Toxic effects of different-sized graphene oxide particles on zebrafish embryonic development

Chen, Zhong; Chen, Yu; Khan, Imran Ahamed; Tang, Yi; Liu, Shuai; Yang, Ming

doi:10.1016/j.ecoenv.2020.110608

Cited by 50 publications

(33 citation statements)

References 45 publications

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“…14,58,59 Upon exposure of NanoGO to the embryo, it is quickly excreted out, causing it to take a longer time to accumulate in the embryo and induce toxic effects as elucidated by the delayed toxicity profile of NanoGO shown in Figure 8C. Large size GO with sharp edges interacts with the cells membrane and adhere to the embryonic chorion, 60 which in turns exacerbate the impacts on survival of the cells at the highest exposure dose, while small size GO induced significant cytotoxicity via internalization into the cells even at the lower concentration exposed. 61 There was a significant improvement of the survival rate of the treated embryos when exposed to NanoGO-PF at similar concentrations (1-100 µg/mL) (Figure 8D).…”

mentioning

confidence: 99%

Synthesis, Characterization, and Toxicity Assessment of Pluronic F127-Functionalized Graphene Oxide on the Embryonic Development of Zebrafish (Danio rerio)

Shamsi

Alagan

Sarchio

et al. 2020

IJN

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Background In the current literature, there are ongoing debates on the toxicity of graphene oxide (GO) that demonstrate contradictory findings regarding its toxicity profile. As a potential drug carrier, these findings are very concerning due to the safety concerns in humans, as well as the dramatic rise of GO being excreted into the environment. Therefore, there is an imperative need to mitigate the potential toxicity of GO to allow for a safer application in the future. Purpose The present study aims to address this issue by functionalizing GO with Pluronic F127 (PF) as a means to mitigate toxicity and resolve the biocompatibility of GO. Although results from previous studies generally indicated that Pluronic functionalized GO exhibits relatively low toxicity to living organisms, reports that emphasize on its toxicity, particularly during embryonic developmental stage, are still scarce. Methods In the present study, two different sizes of native GO samples, GO and NanoGO, as well as PF-functionalized GO, GO-PF and NanoGO-PF, were prepared and characterized using DLS, UV-Vis, Raman spectroscopy, FTIR, and FESEM analyses. Toxicological assessment of all GO samples (0–100 µg/mL) on zebrafish embryonic developmental stages (survival, hatching and heart rates, and morphological changes) was recorded daily for up to 96 hours post-fertilization (hpf). Results The toxicity effects of each GO sample were observed to be higher at increasing concentrations and upon prolonged exposure. NanoGO demonstrated lower toxicity effects compared to GO. GO-PF and NanoGO-PF were also found to have lower toxicity effects compared to native GO samples. GO-PF showed the lowest toxicity response on zebrafish embryo. Conclusion These findings highlight that toxicity is dependent on the concentration, size, and exposure period of GO. Functionalization of GO with PF through surface coating could potentially mitigate the toxicity effects of GO in embryonic developmental stages, but further investigation is warranted for broader future applications.

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mentioning

confidence: 99%

Synthesis, Characterization, and Toxicity Assessment of Pluronic F127-Functionalized Graphene Oxide on the Embryonic Development of Zebrafish (Danio rerio)

Shamsi

Alagan

Sarchio

et al. 2020

IJN

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“…This was also exhibited on observed endpoints of delay in hatching time, shortened body length, modification in heart rate and blood flow, response in photoperiod stimulation, enhanced activities of superoxide dismutase, AChE, caspase-3, and induction of apoptosis-related genes expression. Specifically, oxidative stress and induction of apoptosis in all three different sized GO particles predicted a potential risk of GO on marine organisms [103]. The group of Li reported that when corannulene (non-planar PAH) and graphene (planar PAH) were exposed to zebrafish larvae at 1, 10, and 50 µg/mL, minimal developmental toxicity and significant decrease in locomotion/increase in sleep caused by corannulene with no significant locomotion alterations at 50 µg/mL graphene were observed.…”

Section: Exposure Accumulation and Bio-distribution Of Graphene In mentioning

confidence: 97%

“…GO particles (50-200 nm, <500 nm, and >500 nm) at 0.1, 1, 10, and 100 mg/L for 4-124 h post-fertilization [103] Danio rerio Microinjection (4 nL/embryo) Graphene induced no significant locomotion alterations, sleep behavior, and gene expression patterns. Graphene at 1, 10, 50 µg/mL [60] Tested at adult stage of fish…”

Section: Current Understanding Of Graphene and Graphene Oxide (Go) Tomentioning

confidence: 99%

Toxicity Studies on Graphene-Based Nanomaterials in Aquatic Organisms: Current Understanding

et al. 2020

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Graphene and its oxide are nanomaterials considered currently to be very promising because of their great potential applications in various industries. The exceptional physiochemical properties of graphene, particularly thermal conductivity, electron mobility, high surface area, and mechanical strength, promise development of novel or enhanced technologies in industries. The diverse applications of graphene and graphene oxide (GO) include energy storage, sensors, generators, light processing, electronics, and targeted drug delivery. However, the extensive use and exposure to graphene and GO might pose a great threat to living organisms and ultimately to human health. The toxicity data of graphene and GO is still insufficient to point out its side effects to different living organisms. Their accumulation in the aquatic environment might create complex problems in aquatic food chains and aquatic habitats leading to debilitating health effects in humans. The potential toxic effects of graphene and GO are not fully understood. However, they have been reported to cause agglomeration, long-term persistence, and toxic effects penetrating cell membrane and interacting with cellular components. In this review paper, we have primarily focused on the toxic effects of graphene and GO caused on aquatic invertebrates and fish (cell line and organisms). Here, we aim to point out the current understanding and knowledge gaps of graphene and GO toxicity.

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“…According to this study, GO at 100 mg/L concentration resulted in a longer hatching time for zebrafish embryos, shorter body length, changes in heart rate and blood flow, and an increase in apoptotic gene expression. When the concentration of GO exceeded 10 mg/L, the toxicity was not dependent on the size of the GO [166]. As a result, selecting the size and dosage of CNMs is critical for their safety and effectiveness in therapeutic applications.…”

Section: Carbon Nanomaterialsmentioning

confidence: 99%

Nanomaterials: A Promising Therapeutic Approach for Cardiovascular Diseases

Chopra

Bibi

Mishra

et al. 2022

Journal of Nanomaterials

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Cardiovascular diseases (CVDs) are a primary cause of death globally. A few classic and hybrid treatments exist to treat CVDs. However, they lack in both safety and effectiveness. Thus, innovative nanomaterials for disease diagnosis and treatment are urgently required. The tiny size of nanomaterials allows them to reach more areas of the heart and arteries, making them ideal for CVDs. Atherosclerosis causes arterial stenosis and reduced blood flow. The most common treatment is medication and surgery to stabilize the disease. Nanotechnologies are crucial in treating vascular disease. Nanomaterials may be able to deliver medications to lesion sites after being infused into the circulation. Newer point-of-care devices have also been considered together with nanomaterials. For example, this study will look at the use of nanomaterials in imaging, diagnosing, and treating CVDs.

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Toxic effects of different-sized graphene oxide particles on zebrafish embryonic development

Cited by 50 publications

References 45 publications

<p>Synthesis, Characterization, and Toxicity Assessment of Pluronic F127-Functionalized Graphene Oxide on the Embryonic Development of Zebrafish (<em>Danio rerio</em>)</p>

<p>Synthesis, Characterization, and Toxicity Assessment of Pluronic F127-Functionalized Graphene Oxide on the Embryonic Development of Zebrafish (<em>Danio rerio</em>)</p>

Toxicity Studies on Graphene-Based Nanomaterials in Aquatic Organisms: Current Understanding

Nanomaterials: A Promising Therapeutic Approach for Cardiovascular Diseases

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