Towards on-site testing of Phytophthora species

Schwenkbier, Lydia; Pollok, Sibyll; König, Stephan; Urban, Matthias; Werres, Sabine; Cialla‐May, Dana; Weber, Karina; Popp, Jürgen

doi:10.1039/c4ay02287d

Cited by 37 publications

(14 citation statements)

References 49 publications

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“…In order to improve the detection and achieve a putative in- field application, novel isothermal amplification techniques could be implemented. 51,52 Moreover, the SERS-based DNA detection, relying on DNA-DNA-hybrid formation, could be performed in a handheld Raman device, that allows the applicability at the point-of-need due to its portability. In summary, a reliable, reproducible and thermally stable SERS assay has been developed for the detection of specific DNA hybridization between immobilized capture probes and the sequence-matching target DNA.…”

Section: Discussionmentioning

confidence: 99%

Label-free detection of Phytophthora ramorum using surface-enhanced Raman spectroscopy

Yüksel

Schwenkbier

Pollok

et al. 2015

Analyst

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In this study, we report on a novel approach for the label-free and species-specific detection of the plant pathogen Phytophthora ramorum from real samples using surface enhanced Raman scattering (SERS). In this context, we consider the entire analysis chain including sample preparation, DNA isolation, amplification and hybridization on SERS substrate-immobilized adenine-free capture probes. Thus, the SERS-based detection of target DNA is verified by the strong spectral feature of adenine which indicates the presence of hybridized target DNA. This property was realized by replacing adenine moieties in the species-specific capture probes with 2-aminopurine. In the case of the matching capture and target sequence, the characteristic adenine peak serves as an indicator for specific DNA hybridization. Altogether, this is the first assay demonstrating the detection of a plant pathogen from an infected plant material by label-free SERS employing DNA hybridization on planar SERS substrates consisting of silver nanoparticles.

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Section: Discussionmentioning

confidence: 99%

Label-free detection of Phytophthora ramorum using surface-enhanced Raman spectroscopy

Yüksel

Schwenkbier

Pollok

et al. 2015

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“…Another advantage of the electrochemical approach is the independence from background light or sample color, even though electrochemically active samples may influence the readout. Examples of portable devices for the electrochemical detection of amplified DNA from plant pathogens are already available (Julich et al, 2011;Schwenkbier et al, 2014Schwenkbier et al, , 2015a. The method exploits the enzymatic-triggered silver deposition on the electrode to read the signal both visually and electrochemically.…”

Section: Detection Methodsmentioning

confidence: 99%

Molecular Approaches for Low-Cost Point-of-Care Pathogen Detection in Agriculture and Forestry

Baldi

Porta

2020

Front. Plant Sci.

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Early detection of plant diseases is a crucial factor to prevent or limit the spread of a rising infection that could cause significant economic loss. Detection test on plant diseases in the laboratory can be laborious, time consuming, expensive, and normally requires specific technical expertise. Moreover, in the developing countries, it is often difficult to find laboratories equipped for this kind of analysis. Therefore, in the past years, a high effort has been made for the development of fast, specific, sensitive, and cost-effective tests that can be successfully used in plant pathology directly in the field by low-specialized personnel using minimal equipment. Nucleic acid-based methods have proven to be a good choice for the development of detection tools in several fields, such as human/animal health, food safety, and water analysis, and their application in plant pathogen detection is becoming more and more common. In the present review, the more recent nucleic acid-based protocols for point-of-care (POC) plant pathogen detection and identification are described and analyzed. All these methods have a high potential for early detection of destructive diseases in agriculture and forestry, they should help make molecular detection for plant pathogens accessible to anyone, anywhere, and at any time. We do not suggest that on-site methods should replace lab testing completely, which remains crucial for more complex researches, such as identification and classification of new pathogens or the study of plant defense mechanisms. Instead, POC analysis can provide a useful, fast, and efficient preliminary on-site screening that is crucial in the struggle against plant pathogens.

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“…Septa allow the sterile injection of fluid samples and reagents, and data were collected through the integration of an electrical readout. A similar approach was used for the detection of selected Phytophthora species [ 88 ], with the development of a helicase-dependent isothermal amplification (HDA) in combination with on-chip hybridization, employing silver nanoparticles as label and enabling both visual detection and electrical readout. The study showed a successful application in amplifying isolated template DNA from Phytophthora cultures and infected plant material.…”

Section: Innovative Technologies For Plant Pathologymentioning

confidence: 99%

Advances in Plant Disease Detection and Monitoring: From Traditional Assays to In-Field Diagnostics

Buja

Sabella

Monteduro

et al. 2021

Sensors

135

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Human activities significantly contribute to worldwide spread of phytopathological adversities. Pathogen-related food losses are today responsible for a reduction in quantity and quality of yield and decrease value and financial returns. As a result, “early detection” in combination with “fast, accurate, and cheap” diagnostics have also become the new mantra in plant pathology, especially for emerging diseases or challenging pathogens that spread thanks to asymptomatic individuals with subtle initial symptoms but are then difficult to face. Furthermore, in a globalized market sensitive to epidemics, innovative tools suitable for field-use represent the new frontier with respect to diagnostic laboratories, ensuring that the instruments and techniques used are suitable for the operational contexts. In this framework, portable systems and interconnection with Internet of Things (IoT) play a pivotal role. Here we review innovative diagnostic methods based on nanotechnologies and new perspectives concerning information and communication technology (ICT) in agriculture, resulting in an improvement in agricultural and rural development and in the ability to revolutionize the concept of “preventive actions”, making the difference in fighting against phytopathogens, all over the world.

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Towards on-site testing of Phytophthora species

Cited by 37 publications

References 49 publications

Label-free detection of Phytophthora ramorum using surface-enhanced Raman spectroscopy

Label-free detection of Phytophthora ramorum using surface-enhanced Raman spectroscopy

Molecular Approaches for Low-Cost Point-of-Care Pathogen Detection in Agriculture and Forestry

Advances in Plant Disease Detection and Monitoring: From Traditional Assays to In-Field Diagnostics

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