Towards the DRED of Resin-Supported Combinatorial Libraries: A Non-Invasive Methodology Based on Bead Self-Encoding and Multispectral Imaging

“Smart dust”: nanostructured devices in a grain of sand

“…49,50 An extensive library of codes allows for parallel assays and more rapid screening techniques. Various schemes to incorporate identification codes have been proposed, involving either fluorescent molecules, 51 molecules with specific vibrational signatures, 52,53 quantum dots 54 or discrete metallic layers 55 as the encoding elements. The layered porous Si photonic structures offer some advantages over these other encoding methodologies.…”

Classification of Spectroscopically Encoded Resins by Raman Mapping and Infrared Hyperspectral Imaging

“…To address this challenge, several encoding and deconvolution schemes were reported. Encoding methods benefit from the multitude of microcarriers available, their amenability to split−pool synthesis, and their compatibility with a broad spectrum of encoding/code readout strategies. − The microcarriers can be encoded during library synthesis by adding a detectable chemical tag at each synthesis cycle that encodes for that particular step (parallel encoding approach). Alternatively, the microcarriers can be encoded before the synthesis (pre-encoding approach), − in which case they must be decoded at each synthetic cycle to keep track of their chemical history (directed sorting strategy) .…”

Microbarcode sorting device

“…In encoded bead-based multiplex assays, there are two sources of information, the predefined pattern inside the beads [1][2][3][4][5][6] to identify the type of reaction and the reporter color (e.g., fluorescence) on the surface of the beads to signal the magnitude of the biomolecular reaction. Currently, flow cytometry [7][8][9] and microscopic imaging [1][2][3][4][5][6] are the two commonly used methods to identify and quantify beads. Flow cytometry is well developed for microspheres and there are limited studies on the use of non-spherical shaped beads for flow cytometers.…”