Towards engineering integrated cardiac organoids: beating recorded

Zhang, Yu Shrike; Yu, Cunjiang

doi:10.21037/jtd.2016.12.37

Cited by 6 publications

(4 citation statements)

References 30 publications

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“…With the built-in fluid exchange capability of this microfluidic chip, multiple tumor spheroids could be cleared within 1 day by removing the scattering lipid molecules post crosslinking, allowing for high-throughput tissue imaging of intact organoids, analyses of tumor biomarkers and microstructures, and, potentially, investigations of nanomedicine-cancer organoid interactions. Alternatively, sensing units can be built into the organoids for conformal and real-time behavioral monitoring [73]. …”

Section: Discussionmentioning

confidence: 99%

Cancer-on-a-chip systems at the frontier of nanomedicine

Zhang

2017

Drug Discovery Today

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113

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Nanomedicine provides a unique opportunity for promoting drug efficacy through enhanced delivery mechanisms. However, its translation into the clinics has been relatively slow compared with the large amount of research occurring in laboratory settings. Given the limitations of conventional cell culture models and preclinical animal models, we discuss the potential utility of recently developed cancer-on-a-chip platforms, which maximally replicate the pathophysiology of the human tumor microenvironments, as alternatives for effective evaluation of nanomedicine. We begin with a brief discussion of nanomedicine, then chart the history of organ-on-a-chip platform development and their recent evolution as tools for modeling different cancers for assessing nanomedicine efficacy, concluding with future perspectives for the field.

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Section: Discussionmentioning

confidence: 99%

Cancer-on-a-chip systems at the frontier of nanomedicine

Zhang

2017

Drug Discovery Today

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113

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“…Limited study of purpose-bred dogs could also enable accurate targeting, and results which are directly translatable to pet dogs. Microphysiological approaches, such as cardiac organoids and 'heart-on-a-chip' devices (Zhang & Yu, 2016) are capable of recapitulating some of the physiology of the beating heart, and in the future, could become an alternative approach to the study of RIHD, including arrhythmogenesis.…”

Section: Ta B L Ementioning

confidence: 99%

Feasibility study evaluating arrhythmogenesis and cardiac damage after heart‐base irradiation in mice: A brief communication

Elliott

Linder

Nolan

2020

Veterinary Medicine & Sci

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Radiation‐induced heart disease (RIHD) is a potential cause of morbidity and mortality in dogs undergoing thoracic irradiation. Arrhythmias and sudden death have been documented in dogs undergoing stereotactic body radiation therapy for heart base tumours. A study was proposed to interrogate the effect of different stereotactic‐like radiation prescriptions on RIHD development, including arrhythmogenesis and classical histological endpoints in a mouse model. A pilot study was performed initially. The heart base of CD1 (n = 3) and C57Bl/6J (n = 3) female mice were irradiated (12 Gy × 3, daily) with a clinical linear accelerator. No significant adverse effects were noted and each mouse survived the entire subsequent 3‐month observation period. At various time points, no arrhythmias were identified on ECG analysis. Cardiac histology (haematoxylin and eosin, and picrosirius red staining) was performed at 3 months. In a single CD1 mouse and two C57BI/6J mice, multifocal, minimal, peri‐vascular lymphoplasmacytic inflammation was noted within the irradiated proximal heart. In one mouse of each strain, a small, single focus of fibrinoid vascular necrosis was observed. Overall, there was no significant myocardial necrosis, atrophy or inflammation. Picrosirius red staining revealed no evidence of fibrosis in any mouse. Dosimetric verification indicated that the irradiation was successful and delivered as planned, with an average predicted‐to‐measured dose‐difference within 5%. While this study did not demonstrate significant arrhythmogenesis, certain modifications of the experimental mouse irradiation procedures are discussed which may enable more translationally relevant modelling of the canine cardiac response to SBRT‐like irradiation.

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“…Despite rigorous advances in the culturing of physiologically optimized organoids in the relevant field, there is limited applicability due to inadequate tools for practical clinical translation. The integration of engineering innovation into complex organoid systems for more in-depth control and recording of mechanophysiological parameters has proved to be a problematic task. − The high culturing variability of organoids can be managed by engineering a potential design for physical cues during the self-organization processes. − Modern organs-on-a-chip devices have established systemic characterization and controlled synthesis of target tissue elements, which has improved the replicability of functional readouts significantly and extended their lifespans. − However, these devices restrict the natural self-organizing process of growing cell aggregates. The expression of the physiological properties is hampered when using such devices with those constraining conditions.…”

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confidence: 99%

Multimodal Characterization of Cardiac Organoids Using Integrations of Pressure-Sensitive Transistor Arrays with Three-Dimensional Liquid Metal Electrodes

et al. 2022

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Herein, we present an unconventional method for multimodal characterization of three-dimensional cardiac organoids. This method can monitor and control the mechanophysiological parameters of organoids within a single device. In this method, local pressure distributions of human-induced pluripotent stem-cell-derived cardiac organoids are visualized spatiotemporally by an active-matrix array of pressure-sensitive transistors. This array is integrated with three-dimensional electrodes formed by the high-resolution printing of liquid metal. These liquid-metal electrodes are inserted inside an organoid to form the intraorganoid interface for simultaneous electrophysiological recording and stimulation. The low mechanical modulus and low impedance of the liquid-metal electrodes are compatible with organoids’ soft biological tissue, which enables stable electric pacing at low thresholds. In contrast to conventional electrophysiological methods, this measurement of a cardiac organoid’s beating pressures enabled simultaneous treatment of electrical therapeutics using a single device without any interference between the pressure signals and electrical pulses from pacing electrodes, even in wet organoid conditions.

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Towards engineering integrated cardiac organoids: beating recorded

Cited by 6 publications

References 30 publications

Cancer-on-a-chip systems at the frontier of nanomedicine

Cancer-on-a-chip systems at the frontier of nanomedicine

Feasibility study evaluating arrhythmogenesis and cardiac damage after heart‐base irradiation in mice: A brief communication

Multimodal Characterization of Cardiac Organoids Using Integrations of Pressure-Sensitive Transistor Arrays with Three-Dimensional Liquid Metal Electrodes

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