Towards the two-dimensional imaging of spontaneous ultra-weak photon emission from microbial, plant and animal cells

Prasad, Ankush; Pospı́šil, Pavel

doi:10.1038/srep01211

Cited by 52 publications

(38 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is imperative to control any kind of interference from the stray photons and thus a dark room specifically designed for measuring ultra-weak photon emission as described in our previous studies was utilized (Prasad and Pospíšil, 2013). The ultra-weak photon emission imaging was performed using a FIGURE 1 | Spatial distribution of chloroplasts within mesophyll of Arabidopsis leaves kept under diffused green light for 90 min prior to imaging.…”

Section: Measurement Setup and Charge Coupled Device Imagingmentioning

confidence: 99%

“…Other experimental conditions were: spectral sensitivity, 350 to 1,000 nm; readout speed, 100 kHz; gain, 2; and accumulation time: 20 min. All other settings were as in Prasad and Pospíšil (Prasad and Pospíšil, 2013). In order to avoid any kind of intervention of delayed luminescence, the Arabidopsis plant was dark-incubated for approximately 2 h. The mechanical injury was induced using a sharp blade in the presence of diffused green light with precaution not to exert any external mechanical pressure on other parts of the Arabidopsis plant/leaves.…”

Section: Measurement Setup and Charge Coupled Device Imagingmentioning

confidence: 99%

See 1 more Smart Citation

Reactive Oxygen Species as a Response to Wounding: In Vivo Imaging in Arabidopsis thaliana

et al. 2020

Self Cite

View full text Add to dashboard Cite

Mechanical injury or wounding in plants can be attributed to abiotic or/and biotic causes. Subsequent defense responses are either local, i.e. within or in the close vicinity of affected tissue, or systemic, i.e. at distant plant organs. Stress stimuli activate a plethora of early and late reactions, from electric signals induced within seconds upon injury, oxidative burst within minutes, and slightly slower changes in hormone levels or expression of defense-related genes, to later cell wall reinforcement by polysaccharides deposition, or accumulation of proteinase inhibitors and hydrolytic enzymes. In the current study, we focused on the production of reactive oxygen species (ROS) in wounded Arabidopsis leaves. Based on fluorescence imaging, we provide experimental evidence that ROS [superoxide anion radical (O 2 •−) and singlet oxygen (1 O 2)] are produced following wounding. As a consequence, oxidation of biomolecules is induced, predominantly of polyunsaturated fatty acid, which leads to the formation of reactive intermediate products and electronically excited species.

show abstract

Section: Measurement Setup and Charge Coupled Device Imagingmentioning

confidence: 99%

Section: Measurement Setup and Charge Coupled Device Imagingmentioning

confidence: 99%

Reactive Oxygen Species as a Response to Wounding: In Vivo Imaging in Arabidopsis thaliana

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…41 43,31 In further advances, the technique has been expanded to include visualization of spontaneous photon emission in microbial and animal cells, and also live animals and in humans. 44,45 Hence, the technique requires attention to the types of ROS one is trying to measure, and the types of filters used. Importantly, the delayed fluorescence of chlorophyll, and the reaction of radical carbonyl groups with chlorophyll, can cause possible confounding factors in the data analysis and necessitates pre-incubation in the dark to allow for relaxation of the chlorophyll fluorescence.…”

Section: Ultra-weak Photon Emissionmentioning

confidence: 99%

Singlet oxygen detection in biological systems: Uses and limitations

Koh

Fluhr

2016

Plant Signaling & Behavior

View full text Add to dashboard Cite

The study of singlet oxygen in biological systems is challenging in many ways. Singlet oxygen is a relatively unstable ephemeral molecule, and its properties make it highly reactive with many biomolecules, making it difficult to quantify accurately. Several methods have been developed to study this elusive molecule, but most studies thus far have focused on those conditions that produce relatively large amounts of singlet oxygen. However, the need for more sensitive methods is required as one begins to explore the levels of singlet oxygen required in signaling and regulatory processes. Here we discuss the various methods used in the study of singlet oxygen, and outline their uses and limitations.

show abstract

“…EPR spin-trapping spectroscopy is one of the most sensitive and specific method for ROS detection; however, kinetic measurements are not possible at the current stage of development. Chemiluminescence, also known as ultra-weak photon emission, has been widely used recently as a non-invasive method to understand the involvement of ROS in oxidative radical reactions [36][37][38]; however, limitations with respect to the specificity for particular ROS involvement exists [39].…”

Section: Manuscript To Be Reviewedmentioning

confidence: 99%

Peer Review #3 of "Real-time monitoring of superoxide anion radical generation in response to wounding: electrochemical study (v0.1)"

2017

View full text Add to dashboard Cite

Background: The growth and development of plants are deleteriously affected by various biotic and abiotic stress factors. Wounding in plants is caused by exposure to environmental stress, mechanical stress, and via herbivory. Typically, oxidative burst in response to wounding is associated with the formation of reactive oxygen species, such as the superoxide anion radical (O 2 •−), hydrogen peroxide (H 2 O 2) and singlet oxygen; however, few experimental studies have provided direct evidence of their detection in plants. Detection of O 2 •− formation in plant tissues have been performed using various techniques including electron paramagnetic resonance spin-trap spectroscopy, epinephrineadrenochrome acceptor methods, staining with dyes such as tetrazolium dye and nitro blue tetrazolium (NBT), however, kinetic measurements have not been performed. In the current study, we provide evidence of O 2 •− generation and its kinetics in the leaves of spinach (Spinacia oleracea) subjected to wounding. Methods: Real-time monitoring of O 2 •− generation was performed using catalytic amperometry. Changes in oxidation current for O 2 •− was monitored using polymeric iron-porphyrin-based modified carbon electrodes (φ=1 mm) as working electrode with Ag/AgCl as the reference electrode. Result: The results obtained show a continuous generation of generation of O 2 •− for minutes after wounding, followed by a decline. The exogenous addition of superoxide dismutase, which is known to dismutate O 2 •− to H 2 O 2 , significantly suppressed the oxidation current. Conclusion: Catalytic amperometric measurements were performed using polymeric iron-porphyrin based modified carbon electrode. It is claimed to be a useful tool and a direct method for real-time monitoring and precise detection of O 2 •− in biological samples. It is claimed to bear a potential for its wide application in plant research for specific and sensitive detection of O 2 •− .

show abstract

Towards the two-dimensional imaging of spontaneous ultra-weak photon emission from microbial, plant and animal cells

Cited by 52 publications

References 27 publications

Reactive Oxygen Species as a Response to Wounding: In Vivo Imaging in Arabidopsis thaliana

Reactive Oxygen Species as a Response to Wounding: In Vivo Imaging in Arabidopsis thaliana

Singlet oxygen detection in biological systems: Uses and limitations

Peer Review #3 of "Real-time monitoring of superoxide anion radical generation in response to wounding: electrochemical study (v0.1)"

Contact Info

Product

Resources

About