Towards the understanding of the UV light, riboflavin and additive solution contributions to the in vitro lesions observed in Mirasol®-treated platelets

Abonnenc, Mélanie; Crettaz, David; Sonego, Giona; Escolar, Ginés; Tissot, Jean‐Daniel; Prudent, Michel

doi:10.1016/j.tracli.2019.09.001

Cited by 9 publications

(27 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The percentage of HSR slightly decreases during the storage, which is further exacerbated by pathogen-inactivation treatments. 21,22 It negatively correlates to the PS exposure in conventional storage. 22 HSR is an easy-to-use technique and it might be taken into account to assess the level of lesions at the cellular level.…”

Section: Function Of Stored Plateletsmentioning

confidence: 99%

“…GPIb (also called CD42b, part of the von Willebrand receptor) decreases during the storage until day 7 postdonation and its expression is influenced by treatments such as pathogen inactivation. [21][22][23][24][25] Indeed, amotosalen/UV-A treatment induces the desialylation and cleavage of this receptor (increase of glycocalicin). 24 GPIIbIIIa (integrin αIIbßIII, fibrinogen receptor), GPIaIIa (integrin α2β1, collagen receptor), and GPVI (immunoglobulin-like transmembrane protein, collagen receptor) remain stable through the storage.…”

Section: Phenotype Of Stored Plateletsmentioning

confidence: 99%

“…An increase activation of this GP has been reported following pathogen-inactivation procedures (higher in the case of riboflavin/UV light). 21,22 Of note, the treatments used do not provide the same levels of activation.…”

Section: Phenotype Of Stored Plateletsmentioning

confidence: 99%

“…It increases after processing steps as well during the storage. [21][22][23][25][26][27] As for the PS exposure (usually quantified by binding to annexin V), the percentages increase and the effect is even exacerbated by the pathogen inactivation. It has to be noticed that the sample preparation influences the results.…”

Section: Phenotype Of Stored Plateletsmentioning

confidence: 99%

“…4 The use of a couple of agonists to activate platelets (e.g., epinephrine and ADP or collagen) in vitro induces a higher response than single activation. 22 Aggregometry allows us to probe different pathways in platelet aggregation, which is of interest in the preparation of PCs. The different centrifugation steps or the pathogen inactivation modify the response to agonists, which might provide information on the impacted molecular pathways.…”

Section: Function Of Stored Plateletsmentioning

confidence: 99%

See 4 more Smart Citations

What about Platelet Function in Platelet Concentrates?

Prudent

2020

Hamostaseologie

View full text Add to dashboard Cite

The characterization of platelet concentrates (PCs) in transfusion medicine has been performed with different analytical methods and platelet lesions (from biochemistry to cell biology) have been documented. In routine quality assessment and validation of manufacturing processes of PCs for transfusion purposes, only basic parameters are monitored and the platelet functions are not included. However, PCs undergo several manipulations during the processing and the basic parameters do not provide sensitive analyses to properly picture out the impact of the blood component preparation and storage on platelets. To improve the transfusion supply chain and the platelet functionalities, additional parameters should be used. The present short review will focus on the different techniques to monitor ex vivo platelet lesions from phenotype characterization to advanced omic analyses. Then, the opportunities to use these methods in quality control, process validation, development, and research will be discussed. Functional markers should be considered because they would be an advantage for the future developments in transfusion medicine.

show abstract

Section: Function Of Stored Plateletsmentioning

confidence: 99%

Section: Phenotype Of Stored Plateletsmentioning

confidence: 99%

Section: Phenotype Of Stored Plateletsmentioning

confidence: 99%

Section: Phenotype Of Stored Plateletsmentioning

confidence: 99%

Section: Function Of Stored Plateletsmentioning

confidence: 99%

See 3 more Smart Citations

What about Platelet Function in Platelet Concentrates?

Prudent

2020

Hamostaseologie

View full text Add to dashboard Cite

show abstract

Pathogen Reduction Technologies and Their Impact on Metabolic and Functional Properties of Treated Platelet Concentrates: A Systematic Review

Tsalas

Petrou

Tsantes

et al. 2022

Semin Thromb Hemost

View full text Add to dashboard Cite

Pathogen reduction technologies (PRTs) such as Mirasol and Intercept were developed to eliminate transfusion-transmitted infections. The impact of PRTs on platelet function during the storage period, their effect on platelet storage lesions, and the optimal storage duration following PRTs have not been clearly defined. The aim of this study was to systematically review the existing literature and investigate the impact of PRTs on functional alterations of PRT-treated platelets during the storage period. The authors identified 68 studies suitable to be included in this review. Despite the high heterogeneity in the literature, the results of the published studies indicate that PRTs may increase platelet metabolic activity, accelerate cell apoptosis, and enhance platelet activation, which can subsequently lead to a late exhaustion of activation potential and reduced aggregation response. However, these effects have a minor impact on platelet function during the early storage period and become more prominent beyond the fifth day of the storage period. Large in vivo trials are required to evaluate the effectiveness of PRT-treated platelets during the storage period and investigate whether their storage can be safely extended to more than 5 days, and up to the traditional 7-day storage period.

show abstract

Sulfenylome analysis of pathogen‐inactivated platelets reveals the presence of cysteine oxidation in integrin signaling pathway and cytoskeleton regulation

Sonego

Crettaz

et al. 2021

Journal of Thrombosis and Haemostasis

View full text Add to dashboard Cite

Cysteine oxidation to sulfenic acid plays a key role in redox regulation and signal transduction. Platelet sulfenylome was studied by quantitative proteomics in pathogen inactivated platelets. One hundred and seventy‐four sulfenylated proteins were identified in resting platelets. Pathogen inactivation oxidized integrin βIII, which could activate the mitogen‐activated protein kinases pathway. Abstract BackgroundCysteine‐containing protein modifications are involved in numerous biological processes such redox regulation or signal transduction. During the preparation and storage of platelet concentrates, cell functions and protein regulations are impacted. In spite of several proteomic investigations, the platelet sulfenylome, ie, the proteins containing cysteine residues (R‐SH) oxidized to sulfenic acid (R‐SOH), has not been characterized. MethodsA dimedone‐based sulfenic acid tagging and enrichment coupled to a mass spectrometry identification workflow was developed to identify and quantify the sulfenic acid‐containing proteins in platelet concentrates treated or not with an amotosalen/ultraviolet A (UVA) pathogen inactivation technique. ResultsOne hundred and seventy‐four sulfenylated proteins were identified belonging mainly to the integrin signal pathway and cytoskeletal regulation by Rho GTPase. The impact on pathogen inactivated platelet concentrates was weak compared to untreated ones where three sulfenylated proteins (myosin heavy chain 9, integrin βIII, and transgelin 2) were significantly affected by amotosalen/UVA treatment. Of particular interest, the reported oxidation of cysteine residues in integrin βIII is known to activate the receptor αIIbβIII. Following the pathogen inactivation, it might trigger the phosphorylation of p38MAPK and explain the lesions reported in the literature. Moreover, procaspase activating compound‐1 (PAC‐1) binding assays on platelet activation showed an increased response to adenosine diphosphate exacerbated by the tagging of proteins with dimedone. This result corroborates the hypothesis of an oxidation‐triggered activation of αIIbβIII by the pathogen inactivation treatment. ConclusionsThe present work completes missing information on the platelet proteome and provides new insights on the effect of pathogen inactivation linked to integrin signaling and cytoskeleton regulation.

show abstract

Towards the understanding of the UV light, riboflavin and additive solution contributions to the in vitro lesions observed in Mirasol®-treated platelets

Cited by 9 publications

References 41 publications

What about Platelet Function in Platelet Concentrates?

What about Platelet Function in Platelet Concentrates?

Pathogen Reduction Technologies and Their Impact on Metabolic and Functional Properties of Treated Platelet Concentrates: A Systematic Review

Sulfenylome analysis of pathogen‐inactivated platelets reveals the presence of cysteine oxidation in integrin signaling pathway and cytoskeleton regulation

Contact Info

Product

Resources

About