Toxicity of silver nanoparticle in rat ear and BALB/c 3T3 cell line

Zou, Jing; Feng, Hao; Mannerström, Marika; Heinonen, Tuula; Pyykkö, Ilmari

doi:10.1186/s12951-014-0052-6

Cited by 42 publications

(37 citation statements)

References 37 publications

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“…Regardless of the outcome of the in vitro study, we should also remember that the mouse 3T3 cell culture line may be many times more sensitive than the cells examined under in vivo conditions . Another important recommendation when working with the 3T3 cells is that the response of 3T3 fibroblasts to oxidative stress, which is one of the mechanisms of cell death, may undergo alterations after long‐term cell exposure to endocrine disruptors …”

Section: Discussionmentioning

confidence: 99%

“…The utility of the 3T3 cell line has been shown in many other biological and toxicological studies, using various experimental approaches. For example, 3T3 fibroblasts have been selected for the following purposes: 1/investigating the activity of bacterial toxins, antioxidants and cytokines, 2/evaluating the toxicity of drugs, e‐waste leachate, nanomaterials and nanoparticles or 3/studying the beneficial effects of electromagnetic radiation in living cells . These cells can also serve as a tool for the verification and validation of techniques assessing mitochondrial toxicity .…”

Section: Introductionmentioning

confidence: 99%

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Differentiated mitochondrial function in mouse 3T3 fibroblasts and human epithelial or endothelial cells in response to chemical exposure

Boncler

Łukasiak²,

Dastych

et al. 2018

Basic Clin Pharma Tox

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Mouse 3T3 fibroblasts are commonly used for in vitro toxicity testing; however, their sensitivity to stimuli is not well defined. To assess the sensitivity of the 3T3 cell line, the study compared the changes in mitochondrial membrane potential (MMP) occurring after exposure to eight chemicals known to demonstrate pro-apoptotic activity (glycerol, isopropanol, ethanol, paracetamol, propranolol, cobalt chloride, formaldehyde and atropine). Five cell lines were used as follows: mouse 3T3 fibroblasts, human epithelial cells (A549, Caco-2 and HepG2) and human endothelial cells (HMEC-1). Cell sensitivity was assessed based on the total area under and over the dose-response curves (AUOC) in relation to baselines. The 3T3 fibroblasts had the highest AUOC values and were the most sensitive to the action of all the examined chemicals, with the exception of formaldehyde. Significant changes in MMP between the 3T3 cell line and other cells were observed after cell treatment with atropine (A549, Caco-2 or HMEC-1 cells vs 3T3 cells, P < 0.05), propranolol (A549 vs 3T3 cells, P < 0.01; HepG2 vs 3T3 cells, P < 0.05), cobalt chloride (A549 cells vs 3T3 cells, P < 0.01) or ethanol (HMEC-1 vs 3T3, P < 0.05). Formaldehyde appeared the most toxic compound for Caco-2 cells (Caco-2 vs 3T3 cells, P < 0.05). The surface areas (AUOC) calculated for each other chemical and obtained for HepG2, Caco-2, A549 and HMEC-1 did not differ significantly between cell lines. We postulate that mouse 3T3 fibroblasts demonstrate significantly higher relative sensitivity to many agents with toxic potential.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Differentiated mitochondrial function in mouse 3T3 fibroblasts and human epithelial or endothelial cells in response to chemical exposure

Boncler

Łukasiak²,

Dastych

et al. 2018

Basic Clin Pharma Tox

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“…Neutral red uptake assay, originally developed by Borenfreund and Puerner , was performed using the standard protocol of OECD Guidance document 129 and as described in Zou et al . . Briefly, 25 μg/ml NR solution was added to the cells and blanks and the plates were incubated at 37°C, 5% CO 2 for 3.0 ± 0.1 hr.…”

Section: Methodsmentioning

confidence: 99%

Human BJ Fibroblasts is an Alternative to Mouse BALB/c 3T3 Cells in In Vitro Neutral Red Uptake Assay

Mannerström

Toimela

Sarkanen

et al. 2017

Basic Clin Pharma Tox

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The OECD GD 129 BALB/c 3T3 neutral red uptake (NRU) assay is a standardized test method for estimating starting dose for an acute oral systemic toxicity test in rodents. Mouse BALB/c 3T3 fibroblasts are the most commonly used cells in the NRU assay. We have previously transferred and validated BALB/c 3T3 NRU assay in our GLP laboratory. Subsequently, in order to obtain more human-relevant cytotoxicity data, we performed an intralaboratory validation using human BJ fibroblasts in the NRU assay instead of mouse BALB/c 3T3 fibroblasts. Here, we present comparative cytotoxicity data of 26 different test chemicals (pharmaceuticals, industrial chemicals, pesticides and food additives) produced with both BALB/c 3T3 NRU and BJ NRU assays.

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“…In in vitro studies, Ag NPs were used for MRI imaging. Their toxic effect on the structure of the ear was investigated in rats; they reduced hearing frequency in rats, were effective on the mitochondrial membrane and increased ROS level [175].…”

Section: Metal Npsmentioning

confidence: 99%

“…After 5 h, NP penetration into cells was observed which caused mitochondrial dysfunction; the toxicity was dose-and time-dependent [175].…”

Section: Metal Npsmentioning

confidence: 99%

Health concerns of various nanoparticles: A review of their in vitro and in vivo toxicity

Ajdari¹,

Moosavi²,

Rahmati³

et al. 2018

Preprint

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Nanoparticles (NPs) are widely used in diverse disciplines, including biology, medicine science. The central question that need to be answered is whether NPs have toxic effects on biological cells and molecules or are they safe. The safety of NPs including targeted drug delivery is critical and so is their toxicity in the environment. In recent years, in vitro and in vivo research on animals has generated abundant information about the toxicity of NPs.However, due to varying laboratory conditions, the comparison of the results from ensuing studies is somewhat unreliable. It should be noted that, depending on the type of production, NPs can enter the body through inhalation, skin and via digestive routes. Due to the diversity of NPs and their properties, there is paucity of accurate information on their toxicological effects; particle size, shape, surface area and the chemical levels are considered as key factors in creating health and toxicological effects. Consequently, there is a need for reliable information about their effects on various organs so as to deal with NPs effectively and their impact on health and the environment. This review covers the existing knowledge base on the subject that hopefully prepares us better to address these challenges.

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Toxicity of silver nanoparticle in rat ear and BALB/c 3T3 cell line

Abstract: Background: Silver nanoparticles (AgNPs) displayed strong activities in anti-bacterial, anti-viral, and anti-fungal studies and was reportedly efficient in treating otitis media .The potential impact of AgNPs on the inner ear was missing.

Cited by 42 publications

References 37 publications

Differentiated mitochondrial function in mouse 3T3 fibroblasts and human epithelial or endothelial cells in response to chemical exposure

Differentiated mitochondrial function in mouse 3T3 fibroblasts and human epithelial or endothelial cells in response to chemical exposure

Human BJ Fibroblasts is an Alternative to Mouse BALB/c 3T3 Cells in In Vitro Neutral Red Uptake Assay

Health concerns of various nanoparticles: A review of their in vitro and in vivo toxicity

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