Toxicity assessment of <i>Lavandula officinalis</i> extracts in Brine Shrimp (<i>Artemia salina</i>)

Braguini, Welligton Luciano; Alves, Bruno Bianchin; Pires, Natália Valendolf

doi:10.1080/15376516.2019.1567892

Cited by 13 publications

(8 citation statements)

References 45 publications

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“…A recent study has already demonstrated that it is also possible to evaluate and quantify these compounds with antioxidant activities through a biosensing approach [ 36 ]. Lavender extract, recognized for its cytotoxicity, demonstrated the presence of tannins and saponins [ 37 ], a finding consistent with our present study, along with the discovery of flavonoids. Notably, wormwood and sage extracts exhibited saponins exclusively, which have been harnessed within liposomes to activate a cancer response system [ 38 ].…”

Section: Discussionsupporting

confidence: 92%

Rapid and Versatile Biosensing of Liposome Encapsulation Efficiency Using Electrical Conductivity Sensor

Pereira,

Bonatto,

Silva

2023

Biosensors

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Liposomes are prominent nanosystems for drug delivery, with potential extending beyond isolated drugs. Ethanol-aqueous plant extracts can be encapsulated within liposomes to protect bioactive compounds (secondary metabolites) from rapid oxidation and enable sustained release. Determining which compound classes are present in each extract and the encapsulation efficiency (EE) of these extracts in liposomes is crucial for nanocarrier functionality. This involves assessing the ratio of bioactive substances within liposomes to the total content. However, quantifying EE for non-isolated compounds poses challenges due to the need for advanced analytical equipment and biosensing approaches. This study introduces an innovative method for EE quantification, using a conductivity electrode (k = 0.842/cm) to establish an EE biosensing technology. By correlating dynamic light scattering (DLS), zeta potential (ZP), and electrical conductivity (Cnd) data with the conductivity meter’s calibration curve, a robust relationship between the free extract concentration and Cnd (r2 ≥ 0.950) was established. Lavender-loaded liposomes demonstrated an EE of 56.33%, while wormwood and oregano formulations exhibited high EEs of 94.33% and 91.70%, respectively. In contrast, sage-loaded liposomes exhibited an inadequate EE, encapsulating only approximately 0.57% of the extract. The straightforward quantification of the free extract within liposome formulations, compared to more complex approaches, could facilitate EE determination and support future characterizations.

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Section: Discussionsupporting

confidence: 92%

Rapid and Versatile Biosensing of Liposome Encapsulation Efficiency Using Electrical Conductivity Sensor

Pereira,

Bonatto,

Silva

2023

Biosensors

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“…The values are shown in Table 2 . Values of LC50 < 80 µg·mL −1 are considered highly toxic [ 15 , 61 , 62 ]; values of LC 50 within the range 80 to 250 µg·mL −1 are moderately toxic; and LC 50 > 250 µg·mL −1 are considered as low toxicity or non-toxic [ 63 ]. The EOs of D. echinophora , X. emarginata and X. frutescens showed high toxicity, whereas the EO of D. riparia showed low toxicity or was non-toxic.…”

Section: Resultsmentioning

confidence: 99%

Phytochemical Profile, Antioxidant Potential and Toxicity Evaluation of the Essential Oils from Duguetia and Xylopia Species (Annonaceae) from the Brazilian Amazon

et al. 2022

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The essential oils (EOs) of Duguetia echinophora, D. riparia, Xylopia emarginata and X. frutescens (Annonaceae) were obtained by hydrodistillation and the chemical composition was analyzed by GC-MS. An antioxidant assay using the ABTS and DPPH radicals scavenging method and cytotoxic assays against Artemia salina were also performed. We evaluated the interaction of the major compounds of the most toxic EO (X. emarginata) with the binding pocket of the enzyme Acetylcholinesterase, a molecular target related to toxicity in models of Artemia salina. The chemical composition of the EO of D. echinophora was characterized by β-phellandrene (39.12%), sabinene (17.08%) and terpinolene (11.17%). Spathulenol (22.22%), caryophyllene oxide (12.21%), humulene epoxide II (11.86%) and allo-aromadendrene epoxide (10.20%) were the major constituents of the EO from D. riparia. Spathulenol (5.65%) and caryophyllene oxide (5.63%) were the major compounds of the EO from X. emarginata. The EO of X. frutescens was characterized by α-pinene (20.84%) and byciclogermacrene (7.85%). The results of the radical scavenger DPPH assays ranged from 15.87 to 69.38% and the highest percentage of inhibition was observed for the EO of X. emarginata, while for ABTS radical scavenging, the antioxidant capacity of EOs varied from 14.61 to 63.67%, and the highest percentage of inhibition was observed for the EO of X. frutescens. The EOs obtained from D. echinophora, X. emarginata and X. frutescens showed high toxicity, while the EO of D. riparia was non-toxic. Because the EO of X. emarginata is the most toxic, we evaluated how its major constituents were able to interact with the Acetylcholinesterase enzyme. The docking results show that the compounds are able to bind to the binding pocket through non-covalent interactions with the residues of the binding pocket. The species X. emarginata and X. frutescens are the most promising sources of antioxidant compounds; in addition, the results obtained for preliminary cytotoxicity of the EOs of these species may also indicate a potential biological activity.

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“…Some examples of other essential oil samples, with different plant origins and different biological functions, are: Citrus limon (LC 50 =743.35 mg L −1 ), [32] Hyptis suaveolens (LC 50 =49.72 μg mL −1 ), [33] and Syzygium aromaticum (LC 50 =0.59±0.04 μg mL −1 ) [34] . Low LC 50 values in Artemia salina demonstrate that the extract is highly toxic and harmful to humans, and should therefore be administered with care to avoid poisoning [33,35] …”

Section: Resultsmentioning

confidence: 99%

Extraction Yield, Chemical Composition, Preliminary Toxicity of Bignonia nocturna (Bignoniaceae) Essential Oil and in Silico Evaluation of the Interaction

Oliveira

Silva

Freitas

et al. 2021

Chemistry & Biodiversity

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Bignonia nocturna (Bignoniaceae) is a plant used for medicinal purposes by the Amazonian indigenous peoples. To date, there have been no reported studies on its toxicity. The present study aimed to evaluate the chemical composition of essential oils obtained from Bignonia nocturna by different extraction techniques. In addition, an in silico study of the molecular interactions was performed using molecular docking and molecular dynamics. The extractions were carried out by hydrodistillation, simultaneous distillation‐extraction, and steam distillation, using samples collected from the Amazon in summer and winter. The chemical composition was analyzed by GC/FID and GC/MS, and the cytotoxic activity in Artemia salina Leach was evaluated. The maximum yield (1.38 % w/w) was obtained by hydrodistillation. The results indicated that benzaldehyde predominated in all the fractions of both the volatile concentrate and the essential oils. In addition, the oil proved to be highly toxic to Artemia salina. The computer simulation results indicated that benzaldehyde strongly interacts with acetylcholinesterase, which is the likely interaction mechanism responsible for the cytotoxicity.

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Toxicity assessment of Lavandula officinalis extracts in Brine Shrimp (Artemia salina)

Cited by 13 publications

References 45 publications

Rapid and Versatile Biosensing of Liposome Encapsulation Efficiency Using Electrical Conductivity Sensor

Rapid and Versatile Biosensing of Liposome Encapsulation Efficiency Using Electrical Conductivity Sensor

Phytochemical Profile, Antioxidant Potential and Toxicity Evaluation of the Essential Oils from Duguetia and Xylopia Species (Annonaceae) from the Brazilian Amazon

Extraction Yield, Chemical Composition, Preliminary Toxicity of Bignonia nocturna (Bignoniaceae) Essential Oil and in Silico Evaluation of the Interaction

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