Toxic effects of dietary exposure to T-2 toxin on intestinal and hepatic biotransformation enzymes and drug transporter systems in broiler chickens

Osselaere, Ann; Li, S.J.; Bock, Lies De; Devreese, Mathias; Goossens, Joline; Vandenbroucke, Virginie; Bocxlaer, Jan Van; Boussery, Koen; Pasmans, Frank; Martel, An; Backer, Patrick De; Croubels, Siska

doi:10.1016/j.fct.2012.12.055

Cited by 35 publications

(26 citation statements)

References 43 publications

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“…HPLC/MS was also used to measure the toxicokinetic parameters of DON in broilers and in turkey poults. In broilers, DON was administered in the crop (0.75 mg/kg BW), with no feed supplied for a period of 6 h before and 4 h after administration of the toxin [ 23 ]. In turkey poults, DON was solubilized in ethanol and water and administered (0.75 mg/kg BW) after a 12 h fast [ 24 ].…”

Section: Deoxynivalenolmentioning

confidence: 99%

“…In turkey poults, DON was solubilized in ethanol and water and administered (0.75 mg/kg BW) after a 12 h fast [ 24 ]. T max was measured 0.6 h after administration in both species [ 23 , 24 ]. In the broilers, T 1/2elim was 0.63, and in the turkey poults, T 1/2elim was 0.86 h. Absolute oral bioavailability of DON was around 20% in both species.…”

Section: Deoxynivalenolmentioning

confidence: 99%

“…In the broilers, T 1/2elim was 0.63, and in the turkey poults, T 1/2elim was 0.86 h. Absolute oral bioavailability of DON was around 20% in both species. In both studies conducted with DON administered solubilized in the crop, T max was observed earlier and T 1/2elim was shorter than when radiolabelled DON was added to the feed, suggesting that the procedure used for the administration of the toxin is responsible for these differences [ 15 , 16 , 23 , 24 ]. Whereas the comparison of T max , T 1/2elim and F measured for DON in broilers and turkey poults revealed no difference, the metabolism of the toxin showed some differences.…”

Section: Deoxynivalenolmentioning

confidence: 99%

“…When T-2 toxin was administered directly in the crop of broilers at 0.02 mg/kg BW with no feed supplied for a period of 6 h before and 4 h after administration of the toxin, no trace of T-2 or HT-2 (LOD < 1 ng/mL) was found in plasma [ 23 ]. When T-2 toxin was administered by the intravenous route, very rapid elimination occurred (T 1/2el = 3.9 min) and HT-2 was detected in plasma only 2 min after administration [ 23 ]. Other toxicokinetic investigations in broilers were performed using higher doses of T-2 toxin [ 41 ].…”

Section: T2 Toxinmentioning

confidence: 99%

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Fusariotoxins in Avian Species: Toxicokinetics, Metabolism and Persistence in Tissues

Guerre

2015

Toxins

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Fusariotoxins are mycotoxins produced by different species of the genus Fusarium whose occurrence and toxicity vary considerably. Despite the fact avian species are highly exposed to fusariotoxins, the avian species are considered as resistant to their toxic effects, partly because of low absorption and rapid elimination, thereby reducing the risk of persistence of residues in tissues destined for human consumption. This review focuses on the main fusariotoxins deoxynivalenol, T-2 and HT-2 toxins, zearalenone and fumonisin B1 and B2. The key parameters used in the toxicokinetic studies are presented along with the factors responsible for their variations. Then, each toxin is analyzed separately. Results of studies conducted with radiolabelled toxins are compared with the more recent data obtained with HPLC/MS-MS detection. The metabolic pathways of deoxynivalenol, T-2 toxin, and zearalenone are described, with attention paid to the differences among the avian species. Although no metabolite of fumonisins has been reported in avian species, some differences in toxicokinetics have been observed. All the data reviewed suggest that the toxicokinetics of fusariotoxins in avian species differs from those in mammals, and that variations among the avian species themselves should be assessed.

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Section: Deoxynivalenolmentioning

confidence: 99%

Section: Deoxynivalenolmentioning

confidence: 99%

Section: Deoxynivalenolmentioning

confidence: 99%

Section: T2 Toxinmentioning

confidence: 99%

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Fusariotoxins in Avian Species: Toxicokinetics, Metabolism and Persistence in Tissues

Guerre

2015

Toxins

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“…Osselaere et al . [ 11 ] demonstrated significant down-regulation of CYP1A4, CYP1A5 and CYP3A37 mRNA expression when broilers were fed a diet contaminated with T-2 toxin.…”

Section: Introductionmentioning

confidence: 99%

The roles of carboxylesterase and CYP isozymes on the in vitro metabolism of T-2 toxin

Lin

Chen

et al. 2015

Military Med Res

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BackgroundT-2 toxin poses a great threat to human health because it has the highest toxicity of the currently known trichothecene mycotoxins. To understand the in vivo toxicity and transformation mechanism of T-2 toxin, we investigated the role of one kind of principal phase I drug-metabolizing enzymes (cytochrome P450 [CYP450] enzymes) on the metabolism of T-2 toxin, which are crucial to the metabolism of endogenous substances and xenobiotics. We also investigated carboxylesterase, which also plays an important role in the metabolism of toxic substances.MethodsA chemical inhibition method and a recombinant method were employed to investigate the metabolism of the T-2 toxin by the CYP450 enzymes, and a chemical inhibition method was used to study carboxylesterase metabolism. Samples incubated with human liver microsomes were analyzed by high performance liquid chromatography-triple quadrupole mass spectrometry (HPLC- QqQ MS) after a simple pretreatment.ResultsIn the presence of a carboxylesterase inhibitor, only 20 % T-2 toxin was metabolized. When CYP enzyme inhibitors and a carboxylesterase inhibitor were both present, only 3 % of the T-2 toxin was metabolized. The contributions of the CYP450 enzyme family to T-2 toxin metabolism followed the descending order CYP3A4, CYP2E1, CYP1A2, CYP2B6 or CYP2D6 or CYP2C19.ConclusionCarboxylesterase and CYP450 enzymes are of great importance in T-2 toxin metabolism, in which carboxylesterase is predominant and CYP450 has a subordinate role. CYP3A4 is the principal member of the CYP450 enzyme family responsible for T-2 toxin metabolism. The primary metabolite produced by carboxylesterase is HT-2, and the main metabolite produced by CYP 3A4 is 3′-OH T-2. The different metabolites show different toxicities. Our results will provide useful data concerning the toxic mechanism, the safety evaluation, and the health risk assessment of T-2 toxin.

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Polycarbonate Plastics and Neurological Disorders: From Exposure to Preventive Interventions

Anwar

Anjum

Ghayas

2021

Emerging Contaminants and Associated Treatment Technologies

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Toxic effects of dietary exposure to T-2 toxin on intestinal and hepatic biotransformation enzymes and drug transporter systems in broiler chickens

Cited by 35 publications

References 43 publications

Fusariotoxins in Avian Species: Toxicokinetics, Metabolism and Persistence in Tissues

Fusariotoxins in Avian Species: Toxicokinetics, Metabolism and Persistence in Tissues

The roles of carboxylesterase and CYP isozymes on the in vitro metabolism of T-2 toxin

Polycarbonate Plastics and Neurological Disorders: From Exposure to Preventive Interventions

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