Toxic effects of some pesticides on the enzymatic activities and spermatogenesis of the land snail Monacha cantiana

Abstract

Laboratory studies have been carried out on the toxic effects of three different pesticides namely, Diazinon, Lambdacyhalothrin and Methomyl as poisonous baits against the land snail, Monacha cantiana. The activities of five vital tissue enzymes, as Catalase (CAT), Malondialdehyde (MDA), Aspartate transaminase (AST). Alanine transaminase(ALT) and Alkaline phosphatase (ALP) were examined after exposure of these snails to LC 50 (5ppm) of the tested pesticides for 96hr. Results showed clearly that all sublethal concentrations of tested pesticides lead to a significant increase in the activities of the enzymatic levels within tissues of treated snails. On the other hand, LC 25 (2.5 ppm) of Diazinon was found to produce severe histopathological alterations in the hermaphrodite gland of M. cantiana, including, deterioration in germinal epithelium of the acini spermatocytes as well as reduction in numbers of sperms which are inhibited to develop to the late stages of maturity.

Oxidative and Histological Effects of Herbicide Glufosinate-Ammonium and Cyanobacteria Extracted Anatoxin-a on Land Snails Monacha cartusiana

“…The present study revealed that Anatoxin-a and Glufosinate-ammonium significantly increased ALP levels in the digestive gland of treated M. cartusiana. Such increments are in harmony with the results of Sharaf et al (2015) who stated that Diazinon, Lambda-cyhalothrin, and Methomyl exposure increased ALP levels in M. cartusiana. This increase may be due to destruction of the hepatic cell.…”

“…Such increment in CAT and GPX activities may be an adaptive mechanism to prevent the accumulation of toxic reactive oxygen (Regoli et al, 2006). In the same regard, Sharaf et al (2015) found that CAT activity increased in the digestive gland of the clover snail, M. cartusiana, after treatment with Diazinon, Lambda-cyhalothrin, and Methomyl. Glutathione-s-transferase (GST) plays a vital role in defending cells and tissues from oxidative stress (Havelková et al, 2008).…”

“…The current study displayed that MDA levels increased significantly in clover snail M. cartusiana after treatment with two pesticides. Likewise, Sharaf et al, (2015) illustrated that MDA levels increased in the clover snail, M. cartusiana, after exposure to Diazinon, Lambdacyhalothrin, and Methomyl. The increment may induce this elevation in free radicals, which causes the overproduction of MDA.…”

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“…The present study revealed that Anatoxin-a and Glufosinate-ammonium significantly increased ALP levels in the digestive gland of treated M. cartusiana. Such increments are in harmony with the results of Sharaf et al (2015) who stated that Diazinon, Lambda-cyhalothrin, and Methomyl exposure increased ALP levels in M. cartusiana. This increase may be due to destruction of the hepatic cell.…”

“…The current study displayed that MDA levels increased significantly in clover snail M. cartusiana after treatment with two pesticides. Likewise, Sharaf et al, (2015) illustrated that MDA levels increased in the clover snail, M. cartusiana, after exposure to Diazinon, Lambdacyhalothrin, and Methomyl. The increment may induce this elevation in free radicals, which causes the overproduction of MDA.…”