Toxic Effect of a Marine Bacterium on Aquatic Organisms and Its Algicidal Substances against Phaeocystis globosa

Yang, Qiuchan; Chen, Lina; Hu, Xiaoli; Zhao, Liancheng; Yin, Ping; Li, Qiang

doi:10.1371/journal.pone.0114933

Cited by 17 publications

(7 citation statements)

References 43 publications

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“…Those exceptions present some interesting biological characteristics. For example, several Bacillus strains exhibit strong killing activity against the marine algae Phaeocystis globose , Cochlodinium polykrikoides (now renamed Margalefidinium polykrikoides ) or Chattonella marina (Jeong et al, 2003; Kim et al, 2009; Yang et al, 2015). A strain of Streptomyces iranensis can kill the green alga Chlamydomonas reinhardtii by means of its metabolic product azalomycin F, whilst this algicidal activity can be eliminated by a lichen‐like association between Aspergillus nidulans and C. reinhardtii (Krespach et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Algicidal interaction between Paenibacillus polymyxa MEZ6 and microalgae

Zhao

Ren

et al. 2022

Journal of Applied Microbiology

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Aims: Algicidal bacteria can be used for control of harmful algal bloom and extraction of algal bioproducts based on their algae-lysing activities. This work investigated the algae-lysing activity of a newly isolated algicidal bacterium, Paenibacillus polymyxa strain MEZ6 and its possible mechanisms. Methods and Results: Algicidal bacteria were isolated from soil samples collected at the university campus. Co-inoculation tests identified that one isolate, MEZ6, can rapidly kill eukaryotic algae including Chlamydomonas reinhardtii, Tribonema minus, Haematococcus pluvialis, and Chlorella ellipsoidea. The strain was determined as Paenibacillus polymyxa MEZ6 based on 16S rRNA gene sequence and genome comparisons. The algicidal activity was detected in both living cells and cell-free supernatant of spent culture medium, suggesting cell-cell contact is not required for algicidal activity. Strain MEZ6 was less active towards cyanobacterial strains compared to algae. Genomic sequence and comparative proteomic analyses were performed to explore the possible algicidal mechanisms of the strain. Differentially expressed protein analysis identified a number of proteins related to polysaccharides degradation and antimicrobial secondary metabolite biosynthesis that may be involved in the algicidal activity of MEZ6. Conclusion: Paenibacillus polymyxa MEZ6 is a newly discovered gram-positive algicidal bacterial strain with high lytic activity towards several algal species. Significance and Impact of the Study: Our study extends the understanding of the versatile characters of Paenibacillus polymyxa and sheds new insights into its application in algae biotechnology.

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Section: Introductionmentioning

confidence: 99%

Algicidal interaction between Paenibacillus polymyxa MEZ6 and microalgae

Zhao

Ren

et al. 2022

Journal of Applied Microbiology

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“…Considerable efforts have been devoted to the investigation of environmental conditions that regulate the occurrence and maintenance of P. donghaiense blooms and the cellular responses of this dinoflagellate to different conditions, such as N/P repletion, N/P depletion, different N/P substrates, acidification, and eutrophication (25)(26)(27)(28)(29). Although many algicidal bacteria have been identified and exploited in the control of Microcystis aeruginosa (5,7,14,17,30), Alexandrium tamarense (9,12,18,19), Phaeocystis globosa (8,11,16,(31)(32)(33), and Skeletonema costatum (34)(35)(36)(37), few studies have reported bacteria with algicidal effects against P. donghaiense. Therefore, an effective biocontrol method for the prevention and dissolution of P. donghaiense algal blooms must be developed.…”

mentioning

confidence: 99%

Algicidal Activity of Novel Marine Bacterium Paracoccus sp. Strain Y42 against a Harmful Algal-Bloom-Causing Dinoflagellate, Prorocentrum donghaiense

Zhang

Chen

et al. 2018

Appl Environ Microbiol

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blooms occur frequently in the Yangtze River estuary and the adjacent East China Sea. These blooms have damaged marine ecosystems and caused enormous economic losses over the past 2 decades. Thus, highly efficient, low-cost, ecofriendly approaches must be developed to control blooms. In this study, a bacterial strain (strain Y42) was identified as sp. and was used to lyse The supernatant of the strain Y42 culture was able to lyse, and the algicidal activity of this Y42 supernatant was stable with different temperatures and durations of light exposure and over a wide pH range. In addition to , Y42 showed high algicidal activity against, , and, suggesting that it targets primarily Pyrrophyta. To clarify the algicidal effects of Y42, we assessed algal lysis and determined the chlorophyll contents, photosynthetic activity, and malondialdehyde contents of after exposure to the Y42 supernatant. Scanning electron microscopy and transmission electron microscopy analyses showed that the Y42 supernatant disrupted membrane integrity and caused algal cell breakage at the megacytic zone. Photosynthetic pigment loss and significant declines in both photosynthetic efficiency and the electron transport rate indicated that the Y42 supernatant damaged the photosynthetic system of Malondialdehyde overproduction indicated that the Y42 supernatant caused lipid peroxidation and oxidative damage to membrane systems in the algal cell, ultimately leading to death. The findings of this study reveal the potential of Y42 to remove algal cells from blooms. is one of the most common dinoflagellate species that form harmful algal blooms, which frequently cause serious ecological pollution and pose health hazards to humans and other animals. Screening for bacteria with high algicidal activity against and studying their algicidal processes and characteristics will contribute to an understanding of their algicidal effects and provide a theoretical basis for preventing algal blooms and reducing their harm to the environment. This study reports the algicidal activity and characteristics of against The stability of the algicidal activity of in different environments (including different temperature, pH, and sunlight conditions) indicates its potential for use in the control of blooms.

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“…DNA of the isolates was extracted by using the CTAB method (Wilson, 2003). The 16S rDNA fragment was amplified by PCR (polymerase chain reaction) using primers 27f(5'-GAGAGTTTGATCCTGGCTCAG-3') and 1495r(5'-CTACGGCTACCTTGTTACGA-3') (Yang et al, 2015;Arimurti et al, 2017) employing 40 cycles of the following program: denaturation at 95 °C for 20 sec, annealing at 55 °C for 30 sec, and an elongation at 72 °C for 90 sec. Finally, it was followed by a final elongation at 72 °C for 5 min was performed.…”

Section: Identification and Biochemical Analysis Of Selected Caffeine-degrading Bacteriamentioning

confidence: 99%

Degradation of caffeine by Pseudomonas monteilii KRM9

Arimurti¹,

Ardyati²,

Nurani³

et al. 2018

MJM

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Aims:The objective of this research was to isolate caffeine-degrading bacteria from coffee pulp waste in Indonesia and characterize their caffeine degradation activity. Methodology and results: The caffeine-degrading bacteria were isolated from coffee pulp wastes of Coffea arabica and C. canephora. These isolates were selected based on their caffeine degradation activity. The identification and biochemical properties of the best isolate were conducted via 16S rDNA sequence analyses and by using the Microbact kit. Meanwhile, caffeine degradation activity of this bacteria was analyzed by using LC-MS/MS. The results indicated that fourteen bacterial isolates were able to degrade caffeine. The highest caffeine degradation activity was performe d by isolate KRM9 at the rate of 99.26 ± 0.01%, on a caffeine medium after 24 h of incubation. Based on the 16S rDNA analyses, the KRM9 isolate was identified as Pseudomonas monteilii. Till present, this species has not been reported as a caffeine-degrading bacterium. However, LC-MS/MS analysis indicated that caffeine was degraded by P. monteilii KRM9 and theobromine was not the secondary metabolite of caffeine degradation. Conclusion, significance and impact of study: Pseudomonas monteilii KRM9 was detected as a new isolate of caffeine-degrading bacteria. This bacterium can be introduced as an agent to degrade caffeine from coffee pulp waste. It is expected that further research can be conducted on the overall mechanism of caffeine degradation by P. monteilii KRM9.

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Toxic Effect of a Marine Bacterium on Aquatic Organisms and Its Algicidal Substances against Phaeocystis globosa

Cited by 17 publications

References 43 publications

Algicidal interaction between Paenibacillus polymyxa MEZ6 and microalgae

Algicidal interaction between Paenibacillus polymyxa MEZ6 and microalgae

Algicidal Activity of Novel Marine Bacterium Paracoccus sp. Strain Y42 against a Harmful Algal-Bloom-Causing Dinoflagellate, Prorocentrum donghaiense

Degradation of caffeine by Pseudomonas monteilii KRM9

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