Toxicity of melanin-free ink of Sepia officinalis to transformed cell lines: identification of the active factor as tyrosinase

Russo, Gian Luigi; Nisco, Elio De; Fiore, Gabriella; Donato, Paola Di; d’Ischia, Marco; Palumbo, Anna

doi:10.1016/s0006-291x(03)01379-2

Cited by 46 publications

(35 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Despite its effectiveness against predators, both in the present study and in a related study using longfin squid (Staudinger et al, 2011), inking was used less often than deimatic displays and flight. This is possibly due to the relatively high metabolic cost of pro-ducing and expelling ink in combination with mucus and melanin (Russo et al, 2003).…”

Section: Inkingmentioning

confidence: 99%

Defensive Responses of Cuttlefish to Different Teleost Predators

Staudinger

Buresch

Mäthger

et al. 2013

The Biological Bulletin

View full text Add to dashboard Cite

Abstract. We evaluated cuttlefish (Sepia officinalis) responses to three teleost predators: bluefish (Pomatomus saltatrix), summer flounder (Paralichthys dentatus), and black seabass (Centropristis striata). We hypothesized that the distinct body shapes, swimming behaviors, and predation tactics exhibited by the three fishes would elicit markedly different antipredator responses by cuttlefish. Over the course of 25 predator-prey behavioral trials, 3 primary and 15 secondary defense behaviors of cuttlefish were shown to predators. In contrast, secondary defenses were not shown during control trials in which predators were absent. With seabass-a benthic, sit-and-pursue predator-cuttlefish used flight and spent more time swimming in the water column than with other predators. With bluefish-an active, pelagic searching predator-cuttlefish remained closely associated with the substrate and relied more on cryptic behaviors. Startle (deimatic) displays were the most frequent secondary defense shown to seabass and bluefish, particularly the Dark eye ring and Deimatic spot displays. We were unable to evaluate secondary defenses by cuttlefish to flounder-a lie-and-wait predator-because flounder did not pursue cuttlefish or make attacks. Nonetheless, cuttlefish used primary defense during flounder trials, alternating between cryptic still and moving behaviors. Overall, our results suggest that cuttlefish may vary their behavior in the presence of different teleost predators: cryptic behaviors may be more important in the presence of active searching predators (e.g., bluefish), while conspicuous movements such as swimming in the water column and startle displays may be more prevalent with relatively sedentary, bottomassociated predators (e.g., seabass).

show abstract

Section: Inkingmentioning

confidence: 99%

Defensive Responses of Cuttlefish to Different Teleost Predators

Staudinger

Buresch

Mäthger

et al. 2013

The Biological Bulletin

View full text Add to dashboard Cite

show abstract

“…Derby 2007). For example, tyrosinase, a cytotoxin present in the ink of several cephalopods, may irritate chemosensory receptors and thus deter predators (Prota et al 1981;Russo et al 2003). Paralarvae of many octopuses can expel ink upon hatching and during their tenure in the water column (reviewed by Villanueva and Norman 2008).…”

Section: Introductionmentioning

confidence: 99%

Chemical defense in pelagic octopus paralarvae: Tetrodotoxin alone does not protect individual paralarvae of the greater blue-ringed octopus (Hapalochlaena lunulata) from common reef predators

et al. 2011

View full text Add to dashboard Cite

Some pelagic marine larvae possess anti-predator chemical defenses. Occasionally, toxic adults imbue their young with their own defensive cocktails. We examined paralarvae of the greater blue-ringed octopus (Hapalochlaena lunulata) for the deadly neurotoxin tetrodotoxin (TTX), and if present, whether TTX conferred protection to individual paralarvae. Paralarvae of H. lunulata possessed 150 ± 17 ng TTX each. These paralarvae appeared distasteful to a variety of fish and stomatopod predators, yet food items spiked with 200 ng TTX were readily consumed by predators. We conclude that TTX alone does not confer individual protection to paralarvae of H. lunulata, and that they possess an alternative defense. In larger doses, tetrodotoxin is a deterrent to the predatory stomatopod Haptosquilla trispinosa (mean dose = 3.97 lg/g). This corresponds to 12-13 paralarvae per predator based on the TTX levels of the clutch we examined. Thus, the basic assumption that individual paralarvae of H. lunulata are defended by TTX alone was disproved. Instead, functionality of TTX levels in paralarvae may arise through alternative selective pathways, such as deterrence to parasites, through kin selection, or against predator species not tested here.

show abstract

“…Several active components, including a tyrosinase and an angiotensin-converting-enzyme inhibitor have been identified [11][12][13][14]. Squid belongs to the invertebrate Mollusca and, like other cephalopods, relies on ejection of dark ink for defense.…”

Section: Introductionmentioning

confidence: 99%

Sequence determination of a non-sulfated glycosaminoglycan-like polysaccharide from melanin-free ink of the squid Ommastrephes bartrami by negative-ion electrospray tandem mass spectrometry and NMR spectroscopy

Chen

Xue

et al. 2008

Glycoconj J

View full text Add to dashboard Cite

A non-sulfated polysaccharide was isolated from the ink sac of squid Ommastrephes bartrami after removal of the melanin granules. The carbohydrate sequence of this polysaccharide was assigned by negative-ion electrospray tandem mass spectrometry with collision-induced dissociation of the oligosaccharide fractions produced by partial acid hydrolysis of the polysaccharide. The structural determination was completed by NMR for assignment of anomeric configuration and confirmation of linkage information and it was unambiguously identified as a glycosaminoglycan-like polysaccharide containing a glucuronic acid-fucose (GlcA-Fuc) disaccharide repeat in the main chain and a N-acetylgalactosamine (GalNAc) branch at Fuc position 3: -[3GlcAbeta1-4(GalNAcalpha1-3)Fucalpha1](n)-. Partial hydrolysis of the polysaccharide to obtain several oligosaccharide fractions with different numbers of the repeating unit assisted the assignment. In the negative-ion tandem mass spectrometric analysis, the unique (0,2)A type fragmentation was important to establish the presence of a 4-linked fucose in the main polysaccharide chain and a GalNAc branch at the Fuc position-3 of the disaccharide repeat.

show abstract

Toxicity of melanin-free ink of Sepia officinalis to transformed cell lines: identification of the active factor as tyrosinase

Cited by 46 publications

References 31 publications

Defensive Responses of Cuttlefish to Different Teleost Predators

Defensive Responses of Cuttlefish to Different Teleost Predators

Chemical defense in pelagic octopus paralarvae: Tetrodotoxin alone does not protect individual paralarvae of the greater blue-ringed octopus (Hapalochlaena lunulata) from common reef predators

Sequence determination of a non-sulfated glycosaminoglycan-like polysaccharide from melanin-free ink of the squid Ommastrephes bartrami by negative-ion electrospray tandem mass spectrometry and NMR spectroscopy

Contact Info

Product

Resources

About