diameter) increased uptake within the Peyer's patches and were able to reduce median temperature differential change and promote a reduction in viral cell load in an influenza challenge study.3

show abstract

“…Systemic immune responses are needed to meet regulatory criteria for vaccine immunogenicity. In oral vaccine studies using animal models, oral vaccination was shown to induce both systemic and mucosal immune responses as well as protection [99–105]. The efficacy of non-live mucosal vaccines via oral delivery can be significantly increased by incorporating into enteric-coated gelatin capsules and copolymer microparticles, liposomes, or proteosomes [106], which are attractive targets for M cells.…”

Section: Mucosal Immunizationmentioning

confidence: 99%