Toxicity of phenol solutions treated with rapeseed and tomato hairy roots

Paisio, Cintia Elizabeth; González, Pablo J.; Gerbaudo, Alejandra; Bertuzzi, Mabel; Agostini, Elizabeth

doi:10.1016/j.desal.2010.06.029

Cited by 17 publications

(16 citation statements)

References 27 publications

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“…These results are consistent with those described by Paisio et al (2010) which also showed that the toxicity of 100 and 250 mg/L phenol solutions treated with B. napus HR, decreased significantly compared to phenol solutions without treatment, using the AMPHITOX bioassay.…”

Section: Discussionsupporting

confidence: 95%

Evaluation of phenol detoxification by Brassica napus hairy roots, using Allium cepa test

González

Maglione

Giordana

et al. 2011

Environ Sci Pollut Res

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In the present work, Brassica napus hairy roots (HR) were used for phenol removal assays. The toxicity of post-removal solutions (PRS) and phenol solutions was analyzed. These HR removed the contaminant with high efficiency (100-80% for phenol solutions containing 10-250 mg/L, respectively). Phenol solutions treated with B. napus HR showed a significant reduction of general toxicity compared to untreated phenol solutions, since the IC50 values were 318.39 and 229.02 mg/L, respectively. Moreover, PRS presented lower cytotoxicity and genotoxicity than that found in phenol solutions untreated. The mitotic index (MI) observed in meristematic cells treated with PRS (100 and 250 mg/L of phenol) showed an increase of 35% and 42%, whereas the chromosome aberrations showed a significant decrease. According to these results, B. napus HR cultures could be used for the treatment of solutions contaminated with phenol, since we observed not only high removal efficiency, but also an important reduction of the general toxicity, cytotoxicity, and genotoxicity.

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Section: Discussionsupporting

confidence: 95%

Evaluation of phenol detoxification by Brassica napus hairy roots, using Allium cepa test

González

Maglione

Giordana

et al. 2011

Environ Sci Pollut Res

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“…Similarly, phenol removal by tomato hairy roots displayed comparable efficiency in PEG-supplemented and PEG-unsupplemented reaction mixtures. By contrast, adding PEG to the assay medium significantly increased phenol removal by rapeseed hairy roots (Paisio et al 2010 ). Similarly, González et al ( 2008 ) reported that PEG supplementation improves the efficiency of the reaction with tomato hairy roots.…”

Section: Resultsmentioning

confidence: 99%

“…Besides the preparations of purified enzyme (immobilized or free), several peroxidase-producing systems have been considered for use in phenol remediation. Hairy root cultures have been tested for their ability to remove phenol or dichlorophenol (González et al 2006 , 2008 , 2012 ; Singh et al 2006 ; Paisio et al 2010 ; Sosa Alderete et al 2012 ; Jha et al 2013 ). Hairy roots offer an attractive system for this purpose due to their ability to produce large quantities of exudates that are rich in peroxidase and chelating agents.…”

Section: Introductionmentioning

confidence: 99%

Removal of Phenol from Synthetic and Industrial Wastewater by Potato Pulp Peroxidases

Kurnik

Treder

Skorupa-Kłaput

et al. 2015

Water Air Soil Pollut

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Plant peroxidases have strong potential utility for decontamination of phenol-polluted wastewater. However, large-scale use of these enzymes for phenol depollution requires a source of cheap, abundant, and easily accessible peroxidase-containing material. In this study, we show that potato pulp, a waste product of the starch industry, contains large amounts of active peroxidases. We demonstrate that potato pulp may serve as a tool for peroxidase-based remediation of phenol pollution. The phenol removal efficiency of potato pulp was over 95 % for optimized phenol concentrations. The potato pulp enzymes maintained their activity at pH 4 to 8 and were stable over a wide temperature range. Phenol solutions treated with potato pulp showed a significant reduction in toxicity compared with untreated phenol solutions. Finally we determined that this method may be employed to remove phenol from industrial effluent with over 90 % removal efficiency under optimal conditions.

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“…In nature, quinones can appear as a result of oxidative transformation of phenols, a numerous group of hydroxylated aromatic compounds, that are known to be the third in the top list of widespread pollutants (after metal salts and oil products). Phenols are frequent components in wastewaters of chemical recovery, organic synthesis, as well as hydrolytic, cellulose, and flax industries (Park et al 2012;Paisio et al 2010;Shourian et al 2009;Ren 2003). A range of phenolic substances are also synthesized and extracellularly excreted by many soil bacteria; the substances are used as molecular signals in microbial communication and as adaptogens (Stasiuk & Kozubek, 2010;El'-Registan et al 2006) and are Fig.…”

Section: Detoxification Of Oxidizer Solutionsmentioning

confidence: 99%

Pollutant toxicity and detoxification by humic substances: mechanisms and quantitative assessment via luminescent biomonitoring

Kudryasheva

Tarasova

2014

Environ Sci Pollut Res

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The paper considers mechanisms of detoxification of pollutant solutions by water-soluble humic substances (HSs), natural detoxifying agents. The problems and perspectives of bioassay application for toxicity monitoring of complex solutions are discussed from ecological point of view. Bioluminescence assays based on marine bacteria and their enzymes are of special attention here; they were shown to be convenient tools to study the detoxifying effects on cellular and biochemical levels. The advantages of bioluminescent enzymatic assay for monitoring both integral and oxidative toxicities in complex solutions of model pollutants and HS were demonstrated. The efficiencies of detoxification of the solutions of organic oxidizers and salts of metals (including radioactive ones) by HS were analyzed. The dependencies of detoxification efficiency on time of exposure to HS and HS concentrations were demonstrated. Antioxidant properties of HS were considered in detail. The detoxifying effects of HS were shown to be complex and regarded as 'external' (binding and redox processes in solutions outside the organisms) and/or 'internal' organismal processes. The paper demonstrates that the HS can stimulate a protective response of bacterial cells as a result of (1) changes of rates of biochemical reactions and (2) stabilization of mucous layers outside the cell walls. Acceleration of auto-oxidation of NADH, endogenous reducer, by HS was suggested as a reason for toxicity increase in the presence of HS due to abatement of reduction ability of intracellular media.

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Toxicity of phenol solutions treated with rapeseed and tomato hairy roots

Cited by 17 publications

References 27 publications

Evaluation of phenol detoxification by Brassica napus hairy roots, using Allium cepa test

Evaluation of phenol detoxification by Brassica napus hairy roots, using Allium cepa test

Removal of Phenol from Synthetic and Industrial Wastewater by Potato Pulp Peroxidases

Pollutant toxicity and detoxification by humic substances: mechanisms and quantitative assessment via luminescent biomonitoring

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