Toxicity evaluation of some traditional African spices on breast cancer cells and isolated rat hepatic mitochondria

Choumessi, Aphrodite T.; Loureiro, Rute; Silva, Ana Maria; Moreira, Ana C.; Pieme, Anatole Constant; Asonganyi, Tazoacha; Oliveira, Paulo J.; Penlap, Véronique

doi:10.1016/j.fct.2012.08.008

Cited by 22 publications

(13 citation statements)

References 36 publications

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“…In Cameroon, the dried fruits are traditionally used as a spice in soups [8]. Literature has also shown that extracts of this plant possess a potential antimicrobial [9][10][11][12][13], insecticidal [14], antiplasmodial [15], cytotoxic [16,17], anti-inflammatory [18] and antioxidant activities [18][19][20].…”

Section: Introductionmentioning

confidence: 99%

The Flavonoid Compounds from Zanthoxylum leprieurii Guill. et Perr (Rutaceae) Extracts and their Antioxidant Activity against ABTS•+

Tine

Diallo

Ndoye

et al. 2020

J. Drug Delivery Ther.

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Screening of flavonoid compounds in fruit, leaf, stem and root bark extracts of Z. leprieurii was achieved by liquid chromatography (LC) coupled with tandem mass spectrometry (MS2). Among the 186 reference compounds tested, four flavonoids were identified in one or other of the organ extracts from each plant (fruit, leaf, root barks and stem): neodiosmin and hesperidin were identified in all extracts. The presence of datiscin was reported only in leaves while that of rutin was identified in fruits and leaves. Of these four flavonoids, three (neodiosmin, datiscin and rutin) are detected for the first time in Z. leprieurii. The antioxidant properties of different extracts were tested regarding their scavenging activities on ABTS•+ radical. Fruit, leaf and stem extracts had low antioxidant potential and root bark extracts exhibited very low antioxidant activity. Keywords: Zanthoxylum leprieurii, flavonoids, LC-MS/MS and antioxidant activity.

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Section: Introductionmentioning

confidence: 99%

The Flavonoid Compounds from Zanthoxylum leprieurii Guill. et Perr (Rutaceae) Extracts and their Antioxidant Activity against ABTS•+

Tine

Diallo

Ndoye

et al. 2020

J. Drug Delivery Ther.

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“…Besides the studies on its essential oil, no work has been carried out on the constituents of its non volatile extract. A recent study has revealed that the alcoholic extract of the fruits of this plant possessed in vitro cytotoxic activity against MCF-7, without identifying any bioactive constituents [17]. This prompted us to undertake a comparative study on the chemical constituents of the fruits of Z. zanthoxyloides and Z. leprieurii to identify their bioactive compounds.…”

Section: Introductionmentioning

confidence: 99%

Aromatase and glycosyl transferase inhibiting acridone alkaloids from fruits of Cameroonian Zanthoxylumspecies

Wouatsa

Misra

Kumar

et al. 2013

Chemistry Central Journal

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BackgroundZanthoxylum zanthoxyloides and Z. leprieurii fruits are commonly used in traditional system of medicine for diarrhea, pain, wound healing, etc. in Cameroon, Africa. Z. leprieurii fruits have been chemically studied for its bioactive compounds whereas the investigation on Z. zanthoxyloides fruits is lacking.ResultsAfter a detailed chemical analysis of the fruits of Z. leprieurii and Z. zanthoxyloides, a series of new acridone alkaloids, namely, 3-hydroxy-1,5,6-trimethoxy-9-acridone (1), 1,6-dihydroxy-3-methoxy-9-acridone (2), 3,4,5,7-tetrahydroxy-1-methoxy-10-methyl-9-acridone (3), 4-methoxyzanthacridone (8), 4-hydroxyzanthacridone (9), 4-hydroxyzanthacridone oxide (2,4’) (10) have been isolated. The known acridones which have been characterized are, helebelicine A (4), 1-hydroxy-3-methoxy-10-methyl-9-acridone (5), 1,3-dihydroxy-4-methoxy-10-methyl-9-acridone (6) and tegerrardin A (7). The in vitro antibacterial and cytotoxic screening of these acridones reveal that compound 3 has a moderate antibacterial activity (MIC 125 μg/mL) against Micrococcus luteus and Pseudomonas aeruginosa while compound 1 shows a moderate cytotoxic effect (IC50 of 86 μM) against WRL-68 (liver cancer cell line). Furthermore, the molecular modeling of these acridones predicted the structural basis for their mode of action and binding affinity for aromatase, quinone reductase and WAAG, a glycosyltransferase involved in bacterial lipopolysaccharide synthesis. Computational approaches, quantitative SAR and modeling studies predicted that acridones 1, 2, 3, 4, 9 and 10 were the inhibitors of glycosyltransferase while 1, 8, 9 and 10, the inhibitors of aromatase.ConclusionsA total of 10 acridones have been isolated out of which 6 are new (1, 2, 3, 8, 9 and 10). Alkaloids 8, 9 and 10, having novel tetracyclic acridone structure with new carbon skeleton, have now been named as zanthacridone. The quantitative SAR and molecular modeling studies suggested that the compounds 1, 9 and 10 are inhibitors of both aromatase and glycosyltransferase.

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“…For instance, ursolic acid, a bioactive component in some herbs and spices, suppressed TNF-induced expression of genes regulated by NF-κB (cyclin D1, COX-2, and MMP-9) which are involved in tumor initiation, promotion, and metastasis [7]. In Cameroon, several studies have been carried on the cytotoxic activity of some spices on different cell lines [8-10]. …”

Section: Introductionmentioning

confidence: 99%

Induction of mitochondrial dependent apoptosis and cell cycle arrest in human promyelocytic leukemia HL-60 cells by an extract from Dorstenia psilurus: a spice from Cameroon

Pieme

Guru

Ambassa

et al. 2013

BMC Complement Altern Med

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BackgroundThe use of edible plants is an integral part of dietary behavior in the West region of Cameroon. Dorstenia psilurus (Moraceae) is widely used as spice and as medicinal plant for the treatment of several diseases in Cameroon. The aim of this study is to investigate the cytotoxic and apoptotic potential of methanol extract of D. psilurus in human promyelocytic leukemia (HL-60) cells and prostate cancer (PC-3) cells.MethodsCytotoxicity of D. psilurus extract was tested in HL-60 and PC-3 cells using 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction assay and flow cytometric methodsResultsThe methanol extract of D. psilurus have significant in vitro cytotoxic activity in HL-60 cells and PC-3 cells with IC50 value of 12 ±1.54 μg/ml and 18 ± 0.45 μg/ml respectively after 48 h. The mechanism of antiproliferative activity showed that after 24 h, D. psilurus extract induces apoptosis on HL-60 cells by the generation of reactive oxygen species (ROS) along with concurrent loss of mitochondrial membrane potential, modification in the DNA distribution and enhance of G2/M phase cell cycle.ConclusionThe extract induces apoptosis of HL-60 cells associated with ROS production, loss of mitochondrial membrane potential and apoptotic DNA fragmentation.

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Toxicity evaluation of some traditional African spices on breast cancer cells and isolated rat hepatic mitochondria

Cited by 22 publications

References 36 publications

The Flavonoid Compounds from Zanthoxylum leprieurii Guill. et Perr (Rutaceae) Extracts and their Antioxidant Activity against ABTS•+

The Flavonoid Compounds from Zanthoxylum leprieurii Guill. et Perr (Rutaceae) Extracts and their Antioxidant Activity against ABTS•+

Aromatase and glycosyl transferase inhibiting acridone alkaloids from fruits of Cameroonian Zanthoxylumspecies

Induction of mitochondrial dependent apoptosis and cell cycle arrest in human promyelocytic leukemia HL-60 cells by an extract from Dorstenia psilurus: a spice from Cameroon

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