Toxicity of fluralaner against vegetable pests and its sublethal impact on a biocontrol predatory ladybeetle

Liu, Kebin; Khan, Muhammad Musa; Fajar, Anugerah; Chen, Shimin; Guo, Mujuan; Chen, Yueyin; Yang, Chunxiao; Wu, Jing; Qiu, Bao‐Li; Zhou, Xuguo; Pan, Huipeng

doi:10.1016/j.ecoenv.2021.112743

Cited by 14 publications

(12 citation statements)

References 41 publications

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“…Synthetic insecticides are a relatively reliable, effective and rapid tool for managing pest problems ( Khan et al, 2018 , 2020 ; 2021b ). However, there are numerous problems associated with unmonitored and unplanned insecticide applications (e.g., adverse effects on the environment and harmful effects on nontarget organisms, such as humans, land and aquatic animals, and other arthropods), and the most important problem is the development of resistance ( Nawaz et al, 2017 ; Rasheed et al, 2020 ; Liu et al, 2021 ). Increased insecticide resistance imposes challenges on the sustainable management of many insect infestations ( Bass and Jones, 2018 ).…”

Section: Discussionmentioning

confidence: 99%

Transcriptome analysis reveals differential effects of beta-cypermethrin and fipronil insecticides on detoxification mechanisms in Solenopsis invicta

Siddiqui

Luo

Sheikh³

et al. 2022

Front. Physiol.

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Insecticide resistance poses many challenges in insect pest control, particularly in the control of destructive pests such as red imported fire ants (Solenopsis invicta). In recent years, beta-cypermethrin and fipronil have been extensively used to manage invasive ants, but their effects on resistance development in S. invicta are still unknown. To investigate resistance development, S. invicta was collected from populations in five different cities in Guangdong, China. The results showed 105.71- and 2.98-fold higher resistance against fipronil and beta-cypermethrin, respectively, in the Guangzhou population. The enzymatic activities of acetylcholinesterase, carboxylases, and glutathione S-transferases significantly increased with increasing beta-cypermethrin and fipronil concentrations. Transcriptomic analysis revealed 117 differentially expressed genes (DEGs) in the BC-ck vs. BC-30 treatments (39 upregulated and 78 downregulated), 109 DEGs in F-ck vs. F-30 (33 upregulated and 76 downregulated), and 499 DEGs in BC-30 vs. F-30 (312 upregulated and 187 downregulated). Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that DEGs associated with insecticide resistance were significantly enriched in metabolic pathways, the AMPK signaling pathway, the insulin signaling pathway, carbon metabolism, peroxisomes, fatty acid metabolism, drug metabolism enzymes and the metabolism of xenobiotics by cytochrome P450. Furthermore, we found that DEGs important for insecticide detoxification pathways were differentially regulated under both insecticide treatments in S. invicta. Comprehensive transcriptomic data confirmed that detoxification enzymes play a significant role in insecticide detoxification and resistance development in S. invicta in Guangdong Province. Numerous identified insecticide-related genes, GO terms, and KEGG pathways indicated the resistance of S. invicta workers to both insecticides. Importantly, this transcriptome profile variability serves as a starting point for future research on insecticide risk evaluation and the molecular mechanism of insecticide detoxification in invasive red imported fire ants.

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Section: Discussionmentioning

confidence: 99%

Transcriptome analysis reveals differential effects of beta-cypermethrin and fipronil insecticides on detoxification mechanisms in Solenopsis invicta

Siddiqui

Luo

Sheikh³

et al. 2022

Front. Physiol.

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“…Other members of IRAC group 30, such as the isoxazolines fluralaner, fluxametamide and isocycloseram, and the meta-diamide broflanilide, previously were shown to exhibit excellent acaricidal activity against T. urticae, with in some cases even higher activity compared to commercially available acaricides. 31,32,[42][43][44][45] Both fluralaner and broflanilide were found to act on insect GABACls by using either binding assays with [(3)H]ethynylbicycloorthobenzoate (EBOB) and/or TEVC assays, respectively. 23,46 The same was observed for fluxametamide, confirming that isoxazolines and meta-diamides have insect GABACls as a target.…”

Section: Discussionmentioning

confidence: 99%

“…demonstrated the lack of cross‐resistance with cyclodienes using WT and cyclodiene‐resistant strains of Drosophila . Other members of IRAC group 30, such as the isoxazolines fluralaner, fluxametamide and isocycloseram, and the meta‐diamide broflanilide, previously were shown to exhibit excellent acaricidal activity against T. urticae , with in some cases even higher activity compared to commercially available acaricides 31,32,42–45 …”

Section: Discussionmentioning

confidence: 99%

Activity, selection response and molecular mode of action of the isoxazoline afoxolaner in Tetranychus urticae

Mermans

Dermauw

Geibel

et al. 2022

Pest Management Science

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BACKGROUND Afoxolaner is a novel representative of the isoxazolines, a class of ectoparasiticides which has been commercialized for the control of tick and flea infestations in dogs. In this study, the biological efficacy of afoxolaner against the two‐spotted spider mite Tetranychus urticae was evaluated. Furthermore, as isoxazolines are known inhibitors of γ‐aminobutyric acid‐gated chloride channels (GABACls), the molecular mode of action of afoxolaner on T. urticae GABACls (TuRdls) was studied using functional expression in Xenopus oocytes followed by two‐electrode voltage‐clamp (TEVC) electrophysiology, and results were compared with inhibition by fluralaner, fipronil and endosulfan. To examine the influence of known GABACl resistance mutations, H301A, I305T and A350T substitutions in TuRdl1 and a S301A substitution in TuRdl2 were introduced. RESULTS Bioasassays revealed excellent efficacy of afoxolaner against all developmental stages and no cross‐resistance was found in a panel of strains resistant to most currently used acaricides. Laboratory selection over a period of 3 years did not result in resistance. TEVC revealed clear antagonistic activity of afoxolaner and fluralaner for all homomeric TuRdl1/2/3 channels. The introduction of single, double or triple mutations to TuRdl1 and TuRdl2 did not lower channel sensitivity. By contrast, both endosulfan and fipronil had minimal antagonistic activities against TuRdl1/2/3, and channels carrying single mutations, whereas the sensitivity of double and triple TuRdl1 mutants was significantly increased. CONCLUSIONS Our results demonstrate that afoxolaner is a potent antagonist of GABACls of T. urticae and has a powerful mode of action to control spider mites. © 2022 Society of Chemical Industry.

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“…Many studies have documented different sublethal effects of insecticides on predatory arthropods. For example, the life history traits and fecundity of Propylea japonica were unaffected after sublethal exposure to fluralaner . The longevity and fecundity of Podisus maculiventris were significantly reduced, while the developmental duration and body weight of the predator were unaffected by sublethal concentrations of imidacloprid .…”

Section: Introductionmentioning

confidence: 97%

“…For example, the life history traits and fecundity of Propylea japonica were unaffected after sublethal exposure to fluralaner. 5 The longevity and fecundity of Podisus maculiventris were significantly reduced, while the developmental duration and body weight of the predator were unaffected by sublethal concentrations of imidacloprid. 6 The fecundity and life table parameters were negatively impacted by sublethal doses of thiamethoxam in Coccinella septempunctata.…”

Section: ■ Introductionmentioning

confidence: 98%

Detrimental Impact of λ-Cyhalothrin on the Biocontrol Efficacy of Eocanthecona furcellata by Affecting Global Transcriptome and Predatory Behavior

Yao

Quan

et al. 2022

J. Agric. Food Chem.

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Whether and how insecticide exposure will affect the biological control efficacy of predatory arthropods is critical in insecticide toxicology research but largely unexplored. In the current study, reduced biocontrol efficacy was observed in a predatory stink bugEocanthecona furcellataafter insecticide application in the field. Thus, we constructed a comparative transcriptome analysis and identified a total of 4364 upregulated and 1043 down regulated differentially expressed genes following the sublethal exposure of λ-cyhalothrin. The reduced juvenile hormone (JH) titer and increased trehalose content were observed. The predation capacity and theoretical maximum predation of predators were decreased by 31.08 and 48.90% in response to λ-cyhalothrin, respectively. Furthermore, JH supplementation after λ-cyhalothrin treatment could significantly stimulate trehalase and detoxification enzyme activities, as well as restore the predatory ability of E. furcellata. Our results help to understand the toxicological mechanism of predatory stink bug species in responding to insecticides, benefit predators' ecological services, and optimize the insecticide selection.

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Toxicity of fluralaner against vegetable pests and its sublethal impact on a biocontrol predatory ladybeetle

Cited by 14 publications

References 41 publications

Transcriptome analysis reveals differential effects of beta-cypermethrin and fipronil insecticides on detoxification mechanisms in Solenopsis invicta

Transcriptome analysis reveals differential effects of beta-cypermethrin and fipronil insecticides on detoxification mechanisms in Solenopsis invicta

Activity, selection response and molecular mode of action of the isoxazoline afoxolaner in Tetranychus urticae

Detrimental Impact of λ-Cyhalothrin on the Biocontrol Efficacy of Eocanthecona furcellata by Affecting Global Transcriptome and Predatory Behavior

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