Toxic mechanism of pyrene to catalase and protective effects of vitamin C: Studies at the molecular and cell levels

Sun, Ning; Li, Meifei; Liu, Guiliang; Jing, Mingyang; He, Falin; Cao, Zhaozhen; Zong, Wansong; Tang, Jingchun; Gao, Canzhu; Liu, Rutao

doi:10.1016/j.ijbiomac.2020.12.169

Cited by 25 publications

(5 citation statements)

References 55 publications

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“…Soil catalase (S-CAT), a common antioxidant enzyme in soil ( Sun et al, 2021 ), can be used as an indicator of soil biomass to some extent, and soils with high biomass usually have higher catalase activity ( Chabot et al, 2020 ). The results in Figure 1 show that the highest S-CAT activity was observed in the LS treatment, indicating that the addition of sodium chloride reduced the S-CAT activity.…”

Section: Resultsmentioning

confidence: 99%

“…Soil enzymes are also common representations of soil biochemical characteristics, which are produced by soil microorganisms ( Cortés-Lorenzo et al, 2012 ; Singh, 2015 ; Azadi and Raiesi, 2021 ). Soil catalase (S-CAT) can decompose hydrogen peroxide in soil and reduce the damage of excessive accumulation of hydrogen peroxide to soil microorganisms ( Sun et al, 2021 ). Soil polyphenol oxidase (S-PPO) is an oxidoreductase that can oxidize aromatic compounds into quinones ( Sullivan, 2014 ).…”

Section: Introductionmentioning

confidence: 99%

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Effects of Salinity on the Biodegradation of Polycyclic Aromatic Hydrocarbons in Oilfield Soils Emphasizing Degradation Genes and Soil Enzymes

Yang

et al. 2022

Front. Microbiol.

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The biodegradation of organic pollutants is the main pathway for the natural dissipation and anthropogenic remediation of polycyclic aromatic hydrocarbons (PAHs) in the environment. However, in the saline soils, the PAH biodegradation could be influenced by soil salts through altering the structures of microbial communities and physiological metabolism of degradation bacteria. In the worldwide, soils from oilfields are commonly threated by both soil salinity and PAH contamination, while the influence mechanism of soil salinity on PAH biodegradation were still unclear, especially the shifts of degradation genes and soil enzyme activities. In order to explain the responses of soils and bacterial communities, analysis was conducted including soil properties, structures of bacterial community, PAH degradation genes and soil enzyme activities during a biodegradation process of PAHs in oilfield soils. The results showed that, though low soil salinity (1% NaCl, w/w) could slightly increase PAH degradation rate, the biodegradation in high salt condition (3% NaCl, w/w) were restrained significantly. The higher the soil salinity, the lower the bacterial community diversity, copy number of degradation gene and soil enzyme activity, which could be the reason for reductions of degradation rates in saline soils. Analysis of bacterial community structure showed that, the additions of NaCl increase the abundance of salt-tolerant and halophilic genera, especially in high salt treatments where the halophilic genera dominant, such as Acinetobacter and Halomonas. Picrust2 and redundancy analysis (RDA) both revealed suppression of PAH degradation genes by soil salts, which meant the decrease of degradation microbes and should be the primary cause of reduction of PAH removal. The soil enzyme activities could be indicators for microorganisms when they are facing adverse environmental conditions.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effects of Salinity on the Biodegradation of Polycyclic Aromatic Hydrocarbons in Oilfield Soils Emphasizing Degradation Genes and Soil Enzymes

Yang

et al. 2022

Front. Microbiol.

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“…We then screened seven typical DEPs associated with metabolic transformation and oxidative stress of DnBP and analyzed their interaction with DnBP using molecular docking. Changes in the protein structure due to ligand binding may affect their activity . We selected CYP93G2, glutathione transferase, NAD( P )H dehydrogenase, SOD, peroxidase, malate synthase, and heat shock protein Hsp20 in O.…”

Section: Results and Discussionmentioning

confidence: 99%

“…Changes in the protein structure due to ligand binding may affect their activity. 54 We selected CYP93G2, glutathione transferase, NAD(P)H dehydrogenase, SOD, peroxidase, malate synthase, and heat shock protein Hsp20 in O. sativa. Figure 2 shows the simulations of DnBP with CYP93G2, glutathione transferase, NAD(P)H dehydrogenase, and SOD in rice.…”

Section: Protein Molecular Response Mechanism By Molecular Docking Si...mentioning

confidence: 99%

Interaction between Phthalate Ester and Rice Plants: Novel Transformation Pathways and Metabolic-Network Perturbations

Xing

Sun

et al. 2023

Environ. Sci. Technol.

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Our understanding is limited concerning the interaction mechanism between widespread phthalate esters and staple crops, which have strong implications for human exposure. Therefore, this study was aimed at illuminating the transformation pathways of di-n-butyl phthalate (DnBP) in rice using an untargeted screening method. UPLC-QTOF-MS identified 16 intermediate transformation products formed through hydroxylation, hydrolysis, and oxidation in phase I metabolism and further by conjugation with amino acids, glutathione, and carbohydrates in phase II metabolism. Mono-2-hydroxy-n-butyl phthalate-L-aspartic acid (MHBP-asp) and mono-2-hydroxy-n-butyl phthalate-D-alanylβ-D-glucoside (MHBP-ala-glu) products were observed for the first time. The proteomic analysis demonstrated that DnBP upregulated the expression of rice proteins associated with transporter activity, antioxidant synthesis, and oxidative stress response and downregulated that of proteins involved in photosynthesis, photorespiration, chlorophyll binding, and mono-oxygenase activity. Molecular docking revealed that DnBP can affect protein molecular activity via pi−sigma, pi−alkyl, and pi−pi interactions or by forming carbon−hydrogen bonds. The metabolomic analysis showed that key metabolic pathways including citrate cycle, biosynthesis of aminoacyl-tRNA, and metabolism of amino acids, sphingolipids, carbohydrates, nucleotides, and glutathione were activated in rice plants exposed to DnBP and its primary metabolite mono-n-butyl phthalate (MnBP). Furthermore, exposure to 80 ng/mL MnBP significantly perturbed the metabolic profile and molecular function in plants, with downregulation of the levels of beta-alanine (0.56-fold), cytosine (0.48-fold), thymine (0.62-fold), uracil (0.48-fold), glucose (0.59-fold), and glucose-1-phosphate (0.33-fold), as well as upregulation of the levels of L-glutamic acid (2.97-fold), L-cystine (2.69-fold), and phytosphingosine (38.38-fold). Therefore, the degradation intermediates of DnBP pose a potentially risk to plant metabolism and raise concerns for crop safety related to plasticizer pollution.

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“…The antioxidant response was known as a primary defence for those organisms under stress to protect cells and macromolecules from oxidative damage (Hu et al, 2016), whereas enough energy and material were essential J o u r n a l P r e -p r o o f like minerals and vitamins. For example, Sun et al (2021) validated the extra addition of vitamin C alleviated the antioxidant and protected earthworm E.fetida from the toxic damage of polycyclic aromatic hydrocarbons.…”

Section: Transcriptional Mechanisms Behind the Earthworm Agnp Toxicit...mentioning

confidence: 98%

Impact of progressively cumulative exposure of AgNPs on earthworms (Eisenia fetida) and implication for eco-toxicological risk assessment

et al. 2023

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Toxic mechanism of pyrene to catalase and protective effects of vitamin C: Studies at the molecular and cell levels

Cited by 25 publications

References 55 publications

Effects of Salinity on the Biodegradation of Polycyclic Aromatic Hydrocarbons in Oilfield Soils Emphasizing Degradation Genes and Soil Enzymes

Effects of Salinity on the Biodegradation of Polycyclic Aromatic Hydrocarbons in Oilfield Soils Emphasizing Degradation Genes and Soil Enzymes

Interaction between Phthalate Ester and Rice Plants: Novel Transformation Pathways and Metabolic-Network Perturbations

Impact of progressively cumulative exposure of AgNPs on earthworms (Eisenia fetida) and implication for eco-toxicological risk assessment

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