Toxicity of Carbon, Silicon, and Metal-Based Nanoparticles to Sea Urchin Strongylocentrotus intermedius

Pikula, Konstantin; Zakharenko, Alexander M.; Чайка, Владимир; Em, Iurii M.; Nikitina, A. V.; Автомонов, Е. Г.; Tregubenko, Anna; Agoshkov, A. I.; Mishakov, Ilya V.; Кузнецов, В. Л.; Gusev, Alexander; Park, Soo‐Jin; Golokhvast, Kirill S.

doi:10.3390/nano10091825

Cited by 28 publications

(18 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The embryos that reached correct development in 24 h were defined as normal. The features of normal development were described previously [34]. The number of normal embryos (Figure S2c), the embryos with developmental delays or impaired development (Figure S2d), and dead embryos were counted relative to the total number of zygotes.…”

Section: Sea Urchin Bioassaymentioning

confidence: 93%

“…The protocol of the sea urchin bioassay has been described previously [34]. Adult individuals of the sea urchin S. intermedius were collected from Novi Dzhigit Bay (Peter the Great Bay, Sea of Japan, Russia).…”

Section: Sea Urchin Bioassaymentioning

confidence: 99%

“…Moreover, it can demonstrate the potential of pollution to transfer through aquatic food chains. The embryos of sea urchins are a sensitive and reliable indicator of marine pollutions [34,35]. Sea urchin sperm and embryo-larval bioassays are efficiently applied in ecotoxicity bioassays due to their sensitivity to very low concentrations of pollutants in seawater [36].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Environmental Risk Assessment of Vehicle Exhaust Particles on Aquatic Organisms of Different Trophic Levels

Pikula

Tretyakova

Zakharenko

et al. 2021

Toxics

Self Cite

View full text Add to dashboard Cite

Vehicle emission particles (VEPs) represent a significant part of air pollution in urban areas. However, the toxicity of this category of particles in different aquatic organisms is still unexplored. This work aimed to extend the understanding of the toxicity of the vehicle exhaust particles in two species of marine diatomic microalgae, the planktonic crustacean Artemia salina, and the sea urchin Strongylocentrotus intermedius. These aquatic species were applied for the first time in the risk assessment of VEPs. Our results demonstrated that the samples obtained from diesel-powered vehicles completely prevented egg fertilization of the sea urchin S. intermedius and caused pronounced membrane depolarization in the cells of both tested microalgae species at concentrations between 10 and 100 mg/L. The sample with the highest proportion of submicron particles and the highest content of polycyclic aromatic hydrocarbons (PAHs) had the highest growth rate inhibition in both microalgae species and caused high toxicity to the crustacean. The toxicity level of the other samples varied among the species. We can conclude that metal content and the difference in the concentrations of PAHs by itself did not directly reflect the toxic level of VEPs, but the combination of both a high number of submicron particles and high PAH concentrations had the highest toxic effect on all the tested species.

show abstract

Section: Sea Urchin Bioassaymentioning

confidence: 93%

Section: Sea Urchin Bioassaymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Environmental Risk Assessment of Vehicle Exhaust Particles on Aquatic Organisms of Different Trophic Levels

Pikula

Tretyakova

Zakharenko

et al. 2021

Toxics

Self Cite

View full text Add to dashboard Cite

show abstract

“…In another study, Konstantin Pikula and co-workers demonstrated the use of sea urchin Strongylocentrotus intermedius as a novel approach to assess the nanotoxicity of NMs, such as Au NPs, silicon nanotubes (Si NTs), TiO 2 NPs, cadmium, and zinc sulfide (CdS and ZnS) nanocrystals. In summary, they observed the following trend in toxicity: Au NPs > Si NTs > CdS > ZnS > TiO 2 [94].…”

Section: Metallic-based Nanoparticlesmentioning

confidence: 93%

A Review on Conventional and Advanced Methods for Nanotoxicology Evaluation of Engineered Nanomaterials

et al. 2021

View full text Add to dashboard Cite

Nanotechnology can be defined as the field of science and technology that studies material at nanoscale (1–100 nm). These nanomaterials, especially carbon nanostructure-based composites and biopolymer-based nanocomposites, exhibit excellent chemical, physical, mechanical, electrical, and many other properties beneficial for their application in many consumer products (e.g., industrial, food, pharmaceutical, and medical). The current literature reports that the increased exposure of humans to nanomaterials could toxicologically affect their environment. Hence, this paper aims to present a review on the possible nanotoxicology assays that can be used to evaluate the toxicity of engineered nanomaterials. The different ways humans are exposed to nanomaterials are discussed, and the recent toxicity evaluation approaches of these nanomaterials are critically assessed.

show abstract

“…Our previous works addressed the question based on the responsible use of animals in biomedical research (the 3R principle) [ 13 ], focusing on a new methodology for in vitro/ex vivo nanostudies based on a nonmammalian model that is a proxy for humans, the sea urchin (marine invertebrate) [ 14 ]. Several studies have displayed the suitability of the sea urchin as a model for risk assessment in environmental nanotoxicology [ 15 , 16 ]. Our recent report on sea urchin immune cells exposed to AuNPs functionalized with polyvinylpyrrolidone (PVP–AuNPs, 1 and 10 μg mL −1 ) underlined the capability of particles to activate a transient innate immune response independently of the concentration used [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

Immunomodulatory Function of Polyvinylpyrrolidone (PVP)-Functionalized Gold Nanoparticles in Vibrio-Stimulated Sea Urchin Immune Cells

et al. 2021

View full text Add to dashboard Cite

We investigated the role of the gold nanoparticles functionalized with polyvinylpyrrolidone (PVP–AuNPs) on the innate immune response against an acute infection caused by Vibrio anguillarum in an in vitro immunological nonmammalian next-generation model, the sea urchin Paracentrotus lividus. To profile the immunomodulatory function of PVP–AuNPs (0.1 μg mL−1) in sea urchin immune cells stimulated by Vibrio (10 μg mL−1) for 3 h, we focused on the baseline immunological state of the donor, and we analysed the topography, cellular metabolism, and expression of human cell surface antigens of the exposed cells, as well as the signalling leading the interaction between PVP–AuNPs and the Vibrio-stimulated cells. PVP–AuNPs are not able to silence the inflammatory signalling (TLR4/p38MAPK/NF-κB signalling) that involves the whole population of P. lividus immune cells exposed to Vibrio. However, our findings emphasise the ability of PVP–AuNPs to stimulate a subset of rare cells (defined here as Group 3) that express CD45 and CD14 antigens on their surface, which are known to be involved in immune cell maturation and macrophage activation in humans. Our evidence on how PVP–AuNPs may stimulate sea urchin immune cells represents an important starting point for planning new research work on the topic.

show abstract

Toxicity of Carbon, Silicon, and Metal-Based Nanoparticles to Sea Urchin Strongylocentrotus intermedius

Cited by 28 publications

References 59 publications

Environmental Risk Assessment of Vehicle Exhaust Particles on Aquatic Organisms of Different Trophic Levels

Environmental Risk Assessment of Vehicle Exhaust Particles on Aquatic Organisms of Different Trophic Levels

A Review on Conventional and Advanced Methods for Nanotoxicology Evaluation of Engineered Nanomaterials

Immunomodulatory Function of Polyvinylpyrrolidone (PVP)-Functionalized Gold Nanoparticles in Vibrio-Stimulated Sea Urchin Immune Cells

Contact Info

Product

Resources

About