Toxicity of the mycotoxin fumonisin B 1 on the insect Sf9 cell line

Zhang, He; Zhang, Jintao; Diao, Xue; Li, Na; Liu, Chenglan

doi:10.1016/j.toxicon.2017.01.018

Cited by 11 publications

(8 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The application of fumonisin B2 to the Sf-9 cell cultures in the present study inhibited cell proliferation and resulted in the appearance of spindle cells ( Table 1, Fig 2). Similar effects were reported by Zhang and co-workers, who report Sf-9 cell growth to be arrested at the G 2 /M phase, loss of adhesion, swelling and vacuole formation, depolarization of the cell membrane potential and hyperpolarization of the mitochondrial membrane potential following fumonisin B1 treatment [33]. The difference in susceptibility of the insect Sf-9 cells against the tested mycotoxins compared with that of the mammalian Caco-2 and THP-1 cells can be attributed to differences in cell lipid composition.…”

Section: Plos Onesupporting

confidence: 88%

“…Alternariol, ochratoxin A, patulin, and sterigmatocystin cannot be considered as future with minor impact on Caco-2 and THP-1 cells, they are nevertheless known to display high toxicity and carcinogenicity in animals and humans [54][55][56][57]; the same applies to fumonisin, proposed by Zand and co-workers as bio-insecticide [33]. In addition, brefeldin and T2 tetraol show little potential for the control of insect pests as they have been found to stimulate the proliferation of human monocytic leukemia THP-1 cells ( Table 1).…”

Section: Plos Onementioning

confidence: 99%

See 1 more Smart Citation

In vitro screening of 65 mycotoxins for insecticidal potential

et al. 2021

View full text Add to dashboard Cite

The economic losses and threats to human and animal health caused by insects and the pathogens transmitted by them require effective and environmentally-friendly methods of controlling them. One such group of natural biocontrol agents which may be used as biopesticides is that of the entomopathogenic fungi and their toxic secondary metabolites (mycotoxins). The present in vitro work examined the insecticidal potential of 65 commercially-available mycotoxins against the insect Sf-9 cell line. Mammalian Caco-2 and THP-1 cell lines served as reference controls to select insecticidal mycotoxins harmless to mammalian cells. All tested mycotoxins significantly reduced the in vitro proliferation of the Sf-9 cells and evoked morphological changes. Ten of the mycotoxins found to strongly inhibit Sf-9 proliferation also had moderate or no effect on Caco-2 cells. The THP-1 cells were highly resistant to the tested mycotoxins: doses 103 times higher were needed to affect viability and morphology (1 μg/ml for THP-1 versus 1 ng/ml for Sf-9 and Caco-2). Nine mycotoxins significantly decreased Sf-9 cell proliferation with minor effects on mammalian cells: cyclosporins B and D, cytochalasin E, gliotoxin, HC toxin, paxilline, penitrem A, stachybotrylactam and verruculogen. These may be good candidates for future biopesticide formulations.

show abstract

Section: Plos Onesupporting

confidence: 88%

Section: Plos Onementioning

confidence: 99%

In vitro screening of 65 mycotoxins for insecticidal potential

et al. 2021

View full text Add to dashboard Cite

show abstract

“…According to the phylogenetic analysis of P450s, 29, 39 and 8 CYPs were clustered into CYP4, CYP6 and CYP9 subfamily with CYP4s CYP6s and CYP9s from other insect species. CYP6AE19 transcripts were strongly induced when H. armigera was exposed to methyl jasmonate, and fumonisin B1 exposure in S. frugiperda makes upregulation of 17 P450 genes, including CYP6AE44 (Berenbaum et al 2021;Elzaki et al 2019;Zhang et al 2017). In B. mori, CYP4G22 was identified to have a positive upregulation after induction of imidacloprid (Yamamoto et al 2010).…”

Section: Discussionmentioning

confidence: 98%

Transcriptome characterization and profiling related to detoxification enzyme genes in Chilo sacchariphagus (Lepidoptera Crambidae)

Liu

et al. 2022

Entomological Research

View full text Add to dashboard Cite

Chilo sacchariphagus Bojeris is one of the most dangerous pests of sugarcane. The larvae damage the seedlings and stems of sugarcane and also harm sorghum, corn and other crops, which causes great economic losses to the sugar industry every year. Transcriptome sequencing and expression profile analysis of cytochrome P450 monooxygenase (CYP), carboxylesterase and glutathione S-transferase (GST) were carried out, which could provide a basis for drug resistance monitoring and drug resistance management of the pest. Unigenes of C. sacchariphagus were obtained by using the Illumina HiSeqTM 4,000 platform as 150 bp paired-end reads. A total of 173,013 unigenes were obtained after data assembly and redundancy removal. 28,330 unigenes were annotated based on multiple public databases, and the number of unigenes annotated by NR database was the largest. According to the transcriptome analysis, 214 candidate detoxification enzyme genes were identified, including 44 GSTs, 138 CYPs, and 32 CarEs. Phylogenetic analysis showed that the CYPs were mainly clustered in CYP4, CYP6, CYP9 and CYP12 subfamilies; CarEs mainly include antennal CarEs, venom CarEs and CarEs NOTUM; while the GSTs cluster mainly contains subfamilies such as delta, omega, epsilon, theta, zeta and sigma. In this study, transcriptome information of C. sacchariphagus was obtained, and genes related to detoxification were identified, which could provide data and a basis for the further study of detoxification and host plant adaptation mechanism of C. sacchariphagus.

show abstract

“…Морфология клеток менялась под его воздействием: набухание, образование вакуолей и потери способности к адгезии. Флоуцитометрический анализ показал, что токсин приводил к деполяризации и гиперполяризацию мембранного потенциала оболочки митохондрий (Zhang et al, 2017).…”

Section: почвенные грибыunclassified

Promising approaches to the search for fungal metabolites for management of arthropod pests

Берестецкий¹,

Lednev²,

Ху

2021

1727-1320

View full text Add to dashboard Cite

Biorational insecticides of natural origin, such as avermectins, spinosins, azadirachtin and afidopyropen, are increasingly used in agriculture. The present paper reviews modern ecological, genomic, and biotechnological approaches to the search for new compounds with insecticidal properties (entomotoxic, antifeedant, and hormonal) produced by fungi of various ecological groups (entomopathogens, soil saprotrophs, endophytes, phytopathogens, and mushrooms). The literature survey showed that insecticidal metabolites of entomopathogenic fungi had not been sufficiently studied, and the majority of well-characterized compounds had showed moderate insecticidal activity. The greatest number of substances with insecticidal properties was found to be produced by soil fungi, mainly from the genera Aspergillus and Penicillium. Metabolites with insecticidal and antifeedant properties were also found in endophytic and phytopathogenic fungi. Low sensitivity of insect pests of stored products (in particular, of grain) to mycotoxins was recorded. Mushrooms were found to be promising producers of antifeedant compounds as well as insecticidal proteins. It is possible to increase the number of substances with insecticidal properties detected in fungi not only by extension of the screening range but also by exploitation of diverse bioassay sytems and model insect species. Mining genomes for secondary metabolite gene clusters and secreted proteins with their subsequent activation by various methods allows for better understanding of the biosynthetic potential of the prospective strains. Efficacy of these studies can be increased with high-throughput techniques of fungal metabolites extraction and further analysis using chromatography and mass spectrometry. Insecticidal proteins detected in fungi can be used in the technologies for development of transgenic plant varieties resistant to pests, or hypervirulent bioinsecticides.

show abstract

Toxicity of the mycotoxin fumonisin B 1 on the insect Sf9 cell line

Cited by 11 publications

References 27 publications

In vitro screening of 65 mycotoxins for insecticidal potential

In vitro screening of 65 mycotoxins for insecticidal potential

Transcriptome characterization and profiling related to detoxification enzyme genes in Chilo sacchariphagus (Lepidoptera Crambidae)

Promising approaches to the search for fungal metabolites for management of arthropod pests

Contact Info

Product

Resources

About