Towards quantitative SERS detection of hydrogen cyanide at ppb level for human breath analysis

Lauridsen, Rikke Kragh; Rindzevicius, Tomas; Molin, Søren; Johansen, Helle Krogh; Berg, Rolf W.; Alstrøm, Tommy Sonne; Almdal, Kristoffer; Larsen, Flemming; Schmidt, Michael; Boisen, Anja

doi:10.1016/j.sbsr.2015.07.002

Cited by 43 publications

(33 citation statements)

References 28 publications

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“…After a few hours on the substrate much of the C≡N peak had shifted to 2189 cm −1 because of sufficient amounts and the fact that it had had some time to interact with the Au-coated SERS substrate, forming the stabile [Au(CN) 2 ] − complex, as explained in ref. 19. Figure 3(b) shows the SERS intensity of the cumulated cyanide peaks at 2135 and 2189 cm −1 from 2 until 20 h (ON).…”

Section: Resultsmentioning

confidence: 99%

“…Reprinted with permission from editor of ref. 19). ( b ) The pump (1) induces a vacuum inside the vacuum chamber (2), leading to inflation of the bag (3) and exposure of the SERS substrate (4) to volatiles created by the bacterial culture (5) in the water bath (6).…”

Section: Figurementioning

confidence: 99%

“…An example hereof is using label free Ag colloid SERS to detect single-nucleotide mismatch in short DNA sequences to discover even small nucleotide changes18. We have demonstrated previously that the SERS technique is suitable for cyanide quantification with the estimated lower limit of detection between 1.8 and 18 ppb using highly sensitive gold-coated silicon (Si) nanopillar SERS substrates19. These substrates consist of vertically standing Si nanopillars with gold (Au) caps which can lean against each other for enhanced SERS detection in liquid or gas phase20.…”

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confidence: 99%

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SERS detection of the biomarker hydrogen cyanide from Pseudomonas aeruginosa cultures isolated from cystic fibrosis patients

Lauridsen

Sommer

Johansen

et al. 2017

Sci Rep

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Pseudomonas aeruginosa is the primary cause of chronic airway infections in cystic fibrosis (CF) patients. Persistent infections are seen from the first P. aeruginosa culture in about 75% of young CF patients, and it is important to discover new ways to detect P. aeruginosa at an earlier stage. The P. aeruginosa biomarker hydrogen cyanide (HCN) contains a triple bond, which is utilized in this study because of the resulting characteristic C≡N peak at 2135 cm−1 in a Raman spectrum. The Raman signal was enhanced by surface-enhanced Raman spectroscopy (SERS) on a Au-coated SERS substrate. After long-term infection, a mutation in the patho-adaptive lasR gene can alter the expression of HCN, which is why it is sometimes not possible to detect HCN in the breath of chronically infected patients. Four P. aeruginosa reference strains and 12 clinical P. aeruginosa strains isolated from CF children were evaluated, and HCN was clearly detected from overnight cultures of all wild type-like isolates and half of the later isolates from the same patients. The clinical impact could be that P. aeruginosa infections could be detected at an earlier stage, because daily breath sampling with an immediate output could be possible with a point-of-care SERS device.

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Section: Resultsmentioning

confidence: 99%

Section: Figurementioning

confidence: 99%

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confidence: 99%

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SERS detection of the biomarker hydrogen cyanide from Pseudomonas aeruginosa cultures isolated from cystic fibrosis patients

Lauridsen

Sommer

Johansen

et al. 2017

Sci Rep

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“…Since the signal amplitude varies linearly with the optical excitation power, the performance of the QEPAS sensor can be improved by increasing the excitation laser power. This property differs from other spectra based sensor such as SERS and TDLAS [12,13]. Diode lasers are usually used in QEPAS technique because of their compactness and narrow line width.…”

Section: Selection Of Hcn Absorption Linementioning

confidence: 95%

“…Sensor platforms based on laser spectroscopy such as surface-enhanced Raman spectroscopy (SERS), tunable diode laser absorption spectroscopy (TDLAS) and photoacoustic spectroscopy (PAS) can be used for HCN gas detection. These sensors typically achieve a detection limit of ppm or ppb HCN concentration levels [12][13][14].…”

Section: Introductionmentioning

confidence: 99%

HCN ppt-level detection based on a QEPAS sensor with amplified laser and a miniaturized 3D-printed photoacoustic detection channel

Tong

et al. 2018

Opt. Express

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Ultra-high sensitive and stable detection of hydrogen cyanide (HCN) based on a quartz-enhanced photoacoustic spectroscopy (QEPAS) sensor was realized using an erbium-doped fiber amplifier (EDFA) as well as a miniaturized 3D-printed photoacoustic detection channel (PADC) for the first time. A HCN molecule absorption line located at 6536.46 cm was selected which was in the range of the EDFA emission spectrum. The detection sensitivity of the reported EDFA-QEPAS sensor was enhanced significantly due to the high available EDFA excitation laser power. A 3D printing technique was used to develop the compact PADC, resulting in a size of 29 × 15 × 8 mm and a mass of ~5 g in order to improve the sensor stability and implement sensor applications requiring a compact size and light weight. At atmospheric pressure, room temperature and a 1 s acquisition time, a minimum detection limit (MDL) of 29 parts per billion (ppb) was achieved, corresponding to a normalized noise equivalent absorption (NNEA) coefficient of 1.08 × 10 cmW/Hz. The long-term performance and the stability of the HCN EDFA-QEPAS sensor system were investigated using an Allan deviation analysis. It indicated that the MDL can be improved to 220 parts per trillion (ppt) with an integration time of 300 s, which demonstrated this compact, integrated and miniaturized 3D-printed PADC based sensor had an excellent stability.

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Pressure dependence of the Raman signal intensity in high‐pressure gases

Petrov

Matrosov

2016

J Raman Spectroscopy

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At present gas analysis based on Raman, spectroscopy is actively developed. In most cases, to achieve the required Raman signal intensity, compression of the gas medium is used. However, in comparison with normal conditions, the character of motion of molecules and their electric properties change in high‐pressure gas media, thereby violating a linear dependence of the Raman signal intensity on the concentration of molecules and the gas pressure. In the present work, a theoretical model is presented that describes the Raman signal intensity as a function of the pressure of the gas medium considering the compressibility factor, the internal field factor, and the instrumental factor. To verify the model, changes in the Raman signal intensities of vibrational bands of nitrogen and oxygen in the pressure range 1–80 atm and carbon dioxide in the pressure range 1–60 atm are investigated. Under these conditions, we observe a deviation of ~3% from a linear dependence for nitrogen, ~7% for oxygen and ~80% for carbon dioxide, which is in good agreement with the theoretical model. Raman shifts, half‐widths of Q‐branches of vibrational bands ν1 (and 2ν2 for carbon dioxide) of these molecules and also ratio of integral intensities I(2ν2)/I(ν1) for carbon dioxide under these conditions are investigated. Copyright © 2016 John Wiley & Sons, Ltd.

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Towards quantitative SERS detection of hydrogen cyanide at ppb level for human breath analysis

Cited by 43 publications

References 28 publications

SERS detection of the biomarker hydrogen cyanide from Pseudomonas aeruginosa cultures isolated from cystic fibrosis patients

SERS detection of the biomarker hydrogen cyanide from Pseudomonas aeruginosa cultures isolated from cystic fibrosis patients

HCN ppt-level detection based on a QEPAS sensor with amplified laser and a miniaturized 3D-printed photoacoustic detection channel

Pressure dependence of the Raman signal intensity in high‐pressure gases

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