Toxic effects of copper sulfate on diploid and triploid fin cell lines in Misgurnus anguillicaudatus

Qin, Yanjie; Li, Xia; Yang, Yanjin; Li, Zhuangzhuang; Liang, Yan; Zhang, Xiaoyu; Jiang, Shan

doi:10.1016/j.scitotenv.2018.06.315

Cited by 13 publications

(6 citation statements)

References 62 publications

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“…Meanwhile, ROS are produced by xanthine dehydrogenase/oxidase to counter the free radicals produced in the body (Xiao et al, 2021). Qin et al (2018) found that DNA breaks were observed in Misgurnus anguillicaudatus after exposure to 400 and 800 μM concentrations of copper sulphate for 24 h. Tetrahymena thermophila produced a severe dose‐dependent DNA damage response under koumine stress of 0.8 mg/mL. In our experiment, a decrease in deoxyguanosine content suggests that the DNA of C. irritants tomonts may be damaged and deoxyguanosine may be oxidized and reduced.…”

Section: Discussionmentioning

confidence: 45%

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Effect of copper sulphate on Cryptocaryon irritans based on metabolome analysis

Zhan

Miao

Zhao

et al. 2023

Journal of Fish Diseases

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Cryptocaryon irritans is one of the most harmful marine parasites in mariculture.Copper sulphate is often used to kill parasites and the influence of copper sulphate on the tomont stage of C. irritans was explored in this study. The results showed that excystment rate was not significantly affected when tomonts were exposed to 5 mg/L (76.7%) and 10 mg/L (78.9%) of copper sulphate for 3 h. However, excystment rate was significantly inhibited when exposed to 15 mg/L (33.3%) for 3 h and 5 mg/L (28.9%), 10 mg/L (33.3%) and 15 mg/L (33.3%) for 6 h. After treatment with high concentrations of copper sulphate, the interior of the tomonts was fuzzy under the microscope, and the division process could not be observed. Metabolomic results combined with preliminary transcriptome analysis results showed that the tomonts were induced to produce linoleate, riboflavin, inositol and other substances under the stress of Cu 2+ , which affected the antioxidant mechanism of the body. Using MDA content determination and antioxidant enzyme activity analysis, copper sulphate was found to cause oxidative damage to tomonts by affecting the generation of metabolites, leading to the death of tomonts.

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Section: Discussionmentioning

confidence: 45%

Section: Discussionmentioning

confidence: 99%

Effect of copper sulphate on Cryptocaryon irritans based on metabolome analysis

Zhan

Miao

Zhao

et al. 2023

Journal of Fish Diseases

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“…Meanwhile, SOD was involved in the elimination of ROS produced by metal ions [17,34]. However, when the concentrations of Cd 2+ and Cu 2+ were high beyond the capacity of these enzymes, severe cell death and reduction of these enzymes were found [35]. In this respect, the elevation of GSH and SOD could be considered as the recovering of self-protective Fig.…”

Section: Discussionmentioning

confidence: 99%

The protective effects of glycyrrhizic acid on heavy metal ions-induced reproductive toxicity in Chinese hamster ovary cells

Gao¹,

Liu²,

Du³

et al. 2021

ScienceAsia

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In this work, Chinese hamster ovary (CHO) cells were used to examine the protective effects of glycyrrhizic acid (GA) on the reproductive toxicity of heavy metal ions, including Cd 2+ and Cu 2+ . As a result, both metal ions induced significant toxicity in CHO cells after 48-h treatment, as revealed by a severe decrease in cell viability. GA and glutathione (GSH) largely reduced the toxicity caused by Cd 2+ and Cu 2+ , which could be due to their recovery of GSH levels and superoxide dismutase (SOD) activities in CHO cells. In addition, GA and GSH significantly up-regulated the gene expressions of glutathione S-transferase (gst), sod, and heme oxygenase (ho)-1, which could be another explanation for their protective effects. More importantly, GA exhibited comparable protective effects as GSH but at much lower concentrations (50-100 µM v.s. 500-1000 µM). Therefore, GA could be effective for the alleviation of reproductive toxicity of Cd 2+ /Cu 2+ , which needs further investigation in animal models.

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“…In a sub-individual level, M. anguillicaudatus indicated hepatic damage evidenced by transaminase activity decline in liver, swelled shape and arranged loose in hepatocyte by exposure of dichlorvos, wastewater contaminated with PBDE, metals [ 83 , 86 ]. In genetic toxicity analysis, dichlorvos, flufiprole, copper sulfate, mitomycin C and Trichloroethylene caused DNA strand break to the liver cell, chromosomal aberration increase, micronucleus rate increase [ 33 , 81 , 82 , 86 ]. In response to progesterone, phenanthrene, 17β-estradiol (E2), 17α-ethinylestradiol (EE2), flufiprole, Venlafaxine (VFX), o-desmethylvenlafaxine (OVFX), glyphosate, H 2 O 2 , M. anguillicaudatus exhibited significant changes of endocrine disruption and development process disturbance, oxidative stress, neurotoxicity, cellular mortality, cellular structure changes, apoptosis occurrence [ 33 , 82 , 87 , 88 , 89 , 90 , 91 ].…”

Section: Mining Promising Test Speciesmentioning

confidence: 99%

“…Genetically, they have diverse polyploidy population from 2n to 6n [30]. Triploid cell lines were considered to have superior flesh than diploid, attracting industrial attention [33].…”

Section: Life Cycle and Physiological Characteristicsmentioning

confidence: 99%

Proposing Effective Ecotoxicity Test Species for Chemical Safety Assessment in East Asia: A Review

Lee,

Shim,

Park

2023

Toxics

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East Asia leads the global chemical industry, but environmental chemical risk in these countries is an emerging concern. Despite this, only a few native species that are representative of East Asian environments are listed as test species in international guidelines compared with those native to Europe and America. This review suggests that Zacco platypus, Misgurnus anguillicaudatus, Hydrilla verticillata, Neocaridina denticulata spp., and Scenedesmus obliquus, all resident to East Asia, are promising test species for ecotoxicity tests. The utility of these five species in environmental risk assessment (ERA) varies depending on their individual traits and the state of ecotoxicity research, indicating a need for different applications of each species according to ERA objectives. Furthermore, the traits of these five species can complement each other when assessing chemical effects under diverse exposure scenarios, suggesting they can form a versatile battery for ERA. This review also analyzes recent trends in ecotoxicity studies and proposes emerging research issues, such as the application of alternative test methods, comparative studies using model species, the identification of specific markers for test species, and performance of toxicity tests under environmentally relevant conditions. The information provided on the utility of the five species and alternative issues in toxicity tests could assist in selecting test species suited to study objectives for more effective ERA.

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Toxic effects of copper sulfate on diploid and triploid fin cell lines in Misgurnus anguillicaudatus

Cited by 13 publications

References 62 publications

Effect of copper sulphate on Cryptocaryon irritans based on metabolome analysis

Effect of copper sulphate on Cryptocaryon irritans based on metabolome analysis

The protective effects of glycyrrhizic acid on heavy metal ions-induced reproductive toxicity in Chinese hamster ovary cells

Proposing Effective Ecotoxicity Test Species for Chemical Safety Assessment in East Asia: A Review

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