Toxicity of Fe<sub>2</sub>O<sub>3</sub> nanoparticles on human blood lymphocytes

Assadian, Evelyn; Dezhampanah, Hamid; Seydi, Enayatollah; Pourahmad, Jalal

doi:10.1002/jbt.22303

Cited by 20 publications

(11 citation statements)

References 30 publications

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“…Finally, a fluorescence spectrophotometer (Shimadzu RF5000U) was used at EXλ = 470 nm and EMλ = 540 nm to measure the fluorescence intensity. [ 29 ]…”

Section: Methodsmentioning

confidence: 99%

Selective anticancer activity of superparamagnetic iron oxide nanoparticles (SPIONs) against oral tongue cancer using in vitro methods: The key role of oxidative stress on cancerous mitochondria

Jahanbani

Ghotbi

Shahsavari

et al. 2020

J Biochem & Molecular Tox

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Today, it has been proven that the nanoparticles such as superparamagnetic iron oxide nanoparticles (SPIONs) have widespread use in biomedical applications, for instance, in magnetic resonance imaging and targeted delivery of drugs. Despite many studies on SPIONs in diagnosing some diseases like cancer, it has not been investigated on the oral tongue squamous cell carcinoma (OTSCC) detection by the NPs. Hence, the present study has been designed to assess the in vitro cytotoxicity of SPIONs on the isolated mitochondria of OTSCC by mitochondrial tests. Isolated mitochondria were removed from the separated cancer and control tissues from the squamous cells of tango in male Wistar rats (6 or 8 weeks) and exposed to the different concentrations of SPIONs (30, 60, and 120 nM). A rise in the production of reactive oxygen species is one of the significant mechanisms of this study, followed by a collapse of mitochondrial membrane potential, the escape of mitochondrial cytochrome c, and mitochondrial swelling in the exposed isolated mitochondria of OTSCC with SPIONs. Furthermore, our results indicated that the exposure to the SPIONs reduced the activity of succinate dehydrogenase in complex II of the mitochondria obtained from cancerous oral tongue squamous. So the SPIONs can induce selective cytotoxicity on the OTSCC mitochondria without significant effects on the control mitochondria. Based on the results and further studies about in vivo experiments in this regard, it is concluded the SPIONs may be a hopeful therapeutic candidate for the treatment of OTSCC. K E Y W O R D S cytotoxicity, mitochondria, oral tongue squamous cell carcinoma, oxidative stress, superparamagnetic iron oxide nanoparticles 1 | INTRODUCTION Oral tongue squamous cell carcinoma (OTSCC) is the most common malignancy of the oral cavity. The incidence of OTSCC is increasing, [1,2] and it has an aggressive clinical behavior with a relatively poor prognosis. [3] The most important risk factors for the development of OTSCC and tongue and oral cavity cancer are tobacco and alcohol; however, tobacco is more carcinogenic than alcohol. [4-7] It has been Jahanfar Jahanbani and Maryam Ghotbi equally share the position of 1st author in this article.

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“…Finally, a fluorescence spectrophotometer (Shimadzu RF5000U) was used at EXλ = 470 nm and EMλ = 540 nm to measure the fluorescence intensity. [ 29 ]…”

Section: Methodsmentioning

confidence: 99%

Selective anticancer activity of superparamagnetic iron oxide nanoparticles (SPIONs) against oral tongue cancer using in vitro methods: The key role of oxidative stress on cancerous mitochondria

Jahanbani

Ghotbi

Shahsavari

et al. 2020

J Biochem & Molecular Tox

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“…The mechanisms of the toxicity of these NPs should be determined. The oxidative stress (OS) induced by NPs has become one of the toxic mechanisms of NPs [20,21].…”

Section: Introductionmentioning

confidence: 99%

Protective Effect of Lactobacillus rhamnosus GG on TiO2 Nanoparticles-Induced Oxidative Stress Damage in the Liver of Young Rats

et al. 2021

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The potential toxicity of titanium dioxide nanoparticles (TiO2 NPs) to mammals has become a widespread concern. Young individuals exposed to TiO2 NPs have a higher risk than adults. In this study, the protective effects of Lactobacillus rhamnosus GG (LGG) on liver toxicity in young rats induced by TiO2 NPs were explored. Results show that the four-week-old rats that underwent LGG after the oral intake of TiO2 NPs could prevent weight loss, reduce hematological indicators (WBC and NEUT) and serum biochemical indicators (AST, ALT, AST/ALT, and ALP). Moreover, it alleviated the pathological damage of the liver (as indicated by the disordered hepatocytes, more eosinophilic, ballooning degeneration, and accompany with blood cells), but it did not reduce the Ti contents in the liver. In addition, RT-qPCR results indicated that LGG restored the expression of anti-oxidative stress-related genes, such as SOD1, SOD2, CAT, HO-1, GSH, GCLC, and GCLM in the liver. In summary, the hepatotoxicity of TiO2 NPs in young rats is closely related to oxidative stress, and the antioxidant effect of LGG might protect the harmful effects caused by TiO2 NPs.

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“…Evidently, the possible toxicity study of Fe 2 O 3 in human cells, including blood lymphocytes indicated that Fe 2 O 3 significantly reduced the cell viability and at different concentrations, Fe 2 O 3 NPs affect cellular glutathione (GSH) level and cause oxidative stress in human lymphocytes. [18]…”

Section: Synthesis and Characterizationmentioning

confidence: 99%

Synthesis, characterization and evaluation of novel ferrocenylmethylamine derivatives as cytotoxic agents

Savani

Roy

Verma

et al. 2021

Applied Organom Chemis

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The present report describes a new series of amide functionalized 2 0-and 3 0-aminomethylferrocene derived from ferrocenylmethylamine. The compounds 1a-5a and 1b-5b were characterized by microanalysis, 1 H, 13 C NMR, UV-visible, fluorescence, FTIR, thermogravimetric and crystallographic techniques. The X-ray analysis demonstrated the ability of these molecules to form various intermolecular hydrogen bonding interactions, as verified by Hirshfeld surface analysis. All the compounds were evaluated against MCF 7, IMR 32, HepG2 and immortal L132 cell lines by MTT assay and the results were compared with cisplatin. Interestingly, many compounds were very active against all the investigated cell lines and proved to be more potent as cytotoxic agents than cisplatin. The western blot, gene expression, mitochondrial membrane potential and flow cytometry study were used to investigate the mode of action of these derivatives as antitumor agents. The results showed apoptotic property of the compounds by modulating inflammatory pathway against human tumor cells of different origin. We performed the density functional theory calculations and molecular docking to rationalize the experimental results. K E Y W O R D S amide functionalized 2 0-and 3 0-aminomethylferrocene, anticancer, apoptosis, molecular docking, X-ray diffraction

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Toxicity of Fe₂O₃ nanoparticles on human blood lymphocytes

Cited by 20 publications

References 30 publications

Selective anticancer activity of superparamagnetic iron oxide nanoparticles (SPIONs) against oral tongue cancer using in vitro methods: The key role of oxidative stress on cancerous mitochondria

Selective anticancer activity of superparamagnetic iron oxide nanoparticles (SPIONs) against oral tongue cancer using in vitro methods: The key role of oxidative stress on cancerous mitochondria

Protective Effect of Lactobacillus rhamnosus GG on TiO2 Nanoparticles-Induced Oxidative Stress Damage in the Liver of Young Rats

Synthesis, characterization and evaluation of novel ferrocenylmethylamine derivatives as cytotoxic agents

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Toxicity of Fe2O3 nanoparticles on human blood lymphocytes

Cited by 20 publications

References 30 publications

Selective anticancer activity of superparamagnetic iron oxide nanoparticles (SPIONs) against oral tongue cancer using in vitro methods: The key role of oxidative stress on cancerous mitochondria

Selective anticancer activity of superparamagnetic iron oxide nanoparticles (SPIONs) against oral tongue cancer using in vitro methods: The key role of oxidative stress on cancerous mitochondria

Protective Effect of Lactobacillus rhamnosus GG on TiO2 Nanoparticles-Induced Oxidative Stress Damage in the Liver of Young Rats

Synthesis, characterization and evaluation of novel ferrocenylmethylamine derivatives as cytotoxic agents

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Resources

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Toxicity of Fe₂O₃ nanoparticles on human blood lymphocytes